Aluminium polyphosphate complexes in the mycorrhizal basidiomycete Laccaria bicolor: A 27Al-nuclear magnetic resonance study

Martin, Francis; Rubini, P.; C�t�, Richard; Kottke, Ingrid

doi:10.1007/bf00196393

Cited by 16 publications

(20 citation statements)

References 21 publications

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“…The A1-F complex was later suggested to be involved in uptake and translocation of A1 (Nagata et al, 1993). A previous study showed that A1 was rapidly taken up and accumulated into polyphosphate complexes in the vacuoles of the mycorrhizal basidiomycete Laccaria bicolor (Martin et al, 1994). In the present study, signals corresponding to neither the A1-F complex nor to the catechins or polyphosphate complexes were observed (Fig.…”

Section: Discussioncontrasting

confidence: 49%

Internal Detoxification Mechanism of Al in Hydrangea (Identification of Al Form in the Leaves)

et al. 1997

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An internal detoxification mechanism for AI was investigated in an AI-accumulating plant, hydrangea (Hydrangea macrophylla), focusing on AI forms present in the cells. l h e leaves of hydrangea contained as much as 15.7 mmol AI kg-' fresh weight, and more than two-thirds of the AI was found in the cell sap. Using "AInuclear magnetic resonance, the dominant peak of AI was observed at a chemical shift of 11 to 12 parts per million in both intact leaves and the extracted cell sap, which is in good accordance with the chemical shift for the 1 :1 AI-citrate complex. Purification of cell sap by molecular sieve chromatography (Sephadex C-1 O) combined with ion-exclusion chromatography indicated that AI in fractions with the same retention time as citric acid contributed to the observed "AI peak,in the intact leaves. l h e molar ratio of AI to citric acid in the crude and purified cell sap approximated 1. The structure of the ligand chelated with AI was identified to be citric acid. Bioassay experiments showed that the purified AI complex from the cell sap did not inhibit root elongation of corn (Zea mays L.) and the viability of cells on the root tip surface was also not affected. lhese observations indicate that AI is bound to citric acid in the cells of hydrangea leaves.A1 toxicity is primarily characterized by the inhibition of root elongation, with no appearance of clearly identifiable symptoms in plant tops. This is because A13+, a toxic ionic species, has a high binding ability with cellular components of roots, and usually shows little translocation to the upper parts of plants. Most plants contain not more than 0.2 mg AI 8-l dry weight. However, some plants, known as "A1 accumulators," may contain more than 10 times this leve1 of A1 without any AI injury. Tea plants are typical AI accumulators; the A1 content in these plants can reach as high as 30 mg 8-l dry weight in old leaves, although

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Section: Discussioncontrasting

confidence: 49%

Internal Detoxification Mechanism of Al in Hydrangea (Identification of Al Form in the Leaves)

et al. 1997

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“…This suggests that polyphosphates in the spore have shorter chain lengths than those in the mycorrhizal state. We note that previous studies showing 31P spectra of ectomycorrhizal roots and free-living ectomycorrhizal (Martin et al, 1985b(Martin et al, , 1994MacFall et al, 1992;Gerlitz and Werk, 1994) contain small or no significant resonances from penultimate or terminal residues. The resonance in Figure 5 representing terminal residues (labeled "b) is larger than that from penultimate residues, which suggests either that there is additional contribution to this resonance either from another compound or from polyphosphates whose penultimate resonances are not visible under highresolution conditions.…”

Section: Cerminating Sporesmentioning

confidence: 51%

Partitioning of Intermediary Carbon Metabolism in Vesicular-Arbuscular Mycorrhizal Leek

et al. 1995

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“…However, such a complex has not been isolated or identified. In the vacuoles of the mycorrhizal basidiomycete Laccaria bicolor, Al was present in the form of polyphosphate complexes (Martin et al, 1994). Previously, we found that more than two-thirds of the Al in hydrangea leaves was present in the cell sap in a soluble form, and this Al form has been identified as Alcitrate complex at a 1:1 molar ratio of Al to citric acid (Ma et al, 1997a).…”

Section: Discussionmentioning

confidence: 98%

High Aluminum Resistance in Buckwheat1

1998

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Buckwheat (Fagopyrum esculentum Moench. cv Jianxi), which shows high Al resistance, accumulates Al in the leaves. The internal detoxification mechanism was studied by purifying and identifying Al complexes in the leaves and roots. About 90% of Al accumulated in the leaves was found in the cell sap, in which the dominant organic acid was oxalic acid. Purification of the Al complex in the cell sap of leaves by molecular-sieve chromatography resulted in a complex with a ratio of Al to oxalic acid of 1:3. A 13 C-nuclear magnetic resonance study of the purified cell sap revealed only one signal at a chemical shift 164.4 ppm, which was assigned to the Al-chelated carboxylic group of oxalic acid. A 27 Al-nuclear magnetic resonance analysis revealed one major signal at the chemical shift of 16.0 to 17.0 ppm, with a minor signal at the chemical shift of 11.0 to 12 ppm in both the intact roots and their cell sap, which is consistent with the Al-oxalate complexes at 1:3 and 1:2 ratios, respectively. The purified cell sap was not phytotoxic to root elongation in corn (Zea mays). All of these results indicate that Al tolerance in the roots and leaves of buckwheat is achieved by the formation of a nonphytotoxic Al-oxalate (1:3) complex.

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Aluminium polyphosphate complexes in the mycorrhizal basidiomycete Laccaria bicolor: A 27Al-nuclear magnetic resonance study

Cited by 16 publications

References 21 publications

Internal Detoxification Mechanism of Al in Hydrangea (Identification of Al Form in the Leaves)

Internal Detoxification Mechanism of Al in Hydrangea (Identification of Al Form in the Leaves)

Partitioning of Intermediary Carbon Metabolism in Vesicular-Arbuscular Mycorrhizal Leek

High Aluminum Resistance in Buckwheat1

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