Alternative patterns of partial embryo compaction: prevalence, morphokinetic history and possible implications

Lagalla, Cristina; Coticchio, Giovanni; Sciajno, Raffaella; Tarozzi, Nicoletta; Zacà, Carlotta; Borini, Andrea

doi:10.1016/j.rbmo.2019.11.011

Cited by 37 publications

(26 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Handarson and colleagues described by time-lapse sequence imaging the inter-nalization/reabsorption of cellular fragments into neighboring blastomeres, while others disappear, leaving behind only debris [5]. A more recent suggestion is that an embryo can be capable of excluding any unwanted cell and/or cellular fragment from the remaining viable cells during the morula-to-blastocyst transition [14] (Figure 3). The complete exclusion of fragments or entire blastomeres can be observed in compacted embryos, morulae and blastocysts (Figure 4).…”

Section: • Timing Of Cytoplasmic Fragment Formationmentioning

confidence: 99%

“…Thanks to the use of TLM that allows a deeper observation of the events underling embryo development, blastomere exclusion has been observed both in morulae and blastocysts of several mammalian species (i.e., humans, rhesus macaques, cattle, and mice) [21][22][23][24][25][26][27][28][29] (Figure 3). Blastomeres could be excluded during the first phases of embryo compaction, while others could be extruded from the compacted morula after a transient involvement during this process [14]. Lagalla et al retrospectively evaluated 791 embryos obtained in 145 ART cycles by time-lapse morphokinetics analysis.…”

Section: Extruded Blastomeresmentioning

confidence: 99%

See 1 more Smart Citation

Cellular and Molecular Nature of Fragmentation of Human Embryos

Cecchele

Cermisoni

Giacomini

et al. 2022

IJMS

View full text Add to dashboard Cite

Embryo fragmentation represents a phenomenon generally characterized by the presence of membrane-bound extracellular cytoplasm into the perivitelline space. Recent evidence supports the cellular and molecular heterogeneity of embryo fragments. In this narrative review, we described the different embryo fragment-like cellular structures in their morphology, molecular content, and supposed function and have reported the proposed theories on their origin over the years. We identified articles related to characterization of embryo fragmentation with a specific literature search string. The occurrence of embryo fragmentation has been related to various mechanisms, of which the most studied are apoptotic cell death, membrane compartmentalization of altered DNA, cytoskeletal disorders, and vesicle formation. These phenomena are thought to result in the extrusion of entire blastomeres, release of apoptotic bodies and other vesicles, and micronuclei formation. Different patterns of fragmentation may have different etiologies and effects on embryo competence. Removal of fragments from the embryo before embryo transfer with the aim to improve implantation potential should be reconsidered on the basis of the present observations.

show abstract

Section: • Timing Of Cytoplasmic Fragment Formationmentioning

confidence: 99%

Section: Extruded Blastomeresmentioning

confidence: 99%

Cellular and Molecular Nature of Fragmentation of Human Embryos

Cecchele

Cermisoni

Giacomini

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…However, the determination of embryonic development 72 h after insemination might be more appropriate for the "compacting morula" rather than the "morula" in mice. Although previous studies have observed compaction and subsequent embryonic development using time-lapse technology in human embryos, these focused on vacuolization in blastomeres [24], timing of morula formation [22,23,25,26], and patterns of compaction with or without extruded blastomeres [22,27]. There have been, to our knowledge, no previous studies involving the observation of a temporary loosening of compaction during the formation of morula beginning with the initiation of compaction in human embryos [44].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the automatic capture of time-lapse images would provide useful information for embryo selection for subsequent embryo transfer. Previous studies have investigated pronuclear formation [15,16], timing of cleavage [16,17], fragmentation [18][19][20][21], compaction [22][23][24][25][26][27], blastocoel formation [26,28], and the shape of the inner cell mass [29] in human zygotes/embryos using a time-lapse monitoring system. Embryos that cleave early at the first cleavage have a higher potential for subsequent pre-and post-implantation development [17,[30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Morphokinetic Analysis of Preimplantation Development of Mouse Oocytes Fertilised in Vitro Using a Non-humidifying Incubator With Time-lapse Cinematography (CCM-iBIS)

