Alternative Pathways of Carbohydrate Utilization in Pseudomonads

Lessie, T G; Phibbs, Paul V.

doi:10.1146/annurev.mi.38.100184.002043

Cited by 310 publications

(157 citation statements)

References 47 publications

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“…In the Entner-Doudoroff pathway, glucose is degraded through the key intermediate 6-phosphogluconate to glyceraldehyde-3-phosphate and pyruvate. The initial steps in the conversion of glucose to 6-phosphogluconate are the subsequent oxidation to gluconate and 2-keto-gluconate (32). We found a TAT-dependent signal sequence in the gene product of PA2264, which encodes a putative sugar dehydrogenase and is located in a cluster of genes implied in the utilization of 2-keto-gluconate (33).…”

Section: Twin-arginine Motif and Secretion Of Overexpressed Phospholimentioning

confidence: 79%

Effects of the twin-arginine translocase on secretion of virulence factors, stress response, and pathogenesis

Ochsner

Snyder

Vasil

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

189

226

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A novel secretion pathway originally found in plants has recently been discovered in bacteria and termed TAT, for ''twin-arginine translocation,'' with respect to the presence of an Arg-Arg motif in the signal sequence of TAT-secreted products. However, it is unknown whether the TAT system contributes in any way to virulence through the secretion of factors associated with pathogenesis or stress response. We found that the opportunistic pathogen Pseudomonas aeruginosa produces several virulence factors that depend on the TAT system for proper export to the periplasm, outer membrane, or extracellular milieu. We identified at least 18 TAT substrates of P. aeruginosa and characterized the pleiotropic phenotypes of a tatC deletion mutant. The TAT system proved essential for the export of phospholipases, proteins involved in pyoverdine-mediated ironuptake, anaerobic respiration, osmotic stress defense, motility, and biofilm formation. Because all these traits have been associated with virulence, we studied the role of TAT in a rat lung model. A tatC mutant did not cause the typical multifocal pulmonary abscesses and did not evoke a heavy inflammatory host response compared with wild type, indicating that tatC mutant cells are attenuated for virulence. Because the TAT apparatus is well conserved among important bacterial pathogens yet absent in mammalian cells, it represents a potential target for novel antimicrobial compounds.

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Section: Twin-arginine Motif and Secretion Of Overexpressed Phospholimentioning

confidence: 79%

Effects of the twin-arginine translocase on secretion of virulence factors, stress response, and pathogenesis

Ochsner

Snyder

Vasil

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

189

226

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“…Pseudomonas species lack the 6-phosphofructokinase gene required for glycolysis, and glucose is converted to glyceraldehyde-3-phosphate (GAP) and pyruvate (Pyr) via the Entner-Doudoroff (ED) pathway. In addition, several studies have already shown that fructose is mainly metabolized through the ED route by converting fructose-1,6-bisphosphate (FBP) to fructose-6-phosphate (F6P) and then to glucose-6-phosphate (G6P) (1,6) (Fig. 1).…”

Section: Importancementioning

confidence: 99%

Identification of a New Phosphatase Enzyme Potentially Involved in the Sugar Phosphate Stress Response in Pseudomonas fluorescens

Maleki

Hrudíková

Zotchev

et al. 2017

Appl Environ Microbiol

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The alginate-producing bacterium Pseudomonas fluorescens utilizes the Entner-Doudoroff (ED) and pentose phosphate (PP) pathways to metabolize fructose, since the upper part of its Embden-Meyerhof-Parnas pathway is defective. Our previous study indicated that perturbation of the central carbon metabolism by diminishing glucose-6-phosphate dehydrogenase activity could lead to sugar phosphate stress when P. fluorescens was cultivated on fructose. In the present study, we demonstrate that PFLU2693, annotated as a haloacid dehalogenase-like enzyme, is a new sugar phosphate phosphatase, now designated Spp, which is able to dephosphorylate a range of phosphate substrates, including glucose 6-phosphate and fructose 6-phosphate, in vitro. The effect of spp overexpression on growth and alginate production was investigated using both the wild type and several mutant strains. The results obtained suggested that sugar phosphate accumulation caused diminished growth in some of the mutant strains, since this was partially relieved by spp overexpression. On the other hand, overexpression of spp in fructose-grown alginate-producing strains negatively affected both growth and alginate production. The latter implies that Spp dephosphorylates the sugar phosphates, thus depleting the pool of these important metabolites. Deletion of the spp gene did not affect growth of the wildtype strain on fructose, but the gene could not be deleted in the alginate-producing strain. This indicates that Spp is essential for relieving the cells of sugar phosphate stress in P. fluorescens actively producing alginate.IMPORTANCE In enteric bacteria, the sugar phosphate phosphatase YigL is known to play an important role in combating stress caused by sugar phosphate accumulation. In this study, we identified a sugar phosphate phosphatase, designated Spp, in Pseudomonas fluorescens. Spp utilizes glucose 6-phosphate, fructose 6-phosphate, and ribose 5-phosphate as substrates, and overexpression of the gene had a positive effect on growth in P. fluorescens mutants experiencing sugar phosphate stress. The gene was localized downstream of gnd and zwf-2, which encode enzymes involved in the pentose phosphate and Entner-Doudoroff pathways. Genes encoding Spp homologues were identified in similar genetic contexts in some bacteria belonging to several phylogenetically diverse families, suggesting similar functions.

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“…In contrast to Enterobacteria, the Pseudomonas EM glycolytic pathway is nonfunctional due to the absence of 6-phosphofructokinase (7). Degradation of glucose proceeds ultimately via the ED pathway, where 6-phosphogluconate and KDPG are key intermediates.…”

Section: Bacteria Exploit Multiple Mechanisms For Controlling Centralmentioning

confidence: 99%

Control of Proteobacterial Central Carbon Metabolism by the HexR Transcriptional Regulator

Leyn

Zheng

et al. 2011

Journal of Biological Chemistry

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Background: Different regulatory strategies are utilized by bacteria to control central carbohydrate metabolism. Results: Transcriptional factor HexR is a global regulator of the central carbohydrate metabolism genes in various groups of proteobacterial genomes. Conclusion: HexR in Shewanella is a repressor/activator of the glycolytic/gluconeogenic genes. Significance: Integration of the comparative genomics and experimental approaches is efficient for reconstruction of transcriptional regulons in bacteria.

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Alternative Pathways of Carbohydrate Utilization in Pseudomonads

Cited by 310 publications

References 47 publications

Effects of the twin-arginine translocase on secretion of virulence factors, stress response, and pathogenesis

Effects of the twin-arginine translocase on secretion of virulence factors, stress response, and pathogenesis

Identification of a New Phosphatase Enzyme Potentially Involved in the Sugar Phosphate Stress Response in Pseudomonas fluorescens

Control of Proteobacterial Central Carbon Metabolism by the HexR Transcriptional Regulator

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