Alternative oxidase (AOX) and phenolic metabolism in methyl jasmonate-treated hairy root cultures of Daucus carota L.

Sircar, Debabrata; Cardoso, Hélia; Mukherjee, Chiranjit; Mitra, Adinpunya; Arnholdt‐Schmitt, Birgit

doi:10.1016/j.jplph.2011.11.019

Cited by 37 publications

(23 citation statements)

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“…Phenolic extracts (and as well as total carotenoids extracts) of MeJA-treated plants showed a higher scavenging activity of hydroxyl and superoxide radicals (Divya et al 2014). The time course study of phenols and flavonoids accumulation in Daucus carota (Sircar et al 2012) was similar to our results. Moreover, the MeJA treatment enhanced the anthocyanin production, effective antioxidants with dietary and pharmaceutical activities, in various plant species and tissues (Ram et al 2013;Tassoni et al 2012).…”

Section: Discussionsupporting

confidence: 88%

“…In addition, MeJAenhanced accumulation of soluble and wall-bound phenols (e.g. hydroxybenzoic acid, derivates of cinnamic and hydoxycinnamic acid) displayed a time dependent (Sircar et al 2012) or a concentration dependent behaviour (Sahu et al 2013). Moreover, coumarins with a higher amount of hydroxyl groups express better scavenging properties than unhydroxylated coumarins (Lin et al 2008).…”

Section: Discussionmentioning

confidence: 99%

“…The enhanced elicitation of plant secondary metabolites ensures the plant's survival, persistence and competitiveness. For example, exogenous MeJA application dramatically enhances not only the production of medically or dietary interesting metabolites, such as phenols (Ahn et al 2014;Sircar et al 2012), terpenoids (Wang et al 2010;Onrubia et al 2013) or anthocyanins (Ram et al 2013), but also other secondary metabolites accompanied with disease resistance, such as polyamines (Cao et al 2014). The expression of corresponding genes and the activity of enzymes has also been demonstrated that MeJA treatment triggers defence-related reactions in a variety of experimental plant systems, such as intact plants, detached plant parts, cells suspension cultures or protoplasts.…”

Section: Introductionmentioning

confidence: 99%

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Dynamics of accumulation of coumarin-related compounds in leaves of Matricaria chamomilla after methyl jasmonate elicitation

et al. 2015

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The effect of methyl jasmonate (MeJA) treatment on diploid and tetraploid plants of Matricaria chamomilla and the changes of the main physiological parameters and secondary metabolites content was studied. Leaf rosettes from 7-week-old plants were harvested at four different time points (24, 48, 72, 96 h) after foliar application of 0.4 mM MeJA. The treatment led to a moderate biomass accumulation accompanied by an accumulation of photosynthetic pigments and decrease in the total soluble proteins. The content of hydrogen peroxide and superoxide radical was most elevated at 24 and 72 h after the treatment. Although the values of stress parameters were higher in tetraploid plants (such as in control so in treated plants), their total increase after the MeJA application was similar. The absolute level of total soluble phenols and flavonoids in the controls was similar in both cultivars and their content was enhanced in 24-and 48-h variants, more rapidly in diploid plants. MeJA has been found to trigger different responses of the secondary metabolites accumulation. Almost all studied metabolites were higher in control plants in the tetraploid cultivar, but their biosynthesis was more stimulated in diploids. Significant changes, mainly increasing trends with a maximum between 24 and 48 h after elicitation, in the content of free coumarin herniarin and its glycosidic precursors (Z)-and (E)-2-b-D-glucopyranosyloxy-4-methoxycinnamic acid were observed. The umbelliferone level increased over time. The maximum values for chlorogenic acid and 1,5-dicaffeoylquinic acid were at 24-h and between the 24-72 h time points. Among dicycloethers, the amounts of (E)-form decreased with time, and for (Z)-form an opposite trend was observed, with a maximum of 96 h for diploid and 24 h for tetraploid after elicitation.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dynamics of accumulation of coumarin-related compounds in leaves of Matricaria chamomilla after methyl jasmonate elicitation

et al. 2015

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“…In the present study, we sought to demonstrate the potential antiparasite agent target of the AP in this ciliate. In contrast, in the same growth phase and under conditions of low oxygen availability, the ciliates utilized AMA-resistant respiration, which is completely inhibited by PG, a known inhibitor of the AOX (Sircar et al 2012), thus confirming the existence of an alternative AOX activity in P. dicentrarchi. In the present study, the ciliates grown at the early stages of exponential growth in a normoxic environment respired by the conventional CP, as shown by the complete inhibition of O 2 consumption by AMA.…”

Section: Discussionmentioning

confidence: 76%

“…This phenolic antioxidant is generally recognized as safe (GRAS) and has been extensively used in food since 1948 to protect fats, oils and fat-containing food from the rancidity resulting from the formation of peroxides. PG is a well-known inhibitor of lipoxygenase, a cyanideresistant dioxygenase found in most plant tissues (Parrish and Leopold, 1978;Peterman and Siedow, 1983), and it is also an inhibitor of alternative oxidase (AOX) (Siedow and Girvin, 1980;Costa et al 2010;Sircar et al 2012). PG is a well-known inhibitor of lipoxygenase, a cyanideresistant dioxygenase found in most plant tissues (Parrish and Leopold, 1978;Peterman and Siedow, 1983), and it is also an inhibitor of alternative oxidase (AOX) (Siedow and Girvin, 1980;Costa et al 2010;Sircar et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Alternative oxidase inhibitors as antiparasitic agents against scuticociliatosis

2014

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Philasterides dicentrarchi causes a severe disease in turbot, and at present there are no drugs available to treat infected fish. We have previously demonstrated that, in addition to the classical respiratory pathway, P. dicentrarchi possesses an alternative mitochondrial respiratory pathway that is cyanide-insensitive and salicylhydroxamic acid (SHAM)-sensitive. In this study, we found that during the initial phase of growth in normoxia, ciliate respiration is sensitive to the natural polyphenol resveratrol (RESV) and to Antimycin A (AMA). However, under hypoxic conditions, the parasite utilizes AMA-insensitive respiration, which is completely inhibited by RESV and by the antioxidant propyl gallate (PG), an alternative oxidase (AOX) inhibitor. PG caused significantly dose-dependent inhibition of the in vitro growth of the parasite under normoxia and hypoxia and an over-expression of heat shock proteins of the Hsp70 subfamily. RESV and PG may affect the protective role of the AOX against mitochondrial oxidative stress, leading to an impaired mitochondrial membrane potential and mitochondrial dysfunction, which the parasite attempts to neutralize by increasing the expression of Hsp70. In view of the antiparasitic effects induced by AOX inhibitors and the absence of AOX in their host, this enzyme constitutes a potential target for the development of new drugs against scuticociliatosis.

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Taxonomic distribution of alternative oxidase in plants

McDonald

2015

Alternative Respiratory Pathways in Higher Plants

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Alternative oxidase (AOX) and phenolic metabolism in methyl jasmonate-treated hairy root cultures of Daucus carota L.

Cited by 37 publications

References 58 publications

Dynamics of accumulation of coumarin-related compounds in leaves of Matricaria chamomilla after methyl jasmonate elicitation

Dynamics of accumulation of coumarin-related compounds in leaves of Matricaria chamomilla after methyl jasmonate elicitation

Alternative oxidase inhibitors as antiparasitic agents against scuticociliatosis

Taxonomic distribution of alternative oxidase in plants

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