Aluminum doped carbon nanodots as potent adjuvants on the mammalian macrophages

Ayaz, Furkan; Alaş, Melis Özge; Oğuz, Melike; Genç, Rükan

doi:10.1007/s11033-019-04701-1

Cited by 26 publications

(14 citation statements)

References 68 publications

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“…CNs were prepared using the method described in our previous report. 21 Primarily, 1.0 g of licorice root was extracted in ethanol/water (1:2 v/v) at 90°C in a beaker with stirring. Subsequently, this of 5 mL of licorice root extract and 2 mL of water/ethanol (1:1 v/v) were transferred into the Teflon oven vessel and kept at 250 °C for 30 mins.…”

Section: Materials and Methods Preparation And Characterization Of Cnsmentioning

confidence: 99%

“…The hydrodynamic size and surface zeta potential of the CNs were found to be 15-30 nm and 26.3mV±1.9 mV in dynamic light scattering (DLS) measurements, respectively. 21 The surface functional groups of test samples doped were examined by FTIR analysis, and it was observed that the presence of CN in these samples did not cause any change in functional groups even at high concentrations. Thus, it is understood that test samples protected its structure without chemical bonding with CN (Figure 2).…”

Section: Characteristics Of Denture Liners Incorporated With Cnsmentioning

confidence: 99%

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Karbon nanopartikül ilave edilmiş iki tip protez astarının renk değişimi, su emilimi ve suda çözünürlüğü

Ataol¹,

Ergün

Alaş

et al. 2022

Acta Odontologica Turcica

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OBJECTIVE:The aim of this manuscript was to investigate the effect of carbon nanoparticles (CNs) addition into denture liners on the color change and water absorption and solubility. MATERIALSAND METHOD: Test specimens (n=10) of Visco-Gel (Group VG) and Ufi-Gel (Group UG) were created either without (Groups C-as control and W) or with the incorporation of 0.5%, 1% and 10% CNs synthesized from licorice root plant. The size of the CNs were measured with dynamic light scattering. The color of each specimen was measured with a spectrophotometer. Water sorption and solubility were determined by weight measurement. The data were analyzed by two-way ANOVA for sample size estimation and Mann-Whitney U and Kruskal-Wallis tests for analyses of main outcomes with significance at the p<0.05 level.RESULTS: All CNs-added test groups showed statistically higher color change than control groups (p<0.0125). In Group UG, 10% CNs added test groups showed statistically higher water sorption and solubility values than Group C, W and 0.5% CNs added test groups (p<0.001). However, the addition of 0.5% and 1% CNs to Groups VG caused a statistical decrease in water sorption values compared to control groups and Group VG 10% (p<0.001).CONCLUSION: Color change of CNs added denture liners were not within clinically accepted range of color difference regardless of addition rate. CNs addition in tested denture liners promoted changes in the liners' sorption/ solubility. UG was more prone to increase in both water sorption and solubility than VG.

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Section: Materials and Methods Preparation And Characterization Of Cnsmentioning

confidence: 99%

Section: Characteristics Of Denture Liners Incorporated With Cnsmentioning

confidence: 99%

Karbon nanopartikül ilave edilmiş iki tip protez astarının renk değişimi, su emilimi ve suda çözünürlüğü

Ataol¹,

Ergün

Alaş

et al. 2022

Acta Odontologica Turcica

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“…Positive control wells had LPS only, and negative control wells did not have any chemicals. After 24 h of incubation, the media were checked for TNF and IL6 levels by BD ELISA kits by following BD's guidance protocols [16][17][18][19][20][21][22][23][24][25].…”

Section: Immunotoxicity Studiesmentioning

confidence: 99%

Injectable chitosan cryogel microspheres with biocompatible properties on mammalian macrophages in vitro

2021

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Macrophages play a vital role in rejection of the implant materials by producing pro-inflammatory TNF and IL6 cytokines. They are also important for the normal function of the immune system to fight against infections. Therefore, characterizing the immunotoxicity on this cell type will be informative for the medical use of the biocompatible materials. Chitosan-based materials have been widely considered as biocompatible and safe. They have been suggested for the bone implants. Studies suggest that depending on the setup and chitosan functionalization, the immune system may react differentially to the chitosanbased materials. In this study, we characterized the immunocompatibility of injectable chitosan cryogel microspheres on mammalian macrophages by measuring their effect on cell viability and these cells' ability to produce pro-inflammatory cytokines in the absence and presence of LPS, as a stimulant. Different sizes of microspheres were used to examine the effect of the size on their plausible effects. Our results suggest that independent of their size, chitosan cryogel microspheres were immunocompatible. They did not influence the cell viability and pro-inflammatory cytokine production by LPS-induced macrophages. Moreover, these materials lacked immunostimulatory activity as well, which means they would not activate the macrophages in the absence of any stimulant after their direct interaction with these cells. These properties are advantageous for their possible utilization in the medical fields as minimally invasive materials due to their injectable properties.

