Alternative methods for RuBisCO extraction from sugar beet waste: A comparative approach of ultrasound and high voltage electrical discharge

Dukić, Josipa; Košpić, Karla; Kelava, Vanja; Mavrić, Renata; Nutrizio, Marinela; Balen, Biljana; Butorac, Ana; Halil Öztop, Mecit; Režek Jambrak, Anet

doi:10.1016/j.ultsonch.2023.106535

Cited by 6 publications

(6 citation statements)

References 89 publications

(100 reference statements)

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“…As demonstrated in Table 2 and Table 4 , despite the extraction optimization, the obtained PEY values denote the challenges when extracting RuBisCo and other green leaf proteins, as their location within the leaf matrix in combination with the high moisture content of leaves limits the obtention of high protein yields and pure protein isolates, as well as their industrial exploitation and applicability [ 29 , 40 ].…”

Section: Resultsmentioning

confidence: 99%

“…Apart from RuBisCo, one of the most common protein types found in the chloroplasts of leaves are chlorophyll light-binding complexes [ 40 ]. Furthermore, the transformation of chlorophyll into pheophytin by acidic conditions results in an olive-green-colored product.…”

Section: Resultsmentioning

confidence: 99%

“…Those changes can be prevented by alkaline conditions in which chlorophylls are maintained [ 44 ]. In addition, the integration of ultrasound might be beneficial for extracting not only chlorophyll but also carotenoids, which can also be found in the chloroplasts of duckweed to support photosynthesis [ 40 , 45 , 46 ].…”

Section: Resultsmentioning

confidence: 99%

“…A protein extract from Wolffia globosa obtained through ultrasound-assisted extraction (120 kHz, 15 min) had five main bands at 25, 30, 45, 63, and 100 kDa [ 34 ]; these values also corresponded with the bands reported at 20, 25, 30.6, 45.6, and 61.5 kDa for enzymatically prepared protein hydrolysates from the same duckweed species [ 39 ]. The bands at 14 and 56 kDa can be attributed to subunits of RuBisCo [ 40 , 51 ], which have been fully observed in Spirodela polyrhiza [ 50 ] and in Lemna minor as an abundant band between 50 and 60 kDa [ 52 ].…”

Section: Resultsmentioning

confidence: 99%

“…Based on the SDS-PAGE result, we observed a beneficial effect from ultrasound on the extraction of protein fractions that are difficult to extract, as already reported for RuBisCo, which has been reported as the main protein in duckweed, regardless of its extraction limitations due to its location within the plant matrix. Hence, higher RuBisCo yields can be obtained due to the enhanced mass transfer produced by ultrasound cavitation [ 29 , 40 ]. This corresponds with the presence of a more intense band at 50 kDa in the ultrasound sample, as well as a unique band at 62 KDa.…”

Section: Resultsmentioning

confidence: 99%

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The Effect of Ultrasound on the Extraction and Functionality of Proteins from Duckweed (Lemna minor)

Mirón-Mérida,

Soria-Hernández,

Richards-Chávez

et al. 2024

Molecules

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The inclusion of protein in the regular human diet is important for the prevention of several chronic diseases. In the search for novel alternative protein sources, plant-based proteins are widely explored from a sustainable and ecological point of view. Duckweed (Lemna minor), also known as water lentil, is an aquatic plant with potential applications for human consumption due to its protein content and carbohydrate contents. Among all the conventional and novel protein extraction methods, the utilization of ultrasound has attracted the attention of scientists because of its effects on improving protein extraction and its functionalities. In this work, a Box–Behnken experimental design was proposed to optimize the alkaline extraction of protein from duckweed. In addition, an exploration of the effects of ultrasound on the morphological, structural, and functional properties of the extracted protein was also addressed. The optimal extraction parameters were a pH of 11.5 and an ultrasound amplitude and processing time of 60% and 20 min, respectively. These process conditions doubled the protein content extracted in comparison to the value from the initial duckweed sample. Furthermore, the application of ultrasound during the extraction of protein generated changes in the FTIR spectra, color, and structure of the duckweed protein, which resulted in improvements in its solubility, emulsifying properties, and foaming capacity.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%