Alternatively Activated M2 Macrophages Improve Autologous Fat Graft Survival in a Mouse Model through Induction of Angiogenesis

Phipps, Kyle D.; Gebremeskel, Simon; Gillis, Joshua A.; Hong, Paul; Johnston, Brent; Bezuhly, Michael

doi:10.1097/prs.0000000000000793

Cited by 73 publications

(67 citation statements)

References 29 publications

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“…This theory is further supported by the work of Eto et al, 42 who have shown that proliferating adipose-derived stem cells repopulate nonviable portions of the fat graft and are responsible for much of the ultimate graft volume. In contrast to our earlier work 25 and that of Yoshimura et al, 43 who suggest that infiltrating cell replacement and vascular ingrowth are key to final graft volume, we observed improvement in graft volume and quality without an increase in vascularity, suggesting that N-acetylcysteine does not act by stimulating angiogenesis. This is in keeping with the work of Kølle et al, who observed no increase in vascular density 4 months after implantation of grafts enriched ex vivo with adipose-derived stem cells.…”

Section: Discussioncontrasting

confidence: 84%

“…25,26 Baseline micro-computed tomographic scans were obtained at day 4 and then again at 1 and 3 months after injection using a Flex Triumph in vivo microcomputed tomographic scanner (Gamma Medica, Inc., Salem, N.H.). The imaging protocol was 8.53 minutes, with a voxel resolution of 102 μm with 512 views and x-ray voltage of 70 kVp with anode current of 160 mA.…”

Section: Volumetric Analysismentioning

confidence: 99%

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Effect of N-Acetylcysteine on Adipose-Derived Stem Cell and Autologous Fat Graft Survival in a Mouse Model

Gillis

Gebremeskel

Phipps

et al. 2015

Plastic and Reconstructive Surgery

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Section: Discussioncontrasting

confidence: 84%

Section: Volumetric Analysismentioning

confidence: 99%

Effect of N-Acetylcysteine on Adipose-Derived Stem Cell and Autologous Fat Graft Survival in a Mouse Model

Gillis

Gebremeskel

Phipps

et al. 2015

Plastic and Reconstructive Surgery

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“…[1][2][3][4][5]8,9,15,22 Autologous fat grafts were combined with numerous different agents in recent years to increase their viability and decrease the rate of resorption including stem cell therapies; growth factors such as platelet-derived growth factor (PDGF), basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), and epidermal growth factor (EGF); and other agents such as platelet-rich plasma (PRP), activated M2 macrophages, polymer therapy, and matrix metalloproteinase-2. [23][24][25][26][27][28][29][30] All of these agents and modalities demonstrated variable effects on the survival of fat grafts; however, none of them can be considered as the preferred method or agent for this issue. DFO is an ironchelating agent with numerous functions which directed us to research its effects on fat tissue transfer.…”

Section: Figmentioning

confidence: 99%

Effects of Deferoxamine on Fat Graft Survival

et al. 2016

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The most important problem in fat transplantation is the unpredictable rates of resorption. Deferoxamine (DFO) is an iron-chelating agent with many useful functions including stimulating angiogenesis and antioxidant nature. The purpose of the study is to evaluate the effects of DFO on fat graft viability in rat model. A total of 24 Wistar rats were divided into three groups and 0.5 g of the left inguinal fat pad was extracted. In control group, fat grafts were implanted to the parascapular area without performing any procedure. In sham group, they were implanted in 0.2 mL saline solution followed by serial saline injections for 1 month. In the study group, fat grafts were implanted in 0.2 mL saline solution and 300 mg DFO followed by serial DFO injections for 1 month. At the postoperative second month, fat grafts were taken back and sent for histopathologic examination. The weight measurements of biopsy specimens in the study group demonstrated significantly higher than in the other two groups. Inflammation and fibrosis rates were also found to be significantly higher in the study group compared with the other groups; however, no significant difference in the apoptosis rates was detected between the groups. Fat grafts enriched with DFO showed significant increase in fatty tissue content in the study group compared with the control and sham groups. DFO increases the fat graft survival in rats and it may be a useful addition in autologous fat grafting procedures to increase fat graft viability and obtain maximal long-term durability.

