Altrenogest affects expression of galectin-3 and fibroblast growth factor 9 in the reproductive tract of sows

Baek, Sung-June; Sa, Soo-Jin; Jeong, Young-Dae; Cho, Eun-Seuk; Hong, Jian‐Hua; KIm, Young-Shin; Cho, Kyu-Ho; Park, Seol-Hwa; Kim, Kyung-Woon; Lee, Hwi-Cheul; Hochi, Shinichi; Lee, Seung Hwan; Choi, Inchul; Chung, Hak-Jae

doi:10.1080/10495398.2020.1726362

Cited by 4 publications

(2 citation statements)

References 19 publications

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“…ten boar/growth stage) at the National Institute of Animal Science and Swine Science Division using standard castration protocols [9].…”

Section: Methodsmentioning

confidence: 99%

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Association of the ubiquitin specific peptidase 9X -linked and Afadin expression patterns with sexual maturation in boar testis

Baek¹,

Lee²,

Kim³

et al. 2021

J Anim Sci Technol

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“…ten boar/growth stage) at the National Institute of Animal Science and Swine Science Division using standard castration protocols [9].…”

Section: Methodsmentioning

confidence: 99%

“…The National Institute of Animal Science Animal Care and Use Committee approved all animal procedures (No.2019-397). Testis tissue samples were obtained from 1, 6, and 12-month-old boar (Duroc; ten boar/growth stage) at the National Institute of Animal Science and Swine Science Division using standard castration protocols [ 9 ].…”

Section: Methodsmentioning

confidence: 99%

Association of the ubiquitin specific peptidase 9X -linked and Afadin expression patterns with sexual maturation in boar testis

Baek¹,

Lee²,

Kim³

et al. 2021

J Anim Sci Technol

Self Cite

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Detection of sow pregnancy in day-20 urine samples using monoclonal antibody against synthesized porcine early pregnancy factor: Preliminary results

Park,

Cho,

Kim

et al. 2024

Theriogenology

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In Vivo-Matured Oocyte Resists Post-Ovulatory Aging through the Hub Genes DDX18 and DNAJC7 in Pigs

Zhan,

Zhou,

Sun

et al. 2024

Antioxidants

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Assisted reproduction technology (ART) procedures are often impacted by post-ovulatory aging (POA), which can lead to reduced fertilization rates and impaired embryo development. This study used RNA sequencing analysis and experimental validation to study the similarities and differences between in vivo- and vitro-matured porcine oocytes before and after POA. Differentially expressed genes (DEGs) between fresh in vivo-matured oocyte (F_vivo) and aged in vivo-matured oocyte (A_vivo) and DEGs between fresh in vitro-matured oocyte (F_vitro) and aged in vitro-matured oocyte (A_vitro) were intersected to explore the co-effects of POA. It was found that “organelles”, especially “mitochondria”, were significantly enriched Gene Ontology (GO) terms. The expression of genes related to the “electron transport chain” and “cell redox homeostasis” pathways related to mitochondrial function significantly showed low expression patterns in both A_vivo and A_vitro groups. Weighted correlation network analysis was carried out to explore gene expression modules specific to A_vivo. Trait–module association analysis showed that the red modules were most associated with in vivo aging. There are 959 genes in the red module, mainly enriched in “RNA binding”, “mRNA metabolic process”, etc., as well as in GO terms, and “spliceosome” and “nucleotide excision repair” pathways. DNAJC7, IK, and DDX18 were at the hub of the gene regulatory network. Subsequently, the functions of DDX18 and DNAJC7 were verified by knocking down their expression at the germinal vesicle (GV) and Metaphase II (MII) stages, respectively. Knockdown at the GV stage caused cell cycle disorders and increase the rate of abnormal spindle. Knockdown at the MII stage resulted in the inefficiency of the antioxidant melatonin, increasing the level of intracellular oxidative stress, and in mitochondrial dysfunction. In summary, POA affects the organelle function of oocytes. A_vivo oocytes have some unique gene expression patterns. These genes may be potential anti-aging targets. This study provides a better understanding of the detailed mechanism of POA and potential strategies for improving the success rates of assisted reproductive technologies in pigs and other mammalian species.

show abstract

Altrenogest affects expression of galectin-3 and fibroblast growth factor 9 in the reproductive tract of sows

Cited by 4 publications

References 19 publications

Association of the ubiquitin specific peptidase 9X -linked and Afadin expression patterns with sexual maturation in boar testis

Association of the ubiquitin specific peptidase 9X -linked and Afadin expression patterns with sexual maturation in boar testis

Detection of sow pregnancy in day-20 urine samples using monoclonal antibody against synthesized porcine early pregnancy factor: Preliminary results

In Vivo-Matured Oocyte Resists Post-Ovulatory Aging through the Hub Genes DDX18 and DNAJC7 in Pigs

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