Alternative hydrogen sink pathways in hindgut fermentation

Demeyer, Daniël; Piattoni, Federica; Mbanzamihigo, Léonidas; Immig, Irmgard; Nollet, Leo M.L.

doi:10.1051/rnd:19970750

Cited by 3 publications

(1 citation statement)

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“…Hydrogen utilizing bacteria are necessary to prevent blocking of the fermentation reactions. It has been demonstrated that in the case of inhibition of methanogenesis, which are the main hydrogen utilizers, propionate fermentation increased (55). Lactate can be used as alternative hydrogen sink to enable glycolysis to continue, thereby stimulating propionate production (54,56).…”

Section: Discussionmentioning

confidence: 99%

Development of a Six-Stage Culture System for Simulating the Gastrointestinal Microbiota of Weaned Infants

Boever¹,

Wouters²,

Vermeirssen³

et al. 2001

Microbial Ecology in Health and Disease

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The development of a six-stage culture system simulating the microbial community present in the intestine of weaned infants is described. Plate counts of selected fecal indicator-organisms revealed high concentrations in the culture liquid of the simulator and these data paralleled those in infant feces. The metabolic patterns of the microbiota present in the model and the infant feces, compared by means of BIOLOG, revealed similar carbon utilization pro les. A genetic ngerprinting by means of Denaturing Gradient Gel Electrophoresis generated a more complex banding pattern in the culture system than in infant feces. The three vessels representing the colon showed similar DGGE patterns, indicating a high stability of the dominant microbial species. Yet, high variability in terms of the dominant bacteria was observed in infant stools. The fermentative capacity of the in ×itro system, characterized by the concentration of volatile fatty acids and ammonium, was in accordance with the in ×i×o data. The enhanced production of propionate in ×itro is probably due to the high concentration of fermentable sugars in the culture medium and hence, a high fermentation rate. The elevated concentration of propionate, as well as valerate and caproate in ×itro suggests conditions favoring Megasphaera sp. fermentation. The data concerning microbial diversity and activity warrants further exploration of the in ×itro model as a tool for studying the impact of newly developed infant formulas, functional foods and pharmaceuticals on the gastrointestinal microbiota of infants.

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Section: Discussionmentioning

confidence: 99%

Development of a Six-Stage Culture System for Simulating the Gastrointestinal Microbiota of Weaned Infants

Boever¹,

Wouters²,

Vermeirssen³

et al. 2001

Microbial Ecology in Health and Disease

View full text Add to dashboard Cite

show abstract

Development of a Six-Stage Culture System for Simulating the Gastrointestinal Microbiota of Weaned Infants

Boever¹,

Wouters²,

Vermeirssen³

et al. 2001

Microbial Ecology in Health & Disease

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Challenges in simulating the human gut for understanding the role of the microbiota in obesity

Aguirre

Venema

2017

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There is an elevated incidence of cases of obesity worldwide. Therefore, the development of strategies to tackle this condition is of vital importance. This review focuses on the necessity of optimising in vitro systems to model human colonic fermentation in obese subjects. This may allow to increase the resolution and the physiological relevance of the information obtained from this type of studies when evaluating the potential role that the human gut microbiota plays in obesity. In light of the parameters that are currently used for the in vitro simulation of the human gut (which are mostly based on information derived from healthy subjects) and the possible difference with an obese condition, we propose to revise and improve specific standard operating procedures.

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Alternative hydrogen sink pathways in hindgut fermentation

Cited by 3 publications

References 1 publication

Development of a Six-Stage Culture System for Simulating the Gastrointestinal Microbiota of Weaned Infants

Development of a Six-Stage Culture System for Simulating the Gastrointestinal Microbiota of Weaned Infants

Development of a Six-Stage Culture System for Simulating the Gastrointestinal Microbiota of Weaned Infants

Challenges in simulating the human gut for understanding the role of the microbiota in obesity

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