Alternative to antibiotics against Pseudomonas aeruginosa: Effects of Glycyrrhiza glabra on membrane permeability and inhibition of efflux activity and biofilm formation in Pseudomonas aeruginosa and its in vitro time-kill activity

Chakotiya, Ankita Singh; Tanwar, Ankit; Narula, Alka; Sharma, Rakesh Kumar

doi:10.1016/j.micpath.2016.07.001

Cited by 49 publications

(37 citation statements)

References 20 publications

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“…This trend in antibiotic resistant bacterial infections is of serious global concern, fueled by the lack of effective antibiotics available and few being developed. 3 GLY, derived from Glycyrrhiza glabra, is antimicrobial and anti-inflammatory 6 ; it has many additional effects 6 and has shown promise against non-MDR isolates 7,8,16,19,20 and our current work shows it is effective against MDR isolates. Clinically, GLY also has been successfully used to treat blepharitis 28 and hepatitis patients 29,30 with no toxicity.…”

Section: Discussionmentioning

confidence: 61%

“…are used to determine antimicrobial profiles. 16,20,33,34 Using broth microdilution, we found that GLY reduced growth in both MDR clinical isolates, one from sputum and one from a keratitis patient. The ocular isolate was more sensitive to GLY which was somewhat puzzling and may reflect differences in virulence factors (e.g., biofilm production, or number of efflux pumps) and requires follow up studies to determine the reason(s).…”

Section: Discussionmentioning

confidence: 93%

“…14,15 GLY reduces proinflammatory mediators and has direct antimicrobial effects, reducing plate count after corneal infection with a non-MDR clinical (keratitis) PA isolate, KEI 1025 by~4.5 logs. 7 In addition, GLY has been shown to enhance the bioavailability of drugs, including antibiotics, 16,17 making them more effective at lower concentrations. Lower bacterial membrane permeability, overexpression of efflux pumps, biofilm, and antibiotic inactivating enzymes are major contributors to intrinsic multidrug resistance in PA. 18 Thus, the objectives of this study are to test GLY (with/without ciprofloxacin), and its effects on several of these contributors to resistance in MDR PA isolates from systemic and ocular sources.…”

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confidence: 99%

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Glycyrrhizin Use for Multi-Drug Resistant Pseudomonas aeruginosa: In Vitro and In Vivo Studies

Hazlett

Ekanayaka

McClellan

et al. 2019

Invest. Ophthalmol. Vis. Sci.

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PURPOSE.Our purpose was to test glycyrrhizin (GLY) effects and ciprofloxacin interactions on multidrug resistant (MDR) isolates of Pseudomonas aeruginosa in vitro and in vivo in a mouse model of keratitis. METHODS.A Hardy-disk tested antibiotic sensitivity of isolates MDR9 (nonocular) and B1045 (ocular). GLY MIC (both isolates) and ciprofloxacin was determined spectrophotometrically. A live/dead assay using confocal microscopy and plate count, tested GLY effects on bacterial permeabilization/viability. Proteomics profiled bacterial efflux pumps (MDR9 vs. PAO1); RT-PCR comparatively tested GLY effects on their mRNA expression levels. The activity of efflux pumps was tested using ethidium bromide (EB); and scanning electron microscopy (SEM) visualized the effects of GLY treatment of bacteria. A combination of GLY and ciprofloxacin was tested in C57BL/6 mice (begun 18 hours after infection) and disease scored, photographed and MPO and plate counts done.RESULTS. MDR9 was resistant to 6/12 and B1045 to 7/12 antibiotics (both to ciprofloxacin). MIC GLY for MDR9 was 40 mg/mL and 15 mg/mL for B1045. Ciprofloxacin MIC (32 lg/ mL) was reduced 2-fold to 16 lg/mL when ciprofloxacin and GLY were combined. GLY altered bacterial membrane permeability and reduced viability. Proteomics revealed increased efflux pumps in MDR9 versus PAO1; GLY reduced their mRNA expression levels and EB suggested decreased activity. In C57BL/6 mice, treatment with GLY and ciprofloxacin versus ciprofloxacin, significantly reduced clinical scores, plate count, and MPO.CONCLUSIONS. GLY decreases MDR by: altering bacterial parameters, including viability and efflux pump activity. In vivo, it increases the effectiveness of ciprofloxacin, reducing ocular disease, plate count, and MPO activity.

