Alternative myelomonocytic differentiation of HL-60 reflects dual prospective potency of promyelocytes in human

Identification of Cytoplasmic Actin as an Abundant Glutaminyl Substrate for Tissue Transglutaminase in HL-60 and U937 Cells Undergoing Apoptosis

“…3), although HL-60 showed consequently weaker labeling intensity. This is in agreement with the weaker and more limited induction of nonspecific esterase in these cells (25) as compared with U937 cells (34).…”

“…Both type of cells express high levels of transglutaminase when undergo apoptosis following differentiation (22)(23)(24). The maturated, macrophage-like phenotypes adhere to glass and contain much more nonspecific esterase activity as compared with granulocytic forms (25,26). We examined the glutaminyl substrate proteins of tissue TGase in apoptotic cell cultures by analyzing the incorporation of the novel haptenized derivative of lysine into proteins.…”

Protein transamidation by transglutaminase 2 in cells: a disputed Ca2+‐dependent action of a multifunctional protein

“…The intracellular concentration of endogenous polyamines (such as putrescine, spermidine and spermine) can affect apparent in situ TG2 activity. 5′‐Biotinamido‐pentylamine [67,68] and monodansyl‐cadaverine [69], the artificial substrates many investigators use to incorporate haptenic groups into substrate proteins or measure in situ activity, have undetermined membrane permeability, which may vary between cell types or can be subject to change upon treatment. This is something often not factored into interpretations of data, such as in [70].…”

Urokinase plasminogen activator receptor (CD87) expression of tumor-associated macrophages in ductal carcinoma in situ, breast cancer, and resident macrophages of normal breast tissue

“…Cells were phenotyped by using a panel of antibodies: Ki-M2 [20], Ki-M5 [21], Ki-M6 [22], Ki-M7 [23], Ki-M8 [24], RM 3/1 [25], 27E10 [26], 25F9 [27], G16/1 [28], anti-CD11b [29], (all from Dianova, Hamburg, Germany), EMB11 [30], PG-M1 [31], KP1, MAC387, anti-CD45RB (leukocyte common antigen) (lca), anti-CD31, anti-CD18 (MHM23) (all from DAKO, Hamburg, Germany), Leu-M3 [32] (Becton-Dickinson, Heidelberg, Germany), anti-human fibroblast antigen (Dianova, Hamburg, Germany), anti-pan-cytokeratin (ck) (ProGen, Heidelberg, Germany), and anti-uPAR [33,34] (no. 3936, American Diagnostica, Greenwich, CT).…”

“…The confocal aperture was set at 50 µm. A series of optical sections (14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25) was collected at incremental steps of 0.3 µm. Unprocessed optical sections with an image size of 512 ϫ 512 pixels and pixel size of 0.2 µm were obtained.…”