Alveolar Bone Osteoclast Profile in the Periodontitis Wistar Rats Model with the Snail Slime (Achatina Fulica) Application

Swastini, I Gusti Agung Ayu Putu; Mahadewa, Tjokorda Gde Bagus; Widyadharma, I Putu Eka

doi:10.3889/oamjms.2019.451

Cited by 6 publications

(3 citation statements)

References 11 publications

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“…Preparations were stained with one drop of mounting medium and covered with a glass deck. 18 Observation and counting of preparations under an Olympus BX41 light microscope (Tokyo,Japan) with 400x magnification with 3x field of view in 3 repeated calculations connected to a computer screen and counting the number of lymphocyte cells in three fields of view.…”

Section: Methodsmentioning

confidence: 99%

The effect of the pseudostem extract of ambon banana on the number of lymphocytes and fibroblasts in gingivitis : an experimental study

Andriani,

Medawati,

Lestra

et al. 2023

Padjadjaran J Dent

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Introduction: Gingivitis is an inflammation of the soft tissue that supports the teeth. Chemical treatment in the form of mouthwash, such as chlorhexidine, is often recommended. However, long-term use of chlorhexidine mouthwash will cause side effects, such as allergies, irritation of the oral cavity, and discoloration of the oral mucosa and teeth. Therefore, natural alternative materials containing flavonoids, saponins, and tannins that can cure gingivitis are needed. These ingredients are found in the pseudo stem of the Ambon banana. This research aims to analyze the effect of applying the pseudostem extract of Ambon banana on the number of lymphocytes and fibroblasts in treating gingivitis. Methods: This study was an in vivo laboratory experimental study with a post-test control group. The study used 45 Sprague Dawley rats which were divided into 3 groups, namely the positive control group (chlorhexidine gel), negative control (gel base), and treatment (Ambon Banana pseudostem extract gel). The gingiva on the mandibular incisor of the rat was ligated, then the gel was applied to the gingiva. Decapitation of rats was carried out on days 1, 3, 5, 7, and 9, and then made histological preparations with HE. Staining and observations were later made. Data analyzed using Kruskal-Wallis test. Results: The results showed that Ambon banana false stem extract gel reduced the number of lymphocyte cells with a significant difference of p=0.016 (p<0.05) and increased fibroblasts at p=0.85 (p>0.05). Conclusion: The pseudostem extract gel of Ambon banana reduced the number of lymphocyte cells and increased fibroblasts in gingivitis treatment.Keywords: fibroblasts, gingivitis, lymphocytes, pseudostem of ambon banana

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Section: Methodsmentioning

confidence: 99%

The effect of the pseudostem extract of ambon banana on the number of lymphocytes and fibroblasts in gingivitis : an experimental study

Andriani,

Medawati,

Lestra

et al. 2023

Padjadjaran J Dent

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“…Increased osteoclast activity accompanied by continuous extracellular matrix damage can trigger periodontal tissue damage which causes bone resorption (Maduratna and Setiawatie 2014). Proinflammatory cytokines, including TNF-α, IL-1, and IL-6, that trigger pro-resorptive actions are highly upregulated by A. actinomycetemcomitans, and thus promote osteoclast formation and bone resorption (Swastini et al 2019). A. actinomycetemcomitans infection leads to increases in the pro-inflammatory cytokines TNF-α, IL-17, and RANKL, and decreases in the anti-inflammatory cytokines IL-10, TGF-β, and OPG (Izawa et al 2014;Araujo-Pires et al 2015;Levy-Ontman et al 2017).…”

Section: Groupmentioning

confidence: 99%

Effectiveness of oral irrigation with an extract of green microalga Nannochloropsis oculata as an anti-inflammatory in rats infected with Aggregatibacter actinomycetemcomitans

