Altered Patterns of DNA Methylation on Chromosomes from Leukemia Cell Lines: Identification of 5-Methylcytosines by Indirect Immunodetection

Bensaada, Mustapha; Kiefer, Hélène; Tachdjian, Gérard; Lapierre, Jean-Michel; Cacheux, Valère; Niveleau, Alain; Metezeau, Philippe

doi:10.1016/s0165-4608(97)00409-3

Cited by 21 publications

(8 citation statements)

References 42 publications

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“…Many DNA methylation quantification methods provide information about site-specific or in situ methylation, but the quantification of total DNA methylation has received less attention [6,7]. The relative percentage of 5-methylcytosine (mC) in genomic DNA allows information to be obtained about changes in chromatin structure.…”

Section: Introductionmentioning

confidence: 99%

High-performance capillary electrophoretic method for the quantification of 5-methyl 2'-deoxycytidine in genomic DNA: Application to plant, animal and human cancer tissues

et al. 2002

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A new approach to the evaluation of the relative degree of genomic DNA methylation through the quantification of 2'-deoxynucleosides is proposed. Detection and quantification of 5-methyl 2'-deoxycytidine in genomic DNA has been performed using micellar high-performance capillary electrophoresis (HPCE) with UV-Vis detection. This approach has been demonstrated to be more sensitive and specific than other HPCE methods for the quantification of DNA methylation degree and also to be faster than other HPLC-based methods. The detection and quantification of nucleosides through enzymatic hydrolyses notably increases the specificity of the technique and allows its exploitation in the analysis of poorly purified and/or concentrated DNA samples such as those obtained from meristematic plant regions and paraffin-embedded tissues.

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Section: Introductionmentioning

confidence: 99%

High-performance capillary electrophoretic method for the quantification of 5-methyl 2'-deoxycytidine in genomic DNA: Application to plant, animal and human cancer tissues

et al. 2002

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“…In these species, the methylated regions corresponded to the locations of repetitive DNA, i.e., to the heterochromatic regions of all or a subset of the chromosomes in the karyotypes. Subsequently, this technique was applied to chromosomes of further mammalian species [VasilikakiBaker and Nishioka, 1983;Bernardino et al, 2000] and to human chromosomes [Barbin et al, 1994;Bernardino et al, 1996;Kokalj-Vokac et al, 1998], even including cases of inherited chromosome aberrations [Breg et al, 1974] and leukemia cell lines [Bensaada et al, 1998]. …”

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confidence: 99%

The Hypermethylated Regions in Avian Chromosomes

Schmid

Steinlein²

2017

Cytogenet Genome Res

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Chromosomal locations and amounts of 5-methylcytosine-rich chromosome regions were detected in the karyotypes of 13 bird species by indirect immunofluorescence using a monoclonal anti-5-methylcytosine antibody. These species belong to 7 orders and 10 families of modern (Neognathae) and primitive (Palaeognathae) birds and are characterized by macro- and microchromosomes as well as ZW sex chromosomes. In all 13 species, the hypermethylated chromosome segments are confined to constitutive heterochromatin. The chromosomal locations of hypermethylated DNA regions in the karyotypes are constant and species-specific. There is no general rule with regard to the distribution of these hypermethylated chromosome regions in the genomes of birds. In most instances, hypermethylated segments are located in the centromeric regions of chromosomes, but in the sex chromosomes, these can also be found in telomeric and interstitial postitions. In most of the species studied, the centromeric heterochromatin in many, if not all, of the microchromosomes is hypermethylated. However, in one species, the only detectable hypermethylated heterochromatic regions are located in one pair of macroautosomes and in the Z sex chromosome, but none of the microchromosomes contains visible quantities of 5-methylcytosine. The analysis of 5-methylcytosine-rich chromosome regions can be very helpful for the comparative cytogenetics of closely related species or subspecies. It also reflects the dynamic evolutionary process operating in the highly repetitive DNA of eukaryotic chromosomes.

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“…to the heterochromatic regions of all or a subset of the chromosomes in the karyotypes. Subsequently, this technique was applied to chromosomes of further mammalian species [Vasilikaki-Baker and Nishioka, 1983;Bernardino et al, 2000] and to human chromosomes [Barbin et al, 1994;Bernardino et al, 1996;Kokalj-Vokac et al, 1998], even including cases of inherited chromosome aberrations [Breg et al, 1974] and leukemia cell lines [Bensaada et al, 1998]. In birds, studies using anti-5-MeC antibodies were performed on the chromosomes of the chicken [Grützner et al, 2001;Schmid et al, 2015] and 12 further avian species belonging to 7 different orders and 10 families [Schmid et al, 2015].…”

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confidence: 99%

Hypermethylated Chromosome Regions in Nine Fish Species with Heteromorphic Sex Chromosomes

Schmid

Steinlein

Yano

et al. 2015

Cytogenet Genome Res

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Sites and amounts of 5-methylcytosine (5-MeC)-rich chromosome regions were detected in the karyotypes of 9 Brazilian species of Characiformes fishes by indirect immunofluorescence using a monoclonal anti-5-MeC antibody. These species, belonging to the genera Leporinus, Triportheus and Hoplias, are characterized by highly differentiated and heteromorphic ZW and XY sex chromosomes. In all species, the hypermethylated regions are confined to constitutive heterochromatin. The number and chromosome locations of hypermethylated heterochromatic regions in the karyotypes are constant and species-specific. Generally, heterochromatic regions that are darkly stained by the C-banding technique are distinctly hypermethylated, but several of the brightly fluorescing hypermethylated regions merely exhibit moderate or faint C-banding. The ZW and XY sex chromosomes of all 9 analyzed species also show species-specific heterochromatin hypermethylation patterns. The analysis of 5-MeC-rich chromosome regions contributes valuable data for comparative cytogenetics of closely related species and highlights the dynamic process of differentiation operating in the repetitive DNA fraction of sex chromosomes.

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Altered Patterns of DNA Methylation on Chromosomes from Leukemia Cell Lines: Identification of 5-Methylcytosines by Indirect Immunodetection

Cited by 21 publications

References 42 publications

High-performance capillary electrophoretic method for the quantification of 5-methyl 2'-deoxycytidine in genomic DNA: Application to plant, animal and human cancer tissues

High-performance capillary electrophoretic method for the quantification of 5-methyl 2'-deoxycytidine in genomic DNA: Application to plant, animal and human cancer tissues

The Hypermethylated Regions in Avian Chromosomes

Hypermethylated Chromosome Regions in Nine Fish Species with Heteromorphic Sex Chromosomes

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