Watanabe¹,

Ito²,

Shintome³

et al. 2021

Preprint

View full text Add to dashboard Cite

Preimplantation development of mouse oocytes fertilised in vitro was assessed in a non-humidified incubator with time-lapse cinematography (CCM-iBIS). The developmental rates of embryos to the 4-cell and blastocyst stages under 5% CO2, 5% O2, and 90% N2 in CCM-iBIS were significantly higher than those under 5% CO2 in air in CCM-iBIS and a conventional CO2 incubator (CPO2-2301). The developmental speed of embryos was much faster in those cultured under lower oxygen tension in CCM-iBIS than in higher oxygen tension in CCM-iBIS and CPO2-2301. Embryonic development was much faster and more synchronised under lower oxygen tension. Non-humidified culture did not affect the development of the embryos. Mouse embryos cultured at lower oxygen tension reached 2-cell at 17 h, 3-cell at 39 h, 4-cell at 40 h, initiation of compaction at 58 h, morula at 70 h, and blastocyst at 82 h after insemination on average. Although compaction partially unravelled with cell division in compacting embryos, it appeared to complete depending on the timing of observation. Observation at a conventional 24-h interval 13 likely misjudges the developmental rate to morula. Determination of embryonic development 72 h after insemination may be more appropriate for “compacting morula” rather than “morula” in mice.

show abstract

“…Time-lapse observations have also advanced our understanding of the morphological mechanisms of fertilization, development, and behavior of early embryos during the preimplantation period. Previous studies with time-lapse monitoring have investigated pronuclear formation [ 4 , 5 ], timing of cleavage [ 5 , 6 , 7 , 8 , 9 ], fragmentation [ 10 , 11 , 12 , 13 ], compaction [ 14 , 15 , 16 , 17 , 18 , 19 ], blastocoel formation [ 18 , 20 ], and shape of the inner cell mass [ 21 ]. Furthermore, culturing human embryos in an incubator with a time-lapse monitoring system may improve development up to the blastocyst stage and pregnancy outcomes, compared to the use of a conventional incubator [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of oxygen tension and humidity on the preimplantation development of mouse embryos produced by <i>in vitro</i> fertilization: analysis using a non-humidifying incubator with time-lapse cinematography

et al. 2022

View full text Add to dashboard Cite

To examine the effects of oxygen tension and humidity on early embryonic development, the preimplantation development of mouse embryos produced by in vitro fertilization was assessed by time-lapse cinematography to evaluate morphokinetic development with higher precision. Zygotes were produced from spermatozoa and oocytes from ICR mice and cultured in KSOM under low or high oxygen tension in a non-humidified incubator with time-lapse cinematography (CCM-iBIS). The developmental rates of embryos to the 4-cell and blastocyst stages under lower oxygen tension in CCM-iBIS were significantly higher than those under higher oxygen tension in CCM-iBIS. Ninety-six hours after insemination, a large number of embryos cultured under low oxygen tension developed to the hatching blastocyst stage. Embryonic development was more synchronized under lower oxygen tension. Non-humidified cultures did not affect embryonic development. On average, mouse embryos cultured at lower oxygen tension reached 2-cell at 18 h, 3-cell at 39 h, 4-cell at 40 h, initiation of compaction at 58 h, morula at 69 h, and blastocyst at 82 h after insemination. In conclusion, lower oxygen tension better supports preimplantation development of mouse embryos fertilized in vitro , and non-humidified culture conditions do not influence the embryonic development in vitro .

show abstract

Alternative patterns of partial embryo compaction: prevalence, morphokinetic history and possible implications

Cited by 37 publications

References 25 publications

Cellular and Molecular Nature of Fragmentation of Human Embryos

Cellular and Molecular Nature of Fragmentation of Human Embryos

Morphokinetic Analysis of Preimplantation Development of Mouse Oocytes Fertilised in Vitro Using a Non-humidifying Incubator With Time-lapse Cinematography (CCM-iBIS)

Effects of oxygen tension and humidity on the preimplantation development of mouse embryos produced by <i>in vitro</i> fertilization: analysis using a non-humidifying incubator with time-lapse cinematography

Contact Info

Product

Resources

About