Alternating electric fields induce a period-dependent motion of<i>Escherichia coli</i>in three-dimension near a conductive surface

Zhou, Xin; Huang, Gui; Ma, Chunfeng; Bao, Lian-Jun; Gong, Xiangjun; Zhang, Guangzhao; Zeng, Eddy Y.

doi:10.1116/1.5078543

Cited by 4 publications

(8 citation statements)

References 36 publications

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“…The holograms of 1024 × 1024 pixels were recorded by an sCMOS camera (Zyla-5.5-CL3, Andor Technology, Northern Ireland, the UK) at 50 frames/s for 2 min, and then, these holograms were subtracted by a background image for further data processing to obtain the 3D bacterial locations. The following motion parameters have been described in detail by Gong et al , A bacterial motion was classified into active and subdiffusive modes in terms of the mean square displacement (MSD). N sub and N act referred to the number of subdiffusive and active bacteria, respectively.…”

Section: Methodsmentioning

confidence: 99%

Comparison of the Antiadhesion Activity and Mechanism between Different Chiral Molecules L-Borneol and D-Borneol against Escherichia coli

Wan,

Cai,

Gong

et al. 2024

ACS Food Sci. Technol.

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The biofilm of Escherichia coli begins with a reversible adhesion phase, in which the movement of the planktonic bacteria could be regulated by a chemotaxis system. It has been reported that bacteria have adhesion differences with respect to different chiral configurations of borneols, but the underlying mechanism is unclear. Here, we explored the mechanisms by which Lborneol and D-borneol influenced the adhesion of E. coli mediated by chemoreceptors using real-time tracking of three-dimensional (3D) trajectories of bacterial motility and proteomics technology. We found that both borneols could restrain the adhesion and motility activity of E. coli. However, E. coli generated only escape movement toward the L-borneol. Both L-borneol and D-borneol had the foundation for binding to E. coli chemoreceptors Tar and Tsr, according to molecular docking analysis. E. coli lost this escape maneuver when Tar or Tsr was knocked out. Proteomics revealed that E. coli under D-borneol treatment exhibited a chemotaxis trend due to the upregulated proteins of chemotaxis and the flagellar assembly. However, insufficient ATP and proton motive force (PMF) in the D-borneol-treated group prevented E. coli from accomplishing the chemotactic behavior, resulting in reduced bacterial adhesion. In contrast, E. coli showed rapid repellent migration toward L-borneol in a short time by the 3D trajectory diagram. Lborneol prevented the adhesion of E. coli through the expulsion of chemotaxis. This study provides a theoretical basis and new thoughts for designing antibacterial adhesion strategies from the perspective of bacterial physiological regulation.

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Section: Methodsmentioning

confidence: 99%

Comparison of the Antiadhesion Activity and Mechanism between Different Chiral Molecules L-Borneol and D-Borneol against Escherichia coli

Wan,

Cai,

Gong

et al. 2024

ACS Food Sci. Technol.

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“…To obtain n ( z ), the number of trajectory points located at a given z was normalized by their trajectory length. For more details about n ( z ), please refer to our previous work . The range of n ( z ) from 0 to 50% for bacteria cells near the surface was defined as D r .…”

Section: Methodsmentioning

confidence: 99%

“…For more details about n(z), please refer to our previous work. 19 The range of n(z) from 0 to 50% for bacteria cells near the surface was defined as D r . The forward movement of the monoitrichate PAO1 occurred when its single flagellum rotated counterclockwise, while clockwise rotation caused the bacteria to move backward.…”

Section: Flow Chamber and Application Of Epmentioning

confidence: 99%

“…Our previous work showed reduced adhesion of Escherichia coli due to direct interruption of flagella rotation to inhibit the near-surface bacterial wobbling and surface collisions during the period of alternative electric fields. 19 Until now, most applications have adopted a long-term 20 and comparably high electric field intensity 21,22 surface polarization to inactivate microorganisms and prevent biofilm growth, which is not optimal for energy saving. An interesting question is whether the inhibition of bacterial adhesion can be sustained through the use of low-energy and short-duration EP.…”

Section: Introductionmentioning

confidence: 99%

“…To fill the abovementioned knowledge gap, a unique optical technique, digital holographic microscopy (DHM), allowing real-time observation of the 3D behavior of swimming bacteria, 19,26,27 was utilized to track the 3D motions of PAO1 during and after EP application. DHM has been used to study the impact of dead bacteria on the 3D movement of live bacteria and the influence of rough surfaces on human sperm migration.…”

Section: Introductionmentioning

confidence: 99%

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Adaptive Escape of Pseudomonas aeruginosa by Application of Low-Amplitude Electric Pulses

Feng,

Liu,

Bao

et al. 2024

Langmuir

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Marine antibiofouling using low-amplitude electric pulses (EP) is an energy-efficient and eco-friendly approach, but potential mechanisms for preventing biofouling remain unclear. In the present study, the 3D adhesion dynamics of a model microorganism�Pseudomonas aeruginosa (PAO1)�under lowamplitude cathodic EP were examined as a function of applying voltage and its duration (t d ). The results demonstrated that adhered bacteria escaped from the electrode surface even when EP was removed. The escaped bacteria ratio, induction period of escape, and duration of the detachment were influenced profoundly by EP amplitude but slightly by t d when t d ≥ 5 min. The acceleration of escaped PAO1 from the surface indicated that their flagellar motor was powered by EP. Particularly, EP enabled swimming bacteria to have adaptive motions that were sustainable and regulated by the gene rsmA. As a result, they had less accumulation near the surface. The propulsion of adhered bacteria and adaptive escape of swimming bacteria were enhanced in response to low-amplitude EP. Hence, low-amplitude and short-duration EP is promising for sustainable antibiofouling applications.

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Antifouling mechanism of natural product-based coatings investigated by digital holographic microscopy

Pan

Peng

Zhang

et al. 2021

Journal of Materials Science & Technology

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Alternating electric fields induce a period-dependent motion ofEscherichia coliin three-dimension near a conductive surface

Cited by 4 publications

References 36 publications

Comparison of the Antiadhesion Activity and Mechanism between Different Chiral Molecules L-Borneol and D-Borneol against Escherichia coli

Comparison of the Antiadhesion Activity and Mechanism between Different Chiral Molecules L-Borneol and D-Borneol against Escherichia coli

Adaptive Escape of Pseudomonas aeruginosa by Application of Low-Amplitude Electric Pulses

Antifouling mechanism of natural product-based coatings investigated by digital holographic microscopy

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