Alternative Transcription of Peroxisome Proliferator-Activated Receptor Gamma in the Liver Is Associated with Fatness of Chickens

Abstract

The expression of four transcription variant of peroxisome proliferatoractivated receptor gamma gene (PPARG) (XM_015292931.1; XM_015292932.1; XM_015292933.1 and NM_001001460.1) in the liver of broilers was measured and its correlation with abdominal fat weight and relative abdominal fat content was investigated. The study was conducted with 92 slow-growing crossbred chickens (Cobb males x indigenous Green-legged Partridge female chickens) divided into "fat" and "lean" groups, according to their abdominal fat yield. The NM_001001460.1 transcriptwas upregulated with ratio of means 4.26 (p≤0.01) in the "fat" group in relation to the "lean" group. Expression of this transcript was highly correlated with relative abdominal fat content (0.71, p≤0.01) and abdominal fat weight (0.59, p≤0.01). Two SNPs are located in putative transcription factor binding sites. Mutation-991C>A disrupts PPAR while mutation-884C>T disrupts C/EBP putative binding site. The gene expression analysis of PPARg showed that the expression of the transcripts (NM_001001460.1) was more than four times higher in fat than in lean chickens. These results point out that the peroxisome proliferator-activated receptor gamma NM_001001460.1 transcript could be candidate gene for determination of abdominal fat deposition in the chickens.

Hepatic Gene Expression Profiling of American Kestrels (Falco sparverius) Exposed In Ovo to Three Alternative Brominated Flame Retardants

“…Downregulation of PPAR-, impairs FAO and can increase lipid availability for adipose tissue accretion and protein synthesis, which are critical for maintaining hatchling growth [ 49 ]. PPAR-γ mainly targets fatty acid uptake and storage and regulates cholesterol homeostasis in the liver, the main lipogenic site in birds [ 50 ]. Upregulation of PPARG in chicken liver is correlated with body fat deposition [ 50 ], and, although the effects of hepatic downregulation in birds have not been fully explored, decreased expression of PPARG may prevent lipid accumulation in the liver [ 48 ].…”

“…PPAR-γ mainly targets fatty acid uptake and storage and regulates cholesterol homeostasis in the liver, the main lipogenic site in birds [ 50 ]. Upregulation of PPARG in chicken liver is correlated with body fat deposition [ 50 ], and, although the effects of hepatic downregulation in birds have not been fully explored, decreased expression of PPARG may prevent lipid accumulation in the liver [ 48 ]. Our results contrasted with those reported by Guo et al [ 51 ] in adult zebrafish; after 2 weeks of TBPH exposure, expression of PPAR genes increased.…”

Sex‐ and Developmental Stage–Related Differences in the Hepatic Transcriptome of Japanese Quail (Coturnix japonica) Exposed to 17β‐Trenbolone

“…Up‐regulation of PPARγ results in the reduction of intracellular and extracellular cholesterols in human hepatocytes (Han et al 2019) and increased adipogenesis in chickens and ducks (Hérault et al 2018; Parada et al 2018). As a key component of the steroidogenic pathway and a steroid precursor, cholesterol regulation could influence steroidogenesis.…”