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“…In almost all of the cytotoxicity studies performed to date, CDs have been demonstrated to cause a negligible effect on cell viability at concentrations sufficient for drug delivery and bio-imaging. In the last 2 years, we have been focused on new types of CDs by tailoring the surface properties of them with the target of evaluating their potential in biological applications 16 , 44 , 45 . Very recently, utilizing different polymers as surface passivating agent and surface doping agent, we were able to show the immunostimulant and adjuvant-like effect of such CDs where no cytotoxic effect was observed 44 , 45 .…”

Section: Introductionmentioning

confidence: 99%

“…In the last 2 years, we have been focused on new types of CDs by tailoring the surface properties of them with the target of evaluating their potential in biological applications 16 , 44 , 45 . Very recently, utilizing different polymers as surface passivating agent and surface doping agent, we were able to show the immunostimulant and adjuvant-like effect of such CDs where no cytotoxic effect was observed 44 , 45 . CDs have been widely researched for nucleus-targeted delivery, nucleus labeling, photodynamic therapy, and optical monitoring of anticancer drugs 46 – 50 , however, so far there are few scientific reports in existence for addressing the genotoxic activities of CDs when they administrated on their own 51 – 56 .…”

Section: Introductionmentioning

confidence: 99%

DNA-damage and cell cycle arrest initiated anti-cancer potency of super tiny carbon dots on MCF7 cell line

Şimşek

Şüküroğlu

Yetkin

et al. 2020

Sci Rep

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While carbon-based materials have spearheaded numerous breakthroughs in biomedicine, they also have procreated many logical concerns on their overall toxicity. carbon dots (cDs) as a respectively new member have been extensively explored in nucleus directed delivery and bioimaging due to their intrinsic fluorescence properties coupled with their small size and surface properties. Although various in vitro/in vivo studies have shown that CDs are mostly biocompatible, sufficient information is lacking regarding genotoxicity of them and underlying mechanisms. This study aims to analyze the real-time cytotoxicity of super tiny CDs (2.05 ± 0.22 nm) on human breast cancer cells (MCF7) and human primary dermal fibroblast cell cultures (HDFa) by xCELLigence analysis system for further evaluating their genotoxicity and clastogenicity to evaluate the anti-tumor potential of cDs on breast adenocarcinoma. As combined with flow cytometry studies, comet assay and cytokinesisblock micronucleus assay suggest that the CDs can penetrate to the cell nuclei, interact with the genetic material, and explode DNA damage and G0/G1 phase arrest in cancer cells even at very low concentrations (0.025 ppm) which provide a strong foundation for the design of potentially promising cD-based functional nanomaterials for DnA-damage induced treatment in cancer therapy. Functional nanomaterials that can better target cancer cells that will improve prevention and therapy could be accomplished by the combined efforts of nanotoxicologists, cancer biologists and nanobiomaterial scientists focused on toxicology and related cancer therapy. Engineering carbon-based nanomaterials and their applications are among the most dynamic fields in modern advanced materials science and engineering 1-3. To date, various carbon nanomaterials such as carbon nanotubes 4,5 , fullerenes 6 , graphene 7,8 , graphene oxides 9 , carbon diamonds 10,11 , and carbon dots 12,13 have been synthesized and reported by various researchers. Among these, CDs have become particularly interesting because of their unique physical and chemical properties such as thermal and electrical conductivity, high mechanical strength together with their unique optic and fluorescence features 14-16. They have been used in bio-imaging, drug delivery, nucleus targeting, and labeling, photodynamic therapy, optoelectronics, solar cells, photocatalyst design, photodetectors, and many other biological and engineering fields 17-26. Several methods are available for the synthesis of CDs, namely, laser ablation 15 , electro-oxidation 27 or oxidative acid treatment 12 ; while nowadays, less laborsome and cheaper methods such as hydrothermal 28 and thermal synthesis 24,29,30 , microwave-assisted hydrothermal synthesis 31 , ultrasound synthesis 32 , etc. are popularly exercised. CDs can be synthesized either from inorganic materials 19,33 such as graphene 34 , carbon black 35 , and candle soot 12

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Aluminum doped carbon nanodots as potent adjuvants on the mammalian macrophages

Cited by 26 publications

References 68 publications

Karbon nanopartikül ilave edilmiş iki tip protez astarının renk değişimi, su emilimi ve suda çözünürlüğü

Karbon nanopartikül ilave edilmiş iki tip protez astarının renk değişimi, su emilimi ve suda çözünürlüğü

Injectable chitosan cryogel microspheres with biocompatible properties on mammalian macrophages in vitro

DNA-damage and cell cycle arrest initiated anti-cancer potency of super tiny carbon dots on MCF7 cell line

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