Alternation of the gut microbiota in irritable bowel syndrome: an integrated analysis based on multicenter amplicon sequencing data

Abstract

Background Gut dysbacteriosis has been reported as one of the etiologies for irritable bowel syndrome (IBS). However, the association between gut microbiota and IBS is still inconclusive. Method A paired-sample study was designed by retrieving original multicenter 16 s-rRNA data of IBS patients and healthy controls from the GMrepo database. The propensity score matching (PSM) algorithm was applied to reduce confounding bias. The differential analysis of microbiota composition was performed at different taxonomic levels. The co-occurrence network was established. Subgroup analysis was performed to identify specific microbial compositions in different IBS subtypes. Results A total of 1522 amplicon samples were initially enrolled. After PSM, 708 individuals (354 IBS and 354 healthy controls) were eligible for further analysis. A total of 1,160 genera were identified. We identified significantly changed taxa in IBS groups (IBS-enriched: the families Enterobacteriaceae, Moraxellaceae and Sphingobacteriaceae; the genera Streptococcus, Bacillus, Enterocloster, Sphingobacterium, Holdemania and Acinetobacter. IBS-depleted: the phyla Firmicutes, Euryarchaeota, Cyanobacteria, Acidobacteria and Lentisphaerae; the families Bifidobacteriaceae, Ruminococcaceae, Methanobacteriaceae and the other 25 families; the genera Faecalibacterium, Bifidobacterium and other 68 genera). The co-occurrence network identified three hub genera and six hub species (including Faecalibacterium prausnitzii) that may be involved in IBS pathophysiology. Strong positive interactions were identified among the Bifidobacterium longum, Bifidobacterium breve and Bifidobacterium adolescentis in the Bifidobacterium community. Conclusion This study provides quantitative analysis and visualization of the interaction between the gut microbiota and IBS. The identification of key species should be further validated to evaluate their causal relationships with the pathogenesis of IBS.

Profiling of the fecal microbiota and circulating microRNA-16 in IBS subjects with Blastocystis infection : a case–control study

“…The richness of Firmicutes in IBS patients compared to healthy controls is controversial. Although available evidence supports our results, which indicate higher abundance of Firmicutes in IBS patients [ 8 ], many studies have demonstrated lower richness of Firmicutes in IBS patients compared to control subjects [ 2 , 41 , 42 ]. This contentious trend was also observed for the abundance of Actinobacteria, and Faecalibacterium [ 2 , 8 , 43 , 44 ].…”

Multi-Omics Analysis of Gut Microbiota and Host Transcriptomics Reveal Dysregulated Immune Response and Metabolism in Young Adults with Irritable Bowel Syndrome

“…Previous studies confirm the role of Sutterella in small intestine edema and in the development and progress of IBD [ 26 , 27 ]. A multicenter data analysis with 1522 amplicon samples confirms that Holdemania is enriched among IBS subjects [ 28 ].…”

Gut microbiome and plasma metabolome alterations in myopic mice

“…Firmicutes, one of the major phyla in human gut microbiota, is essential for maintenance the integrity of the intestinal epithelial barrier, immune defenses against pathogenic microorganisms ( Jandhyala et al, 2015 ; Lv et al, 2023 ). Firmicutes depletion is involved in diseases with impaired intestinal barrier including irritable bowel syndrome ( Chen et al, 2023 ), colitis ( Boger-May et al, 2022 ) and Crohn’s disease ( Lv et al, 2023 ). On the other hand, Actinobacteria phylum contains numerous potential pathobionts that have the potential to cause intestinal inflammation ( Daniel et al, 2021 ).…”