Alternative Electron Sources for Cytochrome P450s Catalytic Cycle: Biosensing and Biosynthetic Application

Shumyantseva, Victoria V.; Koroleva, P. I.; Булко, Т. В.; Agafonova, Lyubov E.

doi:10.3390/pr11061801

Cited by 3 publications

(1 citation statement)

References 79 publications

(251 reference statements)

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“…With the pursuit of green, sustainable processes, the application of light or electricity to drive P450 catalysis may become mainstream. Combined with the advantages of biocatalysis and photoelectrocatalysis, the use of green energy for selective conversion has many advantages, including novel reactivity, high enantioselectivity, green synthesis, and high yield, which provide the basis for the application and development of P450s as bioreactors and biosensors [ 152 ]. Photo/electrically induced electron or energy transfer enables the synthesis method to complement the traditional dual electron transfer process for P450s or provide an orthogonal pathway for the development of new reactions.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Engineering Electron Transfer Pathway of Cytochrome P450s

He,

Liu,

2024

Molecules

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Cytochrome P450s (P450s), a superfamily of heme-containing enzymes, existed in animals, plants, and microorganisms. P450s can catalyze various regional and stereoselective oxidation reactions, which are widely used in natural product biosynthesis, drug metabolism, and biotechnology. In a typical catalytic cycle, P450s use redox proteins or domains to mediate electron transfer from NAD(P)H to heme iron. Therefore, the main factors determining the catalytic efficiency of P450s include not only the P450s themselves but also their redox-partners and electron transfer pathways. In this review, the electron transfer pathway engineering strategies of the P450s catalytic system are reviewed from four aspects: cofactor regeneration, selection of redox-partners, P450s and redox-partner engineering, and electrochemically or photochemically driven electron transfer.

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Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Engineering Electron Transfer Pathway of Cytochrome P450s

He,

Liu,

2024

Molecules

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Comparative Analysis of Bioelectrocatalytic Cytochrome P450 3A4 Systems

Koroleva,

Shumyantseva

2024

BMCRM

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This article describes the approaches developed by the authors with the aim to increase the efficiency of electro enzymatic reactions catalyzed by cytochrome P450 3A4. A comparative analysis of cytochrome P450 3A4 systems was carried out during the formation of the functional complexes hemoprotein-flavin nucleotides as low-molecular models of NAD(P)H-dependent cytochrome P450 reductase. The formation of a productive enzyme-substrate complex before the stage ofaccepting electrons from the modified electrode was studied from the electocatalytic viewpoint. Incorporation of the enzyme into nanopores of different nature on the electrode (2D-3D transition) was also studied. The results on the electrochemical reduction of bactosomes as the functionally active models of the microsomal monooxygenase system are also considered. The electrochemical and electrocatalytic parameters of cytochrome P450 3A4 were compared for different models of the electrocatalytic generation of metabolites.

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Biocatalysis: modern problems and applications

Varfolomeev,

Švedas,

Efremenko

et al. 2024

RUSS CHEM REV

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The review is devoted to analysis of recent advances, problems, and practical applications of enzymes in the framework of chemical enzymology and enzyme engineering. The achievements in the fundamental understanding of the molecular mechanisms of the catalytic cycle of enzymatic reactions are considered using quantum/molecular mechanics methods with supercomputer technologies and bioinformatics methods. The construction of protein biocatalysts with new properties is a fundamentally significant methodology of the bioengineering approach to solving practical problems, which is demonstrated by a number of examples. The increasing role of biocatalysis in medicine and biomedical research is illustrated by solving the problems of antibiotic synthesis and overcoming bacterial resistance to them, studying the mechanisms of neurodegenerative diseases and developing drugs to treat Alzheimer's disease, and investigation of biocatalytic processes of DNA repair and the role of mechanisms of functioning of heme peroxidases in the human body. The use of enzymes that destroy various endogenous and exogenous toxicants has been greatly developed in recent decades. The problems of using enzymes in therapy and drug delivery are analyzed. The fundamental role of enzymes in modern analysis and diagnosis is noted. The review considers a new trend in the development of bioanalytical methods using aptamers, multianalytical biochips, surface-enhanced Raman scattering systems, and bioelectroanalysis.<br> The bibliography includes 460 references.

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Alternative Electron Sources for Cytochrome P450s Catalytic Cycle: Biosensing and Biosynthetic Application

Cited by 3 publications

References 79 publications

Engineering Electron Transfer Pathway of Cytochrome P450s

Engineering Electron Transfer Pathway of Cytochrome P450s

Comparative Analysis of Bioelectrocatalytic Cytochrome P450 3A4 Systems

Biocatalysis: modern problems and applications

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