Aluminium Chloride Enhances Colchicine Production in Root Cultures of Gloriosa superba

Ghosh, Saswata; Ghosh, Biswajit; Jha, Sumita

doi:10.1007/s10529-006-0004-9

Cited by 49 publications

(23 citation statements)

References 25 publications

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“…However, colchicine content was found to be more in G. orangea than that of G. rothschildiana (Table 3). There are reports of the use of precursor molecules as well as elicitors for enhanced colchicine synthesis in regenerated plants of Gloriosa superba (Ghosh et al 2002, Ghosh et al 2006) and other species (Mukhopadhyay and Mukhopadhyay 2008a). Shivkumar and his associates (2004) had the opinion that the reduced level of precursors in callus culture of G. superba was responsible for reduced level of colchicine accumulation.…”

Section: Resultsmentioning

confidence: 99%

In vitro Microtuberization and Enhanced Colchicine Accumulation in Two Species of Gloriosa

2008

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SummaryThe present article describes an efficient technique for micropropagation and microtuberization of two species of Gloriosa, G. orangea and G. rothschildiana of Colchicaceae, the colchicine yielding plants. The plants were propagated in culture through apical shoot bud multiplication using modified Murashige and Skoog's nutrient medium (MS) supplemented with 6-benzylaminopurine (BAP) and a-naphthaleneacetic acid (NAA) in presence of two different concentrations of sucrose (3 and 6%). The process of microtuberization was found to be quite efficient in MS containing 3% sucrose, producing about 180-240 tubers per culture vessel within 6 months or less. On the other hand, the medium with 6% sucrose level induced microtuberization 2 weeks earlier with reduced rate of regeneration. The microtubers from 3% media showed 97% germination rate. The best result was obtained in a medium containing BAP (17.76 mM) and NAA (2.15 mM) with about 18-24 shoots and 14-21 microtubers/shoot. A 1.7 fold increase in colchicine accumulation in G. rothschildiana and a 2.0 fold increase in G. orangea estimated through High Pressure Liquid Chromatography (HPLC) were observed as compared to in vivo tubers. The extracted colchicine showed better efficacy than standard colchicine from Sigma Chemical Co. in treated root tip cells of Allium sativum L. and Mucuna pruriens L. In both these test plant systems, the extracted colchicine induced both polyploidy and diplochromatid formation at variable rates and the values were little higher in G. orangea than in G. rothschildiana. Moreover, G. orangea responded better than G. rothschildiana against the same concentrations of plant growth regulators and showed better colchicine accumulation as well. Key wordsThe species of Gloriosa of Colchicaceae (formerly under Liliaceae), distributed throughout tropical and sub-tropical regions of Asia and Africa, are medicinally important for treatment of chronic ulcers, hemorrhoids, leprosy and for inducing labor pains (Chopra et al. 1969). These plants contain the valuable alkaloids, the colchicine and colchicosides like another genus, Iphigenia under this family (Mukhopadhyay and Mukhopadhyay 2008a, b). The plants of Gloriosa are being overexploited for long time by the rural people for their medicinal purpose and that has caused its distribution very limited and so designated as threatened. Moreover, Gloriosa shows a very poor rate of vegetative propagation and one plant produces only one bi-forked tuber with one growing bud on top of each fork. The seed-grown plants, on the other hand, also take three to four years to flower and therefore, it is also not usually favored by the growers. In order to maintain different germplasms of these species of Gloriosa, suitable measures for rapid mass propagation and conservation are of much importance. Several

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Section: Resultsmentioning

confidence: 99%

In vitro Microtuberization and Enhanced Colchicine Accumulation in Two Species of Gloriosa

2008

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“…Root cultures of Gloriosa superba were initiated from 1 cm long root tip explants derived from in vitro regenerated plantlets on solid MS supplemented with 3 mg/l NAA and 1 mg/l BA (NB medium) at 24 ± 1ºC under complete darkness (Ghosh et al 2006(Ghosh et al , 2007. These cultures were maintained successfully in the same medium for six months with regular subculture after six weeks.…”

Section: Methodsmentioning

confidence: 99%

Role of Exogenous Carbohydrate and Amino Acid Sources on Biomass and Colchicine Production in Nontransformed Root Cultures of Gloriosa superba

Ghosh¹,

Ghosh²,

Jha

2016

Plant Tissue Cult. & Biotech.

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Elicitation strategies were studied for yield enhancement of colchicine, produced by root cultures of Gloriosa superba. Adventitious root cultures were established and grown in media containing 3 mg/l NAA and 1 mg/l BA. Root cultures showed variations in biomass as well as colchicine production in the presence of different exogenous carbohydrates and amino acid. Among the different sources of carbohydrates used -fructose, sucrose and dextrose gave a substantially higher biomass yield than the control. Maximum biomass was obtained in the presence of fructose. Root cultures growing in mannitol supplemented medium resulted in maximum accumulation of colchicine (0.32%). Among the amino acids, serine and phenylalanine significantly enhanced colchicine accumulation in root cultures. 0.02 mM glutamine supplemented media showed maximum (ten-fold) increase of root biomass.

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“…The enhancement of intracellular colchicine content was observed in the roots by 50-fold and 63-fold respectively. Ghosh et al (2006) reported that colchicine can also be applied in the lanolin paste or as a solution, for instance, on a cotton dot, placed in a leaf axil. Khan et al (2007) evaluated the enzyme inhibition activities of G. superba rhizomes extract against lipoxygenase, actylcholinesterase, butyrycholinesterase and urease in which wonderful inhibition was observed on lipoxygenease.…”

Section: B I O D I V E R S I T a Smentioning

confidence: 99%

Review: Current Advances in Gloriosa superba L.

Ade

Mahendra

2009

Biodiversitas

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Gloriosa superba L. is an important medicinal plant of Asia and Africa. It is used in diseases, like cancer, gout, scrofula and act as antipyretic, antihelmintic, purgative and antiabortive. It is a source of colchicines and colchicocides, which are very costly, being highly demanded by pharma industries. Due to excessive use of the plant for diverse medicinal purposes the species is on the verge of extinction and included in Red Data Book. The strenuous efforts of botanists, biotechnologists, policy makers and conservationists are required. It is a matter of great concern to conserve this plant otherwise we will be loosing it by 2020.The present review is focused on current status of the genus, source of alkaloids, poisonous nature, the strategies for its conservation and future perspectives of G. superba.

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Aluminium Chloride Enhances Colchicine Production in Root Cultures of Gloriosa superba

Cited by 49 publications

References 25 publications

In vitro Microtuberization and Enhanced Colchicine Accumulation in Two Species of Gloriosa

In vitro Microtuberization and Enhanced Colchicine Accumulation in Two Species of Gloriosa

Role of Exogenous Carbohydrate and Amino Acid Sources on Biomass and Colchicine Production in Nontransformed Root Cultures of Gloriosa superba

Review: Current Advances in Gloriosa superba L.

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