Alternatively Spliced BnaPAP2.A7 Isoforms Play Opposing Roles in Anthocyanin Biosynthesis of Brassica napus L.

Chen, Daozong; Liu, Yi; Yin, Shuai; Qiu, Jie; Jin, Qingdong; King, Graham J.; Wang, Jing; Ge, Xianhong; Li, Zaiyun

doi:10.3389/fpls.2020.00983

Cited by 38 publications

(32 citation statements)

References 70 publications

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“…What clearly appears is an important role of these post-transcriptional gene regulatory mechanisms in the modulation of flavonoid metabolism and anthocyanin biosynthesis. This has been recently described in several species, including tea plant (Camellia sinensis) [78], Brassica napus [79], and chrysanthemum (Chrysanthemum morifolium) [80], where either MYB or bHLH regulatory genes were found to undergo AS to fine-tune anthocyanin production in specific tissues.…”

Section: Splicing Mutations and Alternative Transcripts Can Affect An...mentioning

confidence: 90%

Fruit Colour and Novel Mechanisms of Genetic Regulation of Pigment Production in Tomato Fruits

Gonzali

Perata

2021

Horticulturae

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Fruit colour represents a genetic trait with ecological and nutritional value. Plants mainly use colour to attract animals and favour seed dispersion. Thus, in many species, fruit colour coevolved with frugivories and their preferences. Environmental factors, however, represented other adaptive forces and further diversification was driven by domestication. All these factors cooperated in the evolution of tomato fruit, one of the most important in human nutrition. Tomato phylogenetic history showed two main steps in colour evolution: the change from green-chlorophyll to red-carotenoid pericarp, and the loss of the anthocyanic pigmentation. These events likely occurred with the onset of domestication. Then spontaneous mutations repeatedly occurred in carotenoid and phenylpropanoid pathways, leading to colour variants which often were propagated. Introgression breeding further enriched the panel of pigmentation patterns. In recent decades, the genetic determinants underneath tomato colours were identified. Novel evidence indicates that key regulatory and biosynthetic genes undergo mechanisms of gene expression regulation that are much more complex than what was imagined before: post-transcriptional mechanisms, with RNA splicing among the most common, indeed play crucial roles to fine-tune the expression of this trait in fruits and offer new substrate for the rise of genetic variables, thus providing further evolutionary flexibility to the character.

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Section: Splicing Mutations and Alternative Transcripts Can Affect An...mentioning

confidence: 90%

Fruit Colour and Novel Mechanisms of Genetic Regulation of Pigment Production in Tomato Fruits

Gonzali

Perata

2021

Horticulturae

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“…The variegated testa is composed of the colored area and non-colored area, in which the colored area also includes purple, red, pink, and others. Different types and contents of anthocyanins will cause peanut testa to show different colors.Anthocyanins are natural antioxidants 3 , which have a strong a nity for collagen 4 , and have many pharmacological functions in anti-oxidation, anti-in ammatory, eyesight protection, antibacterial, and other aspects 5 .…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic and Metabolomic Joint Analysis Reveals Distinct Flavonoid Biosynthesis Regulation for Variegated Testa Color Development in Peanut (Arachis Hypogaea L.)

Hou

et al. 2020

Preprint

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Peanut (Arachis hypogaea L.) is one of the important oil and economic crops, among which the variegated testa peanut is a unique member. But the molecular mechanisms underlying the pigment synthesis in variegated testa are still unclear. Differentially expressed genes (DEGs) in pigment metabolism pathway in colored area indicated there were 27 DEGs highly related to the synthesis of variegated testa color among 1,050 DEGs,which were 13 up-regulated and 14 down-regulated,consisting of 3 PALs, 1 C4H, 2 CHSs, 1 F3H, 1 F3'H, 2 DFRs, 2 LARs, 2 IAAs, 4 bHLHs and 9 MYBs. GO analysis indicated DEGs were similarly enriched in 3 branches.KEGG analysis suggested flavonoid biosynthesis is the most direct metabolic pathway for the synthesis of testa variegation. The liquid chromatography tandem mass spectrometry (LC-MS/MS) results showed that cyanidin and delphinidin were the main metabolites that caused the color difference between the colored area and the non-colored area. Through the verification of 20 DEGs via qPCR, the results were consistent with that of transcriptome sequencing in 4 comparison groups. The results in this study lay the foundation for revealing the molecular regulation mechanisms of anthocyanin synthesis in variegated testa peanut.

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“…The read counts of each gene were calculated using the htseq-count function in htseq software (Anders et al, 2015). The R package DEseq2 (v1.16.1) was used to identify the differentially expressed genes (DEGs) between leaves of different colors based on the following criteria: padj < 0.01 & log2FoldChange > 2 (Chen et al, 2020b).…”

Section: Expression Pro Les Analysis Based On Rna-seq Datamentioning

confidence: 99%

Genome-wide identification of R2R3-MYB gene family and association with anthocyanin biosynthesis in Brassica species

Chen

Dai

et al. 2022

Preprint

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Brassica species include important oil crops and vegetables in the world. The R2R3-MYB gene participates in a variety of plant functions, including the activation or inhibition of anthocyanin biosynthesis. Although previous studies have reported its phylogenetic relationships, gene structures, and expression patterns in Arabidopsis, the number and sequence variation of this gene family in Brassica crops and its involvement in the natural quantitative variation in anthocyanin biosynthesis regulation are still largely unknown. In this study, by using whole genome sequences and comprehensive genome-wide comparative analysis among the six cultivated Brassica species, 1990 R2R3-MYB genes were identified in six Brassica species, respectively. These R2R3-MYB genes were phylogenetically clustered into 12 groups. The R2R3-MYB family between A and C subgenomes showed better collinearity than between B and C and between A and B. From comparing transcriptional changes of five Brassica species with the purple and green leaves for the detection of the R2R3-MYB genes associated with anthocyanin biosynthesis, 7 R2R3-MYB genes were co-differentially expressed. The promoter and structure analysis of these genes showed that some variations between non-coding region, but they were highly conserved at the protein level and spatial structure. Co-expression analysis of anthocyanin-related genes and R2R3-MYBs indicated that MYB90 was strongly co-expressed with TT8, and they were co-expressed with structural genes F3H, LDOX, ANS and UF3GT at the same time. These results further clarified the roles of the R2R3-MYBs for leaf coloration in Brasica species, which provided new insights into the functions of the R2R3-MYB gene family in Brasica species.

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Alternatively Spliced BnaPAP2.A7 Isoforms Play Opposing Roles in Anthocyanin Biosynthesis of Brassica napus L.

Cited by 38 publications

References 70 publications

Fruit Colour and Novel Mechanisms of Genetic Regulation of Pigment Production in Tomato Fruits

Fruit Colour and Novel Mechanisms of Genetic Regulation of Pigment Production in Tomato Fruits

Transcriptomic and Metabolomic Joint Analysis Reveals Distinct Flavonoid Biosynthesis Regulation for Variegated Testa Color Development in Peanut (Arachis Hypogaea L.)

Genome-wide identification of R2R3-MYB gene family and association with anthocyanin biosynthesis in Brassica species

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