Alternative splicing of two translesion synthesis DNA polymerases from Arabidopsis thaliana

Santiago, María Jesús; Alejandre-Durán, Encarna; Ruiz-Rubio, Manuel

doi:10.1016/j.plantsci.2009.01.018

Cited by 6 publications

(5 citation statements)

References 36 publications

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“…OsFKBP59 and OsFKBP44 have been previously predicted to be cytoplasmic and mitochondrial, respectively (Nigam et al 2008); however, our predictions indicate nuclear localisation for both. Characteristic 'KRAKR' and 'KKARK' motifs at extreme C-termini of OsFKBP59 and OsFKBP44 respectively, provide NLS for numerous eukaryotic nuclear proteins (Aster et al 1997;Imamura et al 2001;Santiago et al 2009) and may be translocation domains for these rice FKBPs.…”

Section: Nuclear Fkbpsmentioning

confidence: 99%

Genome-wide analysis of genes encoding FK506-binding proteins in rice

Gollan

Bhave

2009

Plant Mol Biol

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The FK506-binding proteins (FKBPs) are a class of peptidyl-prolyl cis/trans isomerase enzymes, some of which can also operate as molecular chaperones. FKBPs comprise a large ubiquitous family, found in virtually every part of the cell and involved in diverse processes from protein folding to stress response. Higher plant genomes typically encode about 20 FKBPs, half of these found in the chloroplast thylakoid lumen. Several FKBPs in plants are regulators of hormone signalling pathways, with important roles in seed germination, plant growth and stress response. Some FKBP isoforms exists as homologous duplicates operating in finely tuned mechanisms to cope with abiotic stress. In order to understand the roles of the plant FKBPs, especially in view of the warming environment, we have identified and analysed the gene families encoding these proteins in rice using computational approaches. The work has led to identification of all FKBPs from the rice genome, including novel high molecular weight forms. The rice FKBP family appears to have evolved by duplications of FKBP genes, which may be a strategy for increased stress tolerance.

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Section: Nuclear Fkbpsmentioning

confidence: 99%

Genome-wide analysis of genes encoding FK506-binding proteins in rice

Gollan

Bhave

2009

Plant Mol Biol

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“…For example, genes encoding At-RAD1/UHV1 (homologous to yeast RAD1 and human XPF DNA repair endonuclease) and AtPOLK polymerase generate AS isoforms in a tissue-specific pattern (Vonarx et al, 2002;Garcia-Ortiz et al, 2004;Garcia-Ortiz et al, 2007). Two Arabidopsis translesion synthesis DNA polymerases, AtREV and AtPOLH, are regulated by AS, and complementation analysis of AtPOLH AS isoforms in Rad30-deficient yeast showed that the AtPOLH C-terminus is required for functional activity (Santiago et al, 2009). Several studies also reveal differential AS in DNA repair genes in crop plants, such as rice class II DNA photolyase (Hirouchi et al, 2003), endonuclease OsMUS81 (Mimida et al, 2007), and checkpoint protein OsRad9 (Li et al, 2017).…”

Section: Alternative Splicing and Dna Damage Response Insights From mentioning

confidence: 99%

Alternative Splicing and DNA Damage Response in Plants

2020

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Plants are exposed to a variety of abiotic and biotic stresses that may result in DNA damage. Endogenous processes -such as DNA replication, DNA recombination, respiration, or photosynthesis -are also a threat to DNA integrity. It is therefore essential to understand the strategies plants have developed for DNA damage detection, signaling, and repair. Alternative splicing (AS) is a key post-transcriptional process with a role in regulation of gene expression. Recent studies demonstrate that the majority of intron-containing genes in plants are alternatively spliced, highlighting the importance of AS in plant development and stress response. Not only does AS ensure a versatile proteome and influence the abundance and availability of proteins greatly, it has also emerged as an important player in the DNA damage response (DDR) in animals. Despite extensive studies of DDR carried out in plants, its regulation at the level of AS has not been comprehensively addressed. Here, we provide some insights into the interplay between AS and DDR in plants.

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“…Pol is encoded by the Arabidopsis POLH gene. The POLH transcript is ubiquitously expressed and alternatively spliced, and AtPol exhibits similar levels of activity in vitro as the human protein (Hoffman et al, 2008;Santiago et al, 2008aSantiago et al, , 2009. AtPOLH overexpression results in increased UV tolerance, while AtPOLH loss of function results in weak UV sensitivity, but enhances the sensitivity of the rev3 mutant, evidence that Pol is acting independently of Pol (Curtis & Hays, 2007;Santiago et al, 2008b).…”

Section: Translesion Synthesismentioning

confidence: 99%