Alternating Current Electric Fields of Varying Frequencies: Effects on Proliferation and Differentiation of Porcine Neural Progenitor Cells

Mazurek

et al. 2017

Ann Agric Environ Med.

Electromagnetic radiation (EMR) emitting from the natural environment, as well as from the use of industrial and everyday appliances, constantly influence the human body. The effect of this type of energy on living tissues may exert various effects on their functioning, although the mechanisms conditioning this phenomenon have not been fully explained. It may be expected that the interactions between electromagnetic radiation and the living organism would depend on the amount and parameters of the transmitted energy and type of tissue exposed. Electromagnetic waves exert an influence on human reproduction by affecting the male and female reproductive systems, the developing embryo, and subsequently, the foetus. Knowledge concerning this problem is still being expanded; however, all the conditionings of human reproduction still remain unknown. The study presents the current state of knowledge concerning the problem, based on the latest scientific reports.

Section: Mechanisms Of the Effect Of Emrsupporting

confidence: 61%

Effect of electromagnetic waves on human reproduction

Mazurek

et al. 2017

Ann Agric Environ Med.

“…For example, Ariza et al found out that AC did not have a negative impact on viability versus DC which had a destructive effect on the viability of hippocampal neural progenitor cells (NPCs) . In another study, researchers used a series of AC currents (1‐10‐50 Hz) to investigate the viability and differentiation of stem cells and finally discovered that none of these AC currents affect the cell viability in 7 days . In our study, we stimulated CJMSCs using AC current with frequencies 1 and 100 Hz.…”

Section: Discussionmentioning

confidence: 86%

“…74 In another study, researchers used a series of AC currents (1-10-50 Hz) to investigate the viability and differentiation of stem cells and finally discovered that none of these AC currents affect the cell viability in 7 days. 18 In our study, we stimulated CJMSCs using AC current with frequencies 1 and 100 Hz. However, the result shows that 1-Hz frequency has more cytotoxicity than 100 Hz.…”

Section: Discussionmentioning

confidence: 99%

“…16 There are not enough studies to evaluate the effect of alternating current on neural differentiation of stem cells. 17,18 The first aim of this study was to fabricate a proportional biocompatible conductive nanofibrous scaffold from SF-rGo to evaluate its coefficient properties together with electrical stimulation on neural differentiation of conjunctiva mesenchymal stem cells (CJMSCs). The next point was to compare the neural differentiation effect of electrical stimulation with different frequencies on CJMSCs.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Conductive electrospun scaffolds with electrical stimulation for neural differentiation of conjunctiva mesenchymal stem cells

et al. 2019

An electrical stimulus is a new approach to neural differentiation of stem cells. In this work, the neural differentiation of conjunctiva mesenchymal stem cells (CJMSCs) on a new 3D conductive fibrous scaffold of silk fibroin (SF) and reduced graphene oxide (rGo) were examined. rGo (3.5% w/w) was dispersed in SF‐acid formic solution (10% w/v) and conductive nanofibrous scaffold was fabricated using the electrospinning method. SEM and TEM microscopies were used for fibrous scaffold characterization. CJMSCs were cultured on the scaffold and 2 electrical impulse models (Current 1:115 V/m, 100‐Hz frequency and current 2:115 v/m voltages, 0.1‐Hz frequency) were applied for 7 days. Also, the effect of the fibrous scaffold and electrical impulses on cell viability and neural gene expression were examined using MTT assay and qPCR analysis. Fibrous scaffold with the 220 ± 20 nm diameter and good dispersion of graphene nanosheets at the surface of nanofibers were fabricated. The MTT result showed the viability of cells on the scaffold, with current 2 lower than current 1. qPCR analysis confirmed that the expression of β‐tubulin (2.4‐fold P ≤ 0.026), MAP‐2 (1.48‐fold; P ≤ 0.03), and nestin (1.5‐fold; P ≤ 0.03) genes were higher in CJMSCs on conductive scaffold with 100‐Hz frequency compared to 0.1‐Hz frequency. Collectively, we proposed that SF‐rGo fibrous scaffolds, as a new conductive fibrous scaffold with electrical stimulation are good strategies for neural differentiation of stem cells and the type of electrical pulses has an influence on neural differentiation and proliferation of CJMSCs.

“…IDEs were connected to a signal generator (33220A; Agilent Technologies, Santa Clara, CA, USA). Stimulus intensity of 10 mV (peak‐to‐peak) was used to generate the calculated electrical fields of 1 V/cm on cells (12, 38). Waveform and intensity were confirmed with an oscilloscope.…”

Section: Methodsmentioning

confidence: 99%

Primary cilia are sensors of electrical field stimulation to induce osteogenesis of human adipose‐derived stem cells

et al. 2016

Self Cite

In this study, we report for the first time that the primary cilium acts as a crucial sensor for electrical field stimulation (EFS)-enhanced osteogenic response in osteoprogenitor cells. In addition, primary cilia seem to functionally modulate effects of EFS-induced cellular calcium oscillations. Primary cilia are organelles that have recently been implicated to play a crucial sensor role for many mechanical and chemical stimuli on stem cells. Here, we investigate the role of primary cilia in EFS-enhanced osteogenic response of human adipose-derived stem cells (hASCs) by knocking down 2 primary cilia structural proteins, polycystin-1 and intraflagellar protein-88. Our results indicate that structurally integrated primary cilia are required for detection of electrical field signals in hASCs. Furthermore, by measuring changes of cytoplasmic calcium concentration in hASCs during EFS, our findings also suggest that primary cilia may potentially function as a crucial calcium-signaling nexus in hASCs during EFS.-Cai, S., Bodle, J. C., Mathieu, P. S., Amos, A., Hamouda, M., Bernacki, S., McCarty, G., Loboa, E. G. Primary cilia are sensors of electrical field stimulation to induce osteogenesis of human adipose-derived stem cells.