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“…To understand the effect of collagenase digestion and SVF supplementation on micro-grafts, we used x-ray microtomography (micro-CT) to serially and noninvasively track the volume retention of human adipose tissue in a modified, published xenograft model 141,142 . This afforded the ability to serially track the volume of the grafted fat without the need to sacrifice the animal at each time point, something that has not been performed extensively in the literature.…”

Section: Overview Of Thesismentioning

confidence: 99%

“…In that same vein, supplementing the grafts with anti-inflammatory cytokines to combat this inflammatory environment would be an interesting study to conduct in the future. Interestingly, Phipps et al supplemented fat grafts with M2 macrophages and reported an increase in fat graft retention in a murine allograft model 142 . A better understanding of the effect of collagenase digestion on the proteins and cytokines within the adipose tissue may inform scientists and clinicians of ways to combat the decreased volume retention.…”

Section: Mechanism Responsible For Collagenase-induced Decreased Viabmentioning

confidence: 99%

Examination of Microvascular Remodeling to Enhance Autologous Fat Grafting

Seaman¹

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in the UVa Department of Chemistry for allowing me to be a part of the development of an exciting clinical diagnostic device. To the past and present Peirce Pirates/members of the lab, it has been a pleasure working with all of you. I'll miss experiencing the joys (and difficulties) of research, our coffee runs, Last Thursday happy hours, croquet matches, our shared commiserating, and bocce tournaments, but I look forward to our continued friendships. To Bryan, Jason, and Tom, thank you for showing me the ropes and getting me started when I was a new graduate student. Alongside me throughout my graduate work-Joe, Kyle, and Anthony-thank you for being great friends, coworkers, and scientists to help refine my work. Anthony, you deserve a special thank you for keeping the lab running smoothly even when we tried to veer off course. To Molly, Bruce, and Clif, I appreciate the help that you all have provided and hope that I have reciprocated in some way to your work and look forward to seeing all of your successes in the future. To the undergraduates that worked directly with me-Yiqi, Ethan, and Cat-thank you for helping with experiments, analyzing data, and for teaching me about the UVa culture. I'm very fortunate to work with all of these talented individuals and am excited for our future friendships. Thank you to all of my fellow graduate students, their significant others, and my other Charlottesville friends that have been a part of many fun and exciting adventures along the way. I've forged a new set of friends here in Charlottesville (way too long to list) and I am looking forward to our continued relationships. I would especially like to thank Eric, Sameer, and Edik for their continued friendship and encouragement. Eric, thanks for teaching me everything there is to know about beer and for explaining the game of rugby to me (maybe one day I'll understand it). Sameer, thank you for always being willing to talk about sports and, in general, arguing (discussing?) Georgia Tech and UVa sports. Edik, thanks for being so positive about everything and for your happy-go-lucky/West coast attitude that brought a fresh perspective to my life. I am truly lucky to develop and maintain so many great relationships during graduate school. My family deserves much of the credit for my successes. Mom and Dad, thank you for always pushing me to try my hardest and for enabling me to journey down v this privileged path. While my parents don't know exactly what I do, they have never stopped telling me how proud they are of me and have always been my number one supporter. To answer your recurring question Mom and Dad, "school is going well". I love you, Mom and Dad. To my brother/role model/best friend Marc, thank you for setting such a great standard for me to live up to. I've followed your example from when we were young and will continue to look up to you. To Amanda, I also want to thank you for your support over the years. I also owe gratitude to extended family members that have supported me along the way-Aunt Nancy, AK and UA, t...

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Alternatively Activated M2 Macrophages Improve Autologous Fat Graft Survival in a Mouse Model through Induction of Angiogenesis

Abstract: M2 macrophages improve autologous fat graft volume retention by stimulating angiogenesis. These findings provide proof-of-principle for development of fat grafting techniques that harness reparative properties of M2 macrophages.

Cited by 73 publications

References 29 publications

Effect of N-Acetylcysteine on Adipose-Derived Stem Cell and Autologous Fat Graft Survival in a Mouse Model

Effect of N-Acetylcysteine on Adipose-Derived Stem Cell and Autologous Fat Graft Survival in a Mouse Model

Effects of Deferoxamine on Fat Graft Survival

Examination of Microvascular Remodeling to Enhance Autologous Fat Grafting

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