Alternative splicing of the BSC1 gene generates tissue-specific isoforms in the German cockroach

Liu, Zhiqi; Chung, Inbum; Dong, Ke

doi:10.1016/s0965-1748(00)00178-8

Cited by 30 publications

(45 citation statements)

References 42 publications

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“…Like the VGSC a-subunit genes, the D. melanogaster sodium channel 1 (DSC1) gene (Salkoff et al, 1987) and the orthologous Blatella germanica BSC1 gene (Liu et al, 2001) belong to the 4 ¥ 6 TM domain family. Although originally classified as sodium channels based on their similarity to para, they actually encode calcium-selective channels (Zhou et al, 2004).…”

Section: Sodium Channel 60ementioning

confidence: 99%

Identification of ion channel genes in the Acyrthosiphon pisum genome

Dale

Jones

Tamborindeguy

et al. 2010

Insect Molecular Biology

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Abstracti mb_975 141..154Aphids are major pests of crops, causing hundreds of millions of dollars worth of damage annually. Ion channel proteins are often the targets of modern insecticides and mutations in ion channel genes can lead to resistance to many leading classes of insecticides. The sequencing of the pea aphid, Acyrthosiphon pisum, genome has now allowed detailed in silico analysis of the aphid ion channels. The study has revealed significant differences in the composition of the ion channel families between the aphid and other insects. For example A. pisum does not appear to contain a homologue of the nACh receptor alpha 5 gene whilst the calcium channel beta subunit has been duplicated. These variations could result in differences in function or sensitivity to insecticides. The genome sequence will allow the study of aphid ion channels to be accelerated, leading to a better understanding of the function of these economically important channels. The potential for identifying novel insecticide targets within the aphid is now a step closer.

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Section: Sodium Channel 60ementioning

confidence: 99%

Identification of ion channel genes in the Acyrthosiphon pisum genome

Dale

Jones

Tamborindeguy

et al. 2010

Insect Molecular Biology

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“…Our previous RT-PCR analysis showed that the BgNa v transcript was not only expressed abundantly in the nerve cord and leg but also detectable in ovary and gut (likely in neurons that innervate these tissues) (30). To determine whether the F-to-S change occurs in the ovary, we isolated 553 cDNA clones by using RNA from the ovary.…”

Section: F1919s Is the Results Of A U-to-c Editing Eventmentioning

confidence: 99%

Persistent tetrodotoxin-sensitive sodium current resulting from U-to-C RNA editing of an insect sodium channel

Liu

Song

Dong

2004

Proc. Natl. Acad. Sci. U.S.A.

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The persistent tetrodotoxin (TTX)-sensitive sodium current, detected in neurons of many regions of mammalian brains, is associated with many essential neuronal activities, including boosting of excitatory synaptic inputs, acceleration of firing rates, and promotion of oscillatory neuronal activities. However, the origin and molecular basis of the persistent current have remained controversial for decades. Here, we provide direct evidence that U-to-C RNA editing of an insect sodium channel transcript generates a sodium channel with a persistent current. We detected a persistent TTX-sensitive current in a splice variant of the cockroach sodium channel gene BgNa v (formerly para CSMA ). Site-directed mutagenesis experiments revealed that an F-to-S change at the C-terminal domain of this variant was responsible for the persistent current. We demonstrated that this F-to-S change was the result of a U-to-C RNA editing event, which also occurred in the Drosophila para sodium channel transcript. Our work provides direct support for the hypothesis that posttranscriptional modification of a conventional transient sodium channel produces a persistent TTX-sensitive sodium channel.

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“…DSC1 plays a role in the olfactory system (Kulkarni et al, 2002) as it has been found in the peripheral nervous system and also at high density in the synaptic regions. DSC1 is sensitive to tetradotoxin, a specific Na V blocker (Zhang et al, 2011), while BSC1, its homologous in B. germanica, has also been identified as a putative sodium channel, being expressed in the cockroach nerve cord, muscle, gut, fat body and ovary (Liu et al, 2001). Neither DSC1 nor BSC1, however, mapped with any locus related to insecticide resistance Salkoff et al, 1987).…”

Section: Molecular Biology Of the Insect Na V And The Kdr Mutationmentioning

confidence: 98%

“…The aminoacid sequences translated from optional exons are conserved and generally consist of intracellular domains of the channel, suggesting functional relevance to these events. Na V transcript diversity derived from alternative splicing has been investigated in insects of many orders, revealing a high level of conservation, as shown in the cockroach B. germanica (Liu et al, 2001;Song et al, 2004), the silk worm Bombyx mori (Shao et al, 2009), the moth Plutella xylostella (Sonoda et al, 2008) and the mosquitoes An. gambiae (T. G. Davies et al, 2007a) and Ae.…”

Section: 1mentioning

confidence: 99%