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Section: Discussionmentioning

confidence: 61%

Section: Discussionmentioning

confidence: 93%

mentioning

confidence: 99%

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Glycyrrhizin Use for Multi-Drug Resistant Pseudomonas aeruginosa: In Vitro and In Vivo Studies

Hazlett

Ekanayaka

McClellan

et al. 2019

Invest. Ophthalmol. Vis. Sci.

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“…It provides broad prospects of applying of glycyrrhizic acid as a multifunctional DDS. It was also established that GA enhance absorption effects of the variety of drugs (Kong et al, 2017;Sapra et al, 2008;Radwant and Aboul-Enein, 2002;Chen et al, 2009;Chakotiya et al, 2016). In view of the fact that the movement of small drug molecules through gastrointestinal epithelium is regulated by the distension and constriction of the tight junctions, observed effects could be connected with the ability of glycyrrhizin to enhance the penetration of molecules into the cells.…”

Section: Permeability Enhancement and Membrane-modifying Activitymentioning

confidence: 99%

Glycyrrhizic acid as a multifunctional drug carrier – From physicochemical properties to biomedical applications: A modern insight on the ancient drug

Selyutina

Polyakov

2019

International Journal of Pharmaceutics

161

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A B S T R A C TGlycyrrhizic acid is the main active component of Licorice root which has been known in traditional Chinese and Japanese medicine since ancient times. In these cultures glycyrrhizic acid (GA) is one of the most frequently used drugs. However, only in 21-st century a novel unusual property of the GA to enhance the activity of other drugs has been discovered. The review describes briefly the experimental evidences of wide spectrum of own biological activities of glycyrrhizic acid as well as discusses the possible mechanisms of the ability of GA to enhance the activity of other drugs. We have shown that due to its amphiphilic nature GA is able to form self-associates in aqueous and non-aqueous media, as well as water soluble complexes with a wide range of lipophilic drugs. The main purpose of our review is to focus reader's attention on physicochemical studies of the molecular mechanisms of GA activity as a drug delivery system (DDS). In our opinion, the most intriguing feature of glycyrrhizic acid which might be the key factor in its therapeutic activity is the ability of GA to incorporate into the lipid bilayer and to increase the membrane fluidity and permeability. The ability of biomolecules and their aggregates to change the properties of cell membranes is of great significance, from both fundamental and practical points of view. monstrated the ability of GA to interact with cell membrane and to https://doi.

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“…A study indicated the antipseudomonal activity of G. glabra and one of its compounds (14). The inhibitory effect of G. glabra on Helicobacter pylori was studied in-vitro and indicated that it can have effects similar to those of metronidazole (15).…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Some Medicinal Plants Extracts for Potential Antifungal Activity

Abkhoo

Jahani

2016

Int J Infect

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Background: Peganum harmala, Glycyrrhiza glabra, Mentha spicata and Rosmarinus officinalis are often used in traditional medicine. Objectives: The current study aimed to screen the antifungal activity of ethanolic extracts of P. harmala, G. glabra, M. spicata and R. officinalis leaves collected from Sistan region, Iran against Fusarium oxysporum. Methods: Minimal inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of herbal extracts were determined. Results: Of the total herbal extracts, four showed fungistatic activities and two showed fungicidal activities. MIC values, ranged 6.25 -50 ppm and MFC values, ranged 12.5 -100 ppm. The ethanolic extract of G. glabra had the lowest MIC (6.25 ppm); while the ethanolic extract of R. officinalis had the highest MIC (50 ppm). Minimum bactericidal concentration (MBC) of the alcoholic extract of G. glabra showed the highest efficiency against F. oxysporum at the low value of 12.5 ppm. Conclusions:The results of the study showed the antifungal activity of medicinal plants against F. oxysporum. Especially, the bioactive compounds of G. glabra were effective to inhibit the growth of F. oxysporum.

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Alternative to antibiotics against Pseudomonas aeruginosa: Effects of Glycyrrhiza glabra on membrane permeability and inhibition of efflux activity and biofilm formation in Pseudomonas aeruginosa and its in vitro time-kill activity

Cited by 49 publications

References 20 publications

Glycyrrhizin Use for Multi-Drug Resistant Pseudomonas aeruginosa: In Vitro and In Vivo Studies

Glycyrrhizin Use for Multi-Drug Resistant Pseudomonas aeruginosa: In Vitro and In Vivo Studies

Glycyrrhizic acid as a multifunctional drug carrier – From physicochemical properties to biomedical applications: A modern insight on the ancient drug

Efficacy of Some Medicinal Plants Extracts for Potential Antifungal Activity

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