Alternative method for measuring beeswax content in propolis from the Netherlands

Hogendoorn, E.A.; Sommeijer, M. J.; Vredenbregt, M.J.

doi:10.2478/jas-2013-0019

Cited by 17 publications

(15 citation statements)

References 32 publications

(43 reference statements)

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“…While the RP was stored at −20ºC, EEP and WEP were stored at +4 ºC in a dark glass tube until the beginning of the trial. The RP used in this study was analysed for dry matter, ash, ether extracts (AOAC, 1990), dry residue (Hogendoorn et al, 2013), waxes, ethanol-insoluble residues (Cunha et al, 2004), and phenols and flavonoids (Kartal et al, 2002). Active compounds of EEP and WEP (Table 2) were analysed with gas chromatography-mass spectrometry, as explained by Ramnath et al (2015).…”

Section: Methodsmentioning

confidence: 99%

Effects of raw propolis or water and ethanol extracts of propolis on performance, immune system, and some blood parameters of broiler breeders

Şahin

Öztürk

2018

R. Bras. Zootec.

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The objective of this study was to determine the effects of raw bee propolis and water or ethanol extract of propolis on growth performance, some blood parameters, and immunoglobulins in 15-20-week-old Ross-308 broiler breeders. The birds in the control were fed a diet without propolis, whereas the birds in the treatment groups were fed diets with raw propolis (RP), water (WEP), and ethanol (EEP) extract of propolis at the level of 1200, 400, and 400 ppm, respectively. Raw propolis and propolis extracts did not affect body weight gain, feed intake, feed conversion ratio, and some blood parameters, such as aspartate aminotransferase, alanine aminotransferase, total antioxidant status, triglyceride, and phosphorus. Birds fed EEP and those in control group had a lower IgA value compared with birds fed RP. Birds fed RP had higher IgM level than those of the other groups, and birds fed EEP had lower IgM level than those of control and RP-fed group. The IgY value of breeders fed EEP was higher than those of the other treatment birds, whereas that of WEP-fed birds was higher than those of control and RP treatment. The antibody levels of Anti-Newcastle disease virus and anti-infectious bursal disease virus were higher in EEP and RP-fed groups than those in the control and WEP-fed groups. The WEP decreased total oxidant status value compared with the control and RP treatments, whereas EEP and WEP increased plasma total protein and calcium contents compared with the control. The EEP increased plasma albumin content compared with RP. The addition of propolis extracts, especially WEP and EEP, to diet improves immunity and antioxidant activity, as well as enhances Ca absorption of broiler breeders.

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Section: Methodsmentioning

confidence: 99%

Effects of raw propolis or water and ethanol extracts of propolis on performance, immune system, and some blood parameters of broiler breeders

Şahin

Öztürk

2018

R. Bras. Zootec.

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“…Wax content measurement based on differences in specific density. An alternative procedure for measuring the wax content of propolis has been described by Hogendoorn, Sommeijer, and Vredenbregt (2013).…”

Section: Wax Contentmentioning

confidence: 99%

Standard methods forApis melliferapropolis research

Bankova

Bertelli

Borba

et al. 2016

Journal of Apicultural Research

204

144

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Propolis is one of the most fascinating honey bee (Apis mellifera L.) products. It is a plant derived product that bees\ud produce from resins that they collect from different plant organs and with which they mix beeswax. Propolis is a building\ud material and a protective agent in the bee hive. It also plays an important role in honey bee social immunity, and is\ud widely used by humans as an ingredient of nutraceuticals, over-the-counter preparations and cosmetics. Its chemical\ud composition varies by geographic location, climatic zone and local flora. The understanding of the chemical diversity of\ud propolis is very important in propolis research. In this manuscript, we give an overview of the available methods for\ud studying propolis in different aspects: propolis in the bee colony; chemical composition and plant sources of propolis;\ud biological activity of propolis with respect to bees and humans; and approaches for standardization and quality control\ud for the purposes of industrial application

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“…These methods are specific to each compound and time-consuming; and they need important amounts of polluting solvents, while NIR technology is known for being a multiparametric, rapid, and non-destructive technique. It has been used in the characterisation of Mexican propolis with FTIR UV-Vis techniques [ 28 ], in the determination of chrysin and galangin in Chinese propolis [ 29 ], in the detection of the adulteration of propolis with Poplar balata [ 30 ], in the identification of beeswax in this product [ 31 ], in the assessment of the mineral composition [ 32 ], and in more recent applications such as the estimation ofcaffeic acid phenylethyl ester or CAPE [ 33 ] or the determination of pest control substances in propolis [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

Flavonoid and Antioxidant Capacity of Propolis Prediction Using Near Infrared Spectroscopy

Betances-Salcedo

Revilla

Quintana

et al. 2017

Sensors

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The use of propolis as a dietary supplement or as an ingredient in different food products is increasing, due to its antioxidant and bactericidal properties. These nutritional properties directly depend on its phenolic composition. For this reason, this study analysed the total contents of flavones and flavonols, flavanones and dihydroflavonols, and the antioxidant capacity by using the methods of ABTS and linoleic acid/β-carotene in 99 samples of propolis from Spain and Chile. A rapid method was developed for quantifying these parameters in raw propolis using near infrared (NIR) spectroscopy with a remote reflectance fibre-optic probe applied directly to the ground-up sample. The models developed allow for the determination of the total flavones and flavonols (0–183 mg quercetin/g propolis and 0–72 mg rutin/g propolis), of the total flavanones and dihydroflavonols (9–109 mg pinocembrin/g propolis extract), and of its antioxidant capacity by the ABTS method based on the reduction of the 2.2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation(0–3212.6 nmol Trolox/mg of propolis) and of linoleic acid/β-carotene (22–86% inhibition). The NIR spectroscopy models were applied in external validation to different samples of the calibration group, which led to the conclusion that the methods developed provide significantly identical data to the initial chemical data of reference.

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Alternative method for measuring beeswax content in propolis from the Netherlands

Cited by 17 publications

References 32 publications

Effects of raw propolis or water and ethanol extracts of propolis on performance, immune system, and some blood parameters of broiler breeders

Effects of raw propolis or water and ethanol extracts of propolis on performance, immune system, and some blood parameters of broiler breeders

Standard methods forApis melliferapropolis research

Flavonoid and Antioxidant Capacity of Propolis Prediction Using Near Infrared Spectroscopy

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