Alternative splicing converts the G-protein coupled follitropin receptor gene into a growth factor type I receptor: Implications for pleiotropic actions of the hormone

Sairam, M.R.; Jiang, L.G.; Yarney, T.A.; Khan, Haseena

doi:10.1002/(sici)1098-2795(199712)48:4<471::aid-mrd7>3.0.co;2-n

Cited by 59 publications

(29 citation statements)

References 37 publications

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“…In view of its novel structural features, the alternatively spliced receptor 39 kDa FSH-R3 identified in the developing ovary (12) that is distinctly different from R1 becomes a good candidate. Like other growth factor type I receptors, the FSH-R3 has a single transmembrane domain and undergoes dimerization in response to the action of FSH (10). We have also shown that this R3 type receptor but not the Gs coupled R1 receptor mobilizes Ca 2+ influx into the cell through L-Voltage Ca 2+ channels (21).…”

Section: Introductionmentioning

confidence: 76%

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Activation of Extracellular-regulated Kinase Pathways in Ovarian Granulosa Cells by the Novel Growth Factor Type 1 Follicle-stimulating Hormone Receptor

Babu¹,

Krishnamurthy²,

Chedrese³

et al. 2000

Journal of Biological Chemistry

104

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Section: Introductionmentioning

confidence: 76%

“…Specific binding was determined in presence of 1 µg of unlabeled oFSH. The number of receptor molecules present in either JC-R3 or JC-R1 cells was determined as described earlier (10).…”

mentioning

confidence: 99%

Activation of Extracellular-regulated Kinase Pathways in Ovarian Granulosa Cells by the Novel Growth Factor Type 1 Follicle-stimulating Hormone Receptor

Babu¹,

Krishnamurthy²,

Chedrese³

et al. 2000

Journal of Biological Chemistry

104

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“…However, recent studies have shown that action of FSH on its receptor also activates the Ca 2þ pathway [5]. Studies using Sertoli cells and granulosa cells have revealed the existence of alternatively spliced variants of Fshr having varied roles [6,7].…”

Section: Introductionmentioning

confidence: 99%

Demonstration of Follicle-Stimulating Hormone Receptor in Cauda Epididymis of Rat1

Dahia

Rao

2006

Biology of Reproduction

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FSH receptor has been shown to be specifically expressed only in the Sertoli cells in males. In one of our studies that consisted of deprival of endogenous FSH in immature rats and adult bonnet monkeys, atrophy of the epididymis was observed, cauda region being the most affected. Although epididymis is an androgen-dependent tissue, the changes in histology of the cauda region were observed without any associated change in the levels of testosterone in FSH-deprived animals. Considering this, it was of interest to evaluate the possibility of epididymis being a direct target for FSH action. In the present study, we have examined the expression of FSH receptor in the epididymis of rat and monkey. In the cauda region of rat epididymis, FSH receptor expression was demonstrated by RT-PCR and Northern and Western blot analyses. FSH receptor was found to be functional as observed by its ability to bind 125IoFSH, by an increase in cAMP production, and by BrdU incorporation following addition of FSH under in vitro conditions. These results suggest the possibility of a role for FSH in regulating the growth of the epididymis.

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“…The FSH-R, derived from a single gene, is produced as a Gs-protein coupled, seven-transmembrane receptor, which activates several signaling pathways to integrate target cellular activities [1][2][3]. In the testis, FSH has a differential effect on Sertoli cells in accordance with the different stages of the cycle of the seminiferous epithelium.…”

Section: Introductionmentioning

confidence: 99%

Structural and Functional Modifications of Sertoli Cells in the Testis of Adult Follicle-Stimulating Hormone Receptor Knockout Mice1

Grover

Sairam

Smith

et al. 2004

Biology of Reproduction

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Follicle stimulating hormone (FSH) plays important roles during testicular development and in the maintenance of spermatogenesis in the adult. However, the cellular events or pathways that FSH regulates to achieve these effects in Sertoli cells, where the FSH receptors (FSH-R) are located, is still not fully elucidated. The development of FSH-R knockout (FORKO) mice provides a model to examine alterations in testicular structure and function in its absence. To this end, light (LM) and electron microscopic (EM) analyses of perfusion-fixed testes of wild-type and FORKO mice of different ages were performed. Under the LM, a significant reduction was noted in the profile area of seminiferous tubules of FORKO mice compared with their wild-type counterparts at different ages. In addition, FORKO testes revealed large irregularly shaped spaces within the seminiferous epithelium, extending from the base to the lumen. Such spaces were often separated by anastomotic cords of spherical germ cells or completely surrounded elongating spermatids. This phenotype was restricted to half or less of the circumference of only some tubules, but was seen at all stages. EM analyses revealed that the spaces corresponded to an apparent accumulation of fluid in the Sertoli cell cytoplasm, coincident with an absence of the fine flocculent ground substance seen in wild-type mice. However, the Sertoli organelles, while less prominent, appeared intact and to be floating in the enlarged fluid-filled cytoplasm. Functionally, androgen-binding protein (ABP), a major secretory protein of Sertoli cells, was dramatically reduced in FORKO mice. These results suggest that FSH-R signaling normally maintains water balance in Sertoli cells in addition to regulating ABP production.

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Alternative splicing converts the G-protein coupled follitropin receptor gene into a growth factor type I receptor: Implications for pleiotropic actions of the hormone

Cited by 59 publications

References 37 publications

Activation of Extracellular-regulated Kinase Pathways in Ovarian Granulosa Cells by the Novel Growth Factor Type 1 Follicle-stimulating Hormone Receptor

Activation of Extracellular-regulated Kinase Pathways in Ovarian Granulosa Cells by the Novel Growth Factor Type 1 Follicle-stimulating Hormone Receptor

Demonstration of Follicle-Stimulating Hormone Receptor in Cauda Epididymis of Rat1

Structural and Functional Modifications of Sertoli Cells in the Testis of Adult Follicle-Stimulating Hormone Receptor Knockout Mice1

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