et al. 2020

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Abstract. Revianti S, Andriani D, Parisihni K, Wahjuningsih E, Widyastuti. 2020. Effectiveness of oral irrigation with an extract of green microalga Nannochloropsis oculata as an anti-inflammatory in rats infected with Aggregatibacter actinomycetemcomitans. Biodiversitas 21: 2977-2981. Aggressive periodontitis is strongly correlated with Aggregatibacter actinomycetemcomitans, a periodontopathic bacterium, which releases endotoxins and lipopolysaccharides (LPS). LPS acts as a stimulus to a variety of host cells that can promote the expression of pro-inflammatory cytokines, such as the tumor necrosis factor-α (TNF-α), in periodontal disease. The marine green microalga Nannochloropsis oculata has anti-inflammatory properties. This study aimed to examine the anti-inflammatory efficacy of oral irrigation with N. oculata extract against periodontitis induced by A. actinomycetemcomitans in a rat model. Twenty-four male Wistar rats were divided randomly into four groups (n = 6). First control group was without any treatment, the second to fourth groups were infected with A. actinomycetemcomitans and then orally irrigated with N. oculata extract at concentrations of 2.375%, 2.5%, and 2.625%. The mandibles of the euthanized rats were hemisected to measure the expression of TNF-α, interleukin-10 (IL-10, an anti-inflammatory cytokine), and osteoprotegerin (OPG) by immunohistochemistry. The first group (control) showed significantly higher expression of TNF-α and significantly lower expression of IL-10 and OPG compared to the other groups. Oral irrigation with N. oculata extracts reduced the expression of TNF-α and enhanced the expression of IL-10 and OPG in rats infected with A. actinomycetemcomitans. The effective concentration of N. oculata extract as an anti-inflammatory for oral irrigation was 2.375%.

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“…10 Bone matrix and cells is formed by osteoblasts and resorbed by osteoclasts. 11 Osteoclasts are hematopoietic stem cells derived from the granulocyte-macrophage colony forming unit (CFU-GM), and osteoblasts are mesenchymal stem cells derived from Fibrocyte Colony Forming Unit (CFU-F). 12.13 Bone is composed of organic (collagen) and inorganic (hydroxyapatite and calcium phosphate) matrices.…”

Section: Introductionmentioning

confidence: 99%

<strong>The effect of brown algae (<em>Sargassum sp.</em>) gel on the number of osteoclasts in periodontitis rats</strong>

2022

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Introduction: Alveolar bone resorption in periodontitis is caused by increased activation of osteoclasts, causing an imbalance of bone remodeling. Periodontitis treatment can be done mechanically with Scaling and Root Planing and accompanied by metronidazole. Antibiotics have long-term drawbacks and can cause allergies, thus requiring an alternative to natural topical drugs such as brown algae gel. This study aimed to to analyzed the effect of brown algae gel (sargassum sp) on the number of osteoclasts of alveolar bone in periodontitis rats. Methods: The research method was a True Experimental with a pretest-posttest-only control group design. Rats were divided into three groups; negative control (K-) was not given any treatment, positive control (K+) was given metronidazole ties plus 25% concentration, and the treatment group (KP) was given brown algae gel (Sargassum sp) 75% concentration. Data were analyzed by Friedman and Kruskal Wallis test. Results:There was a significant difference in the number of osteoclasts on days 8, 10, and 12, the decrease in the number of osteoclasts was higher in the brown algae treatment group than the negative control group (p=0.051). In the positive control group, there was a significant difference in the decrease of the number of osteoclasts compared to the brown algae treatment group (p=0.029). The number of osteoclasts was significantly different on day 12 (p=0.026).Conclusion: Adhering brown algae (Sargassum sp.) gel decreases osteoclasts in periodontitis rats.Keywords: bone remodeling; bone resorption; brown algae gel (sargassum sp.); osteoclasts; periodontitis.

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Alveolar Bone Osteoclast Profile in the Periodontitis Wistar Rats Model with the Snail Slime (Achatina Fulica) Application

Cited by 6 publications

References 11 publications

The effect of the pseudostem extract of ambon banana on the number of lymphocytes and fibroblasts in gingivitis : an experimental study

The effect of the pseudostem extract of ambon banana on the number of lymphocytes and fibroblasts in gingivitis : an experimental study

Effectiveness of oral irrigation with an extract of green microalga Nannochloropsis oculata as an anti-inflammatory in rats infected with Aggregatibacter actinomycetemcomitans

<strong>The effect of brown algae (<em>Sargassum sp.</em>) gel on the number of osteoclasts in periodontitis rats</strong>

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