Alveolar epithelial damage. A critical difference between high pressure and oleic acid-induced low pressure pulmonary edema.

Montaner, Jsg; Tsang, John Y. C.; Evans, Ken; Mullen, J. B. M.; Burns, Alan R.; Walker, David C.; Wiggs, Barry; Hogg, James C.

doi:10.1172/jci112503

Cited by 60 publications

(31 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…4). Alveolar flooding may occur earlier because oleic acid has been shown recently to cause both an endothelial and epithelial injury (13). With an intact epithelial barrier, edema may accumulate in the interstitium, raising pressure, and effusions might occur more rapidly (13,14).…”

Section: Discussionmentioning

confidence: 99%

“…Alveolar flooding may occur earlier because oleic acid has been shown recently to cause both an endothelial and epithelial injury (13). With an intact epithelial barrier, edema may accumulate in the interstitium, raising pressure, and effusions might occur more rapidly (13,14). In separate experiments, we have studied the time course of pleural effusion formation in volume-overload pulmonary edema in sheep and found that the effusions do begin to accumulate within the first 1 h after the development of interstitial edema (15 ).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Relationship of pleural effusions to increased permeability pulmonary edema in anesthetized sheep.

Wiener-Kronish¹,

Broaddus²,

Albertine³

et al. 1988

J. Clin. Invest.

View full text Add to dashboard Cite

We studied anesthetized sheep to determine the relationship between increased permeability pulmonary edema and the development and mechanism of pleural effusion formation. In 12 sheep with intact, closed thoraces, we studied the time course of pleural liquid formation after 0.12 ml/kg i.v. oleic acid. After 1 h, there were no pleural effusions, even though extravascular lung water increased 50% to 6.0±0.7 g/g dry lung. By 3 h pleural effusions had formed, they reached a maximum at 5 h (48.5±16.9 ml/thorax), and at 8 h there was no additional accumulation of pleural liquid (45.5±16.9 ml). Morphologic studies by light and electron microscopy demonstrated subpleural edema but no detectable injury to the visceral pleura, suggesting that the pleural liquid originated from the lung and not the pleura. In nine sheep, we quantified the rate of formation of pleural liquid by enclosing one lung in a plastic bag. By comparing in the same sheep the volume of pleural liquid collected from the enclosed lung to the volume found in the opposite intact chest, we estimated the rate of liquid absorption from the intact chest to be 0.32 ml/(kg. h); we had previously reported a liquid absorption rate of 0.28 ml/(kg. h) in normal sheep. These studies also supported the conclusion that the majority of the pleural liquid originated from the lung because we could account for all of the pleural liquid that was formed and cleared. The volume of pleural liquid collected from the enclosed lungs was equal to 21% of the excess lung liquid that formed after oleic acid-induced lung injury. Thus, the pleural space and parietal pleural lymphatic pathways are important pathways for the clearance of pulmonary edema liquid after experimentally induced increased permeability pulmonary edema.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Relationship of pleural effusions to increased permeability pulmonary edema in anesthetized sheep.

Wiener-Kronish¹,

Broaddus²,

Albertine³

et al. 1988

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

“…Subtle but functionally important alterations would go undetected at the light microscopic level, e.g. only by electron microscopy is it possible to distinguish permeability oedema due to primary blood-air barrier injury in ALI from the lesions in hydrostatic oedema [36][37][38][39] and to analyse intra-alveolar surfactant subtype alterations [40][41][42][43]. Primary chemical fixation ''from behind'' by controlled vascular perfusion with a glutaraldehyde-containing fixative followed by a phospholipid-stabilising protocol is the method of choice when subsequent stereological analysis of oedema and surfactant has to be performed [44][45][46][47][48][49].…”

Section: Stereological Assessment Of Alimentioning

confidence: 99%

Stereological analysis of acute lung injury

Ochs¹

2006

Eur Respir Rev

View full text Add to dashboard Cite

Acute lung injury is associated with a variety of histopathological alterations, such as oedema formation, damage to the components of the blood-air barrier and impairment of the surfactant system. Stereological methods are indispensable tools with which to properly quantitate these structural alterations at the light and electron microscopic level. The stereological parameters that are relevant for the analysis of acute lung injury are reviewed in the present article.

show abstract

“…The alteration of fluid balance in the lung that results in pulmonary edema can be caused by increased hydrostatic pressure or increased permeability of the pulmonary capillary bed (Guyton and Lindsey, 1959;Gorin and Stewart, 1979;Montaner et al, 1986;Matthay et al, 2002). High-permeability pulmonary edema results from an increase in the vascular permeability of the lung, causing an accumulation of fluid and protein in the lung interstitium and air spaces (Gorin and Stewart, 1979;Montaner et al, 1986;Matthay et al, 2002). The increase in permeability can be initiated by a variety of factors, and is directly related to reversible physical modifications of the pulmonary endothelium (Fraser et al, 1999;Matthay and Martin, 2005;Leaver and Evans, 2007;Wheeler and Bernard, 2007).…”

Section: Introductionmentioning

confidence: 99%

Genetic Delivery of Bevacizumab to Suppress Vascular Endothelial Growth Factor-Induced High-Permeability Pulmonary Edema

2009

View full text Add to dashboard Cite

High-permeability pulmonary edema causing acute respiratory distress syndrome is associated with high mortality. Using a model of intratracheal adenovirus (Ad)-mediated overexpression of human vascular endothelial growth factor (VEGF)-A 165 in mouse lung to induce alveolar permeability and consequent pulmonary edema, we hypothesized that systemic administration of a second adenoviral vector expressing an anti-VEGF antibody (AdaVEGFAb) would protect the lung from pulmonary edema. Pulmonary edema was induced in mice by intratracheal administration of AdVEGFA165. To evaluate anti-VEGF antibody therapy, the mice were treated intravenously with AdaVEGFAb, an adenoviral vector encoding the light and heavy chains of an antihuman VEGF antibody with the bevacizumab (Avastin) antigen-binding site. Lung VEGF-A 165 and phosphorylated VEGF receptor (VEGFR)-2 levels, histology, lung wet-to-dry weight ratios, and bronchoalveolar lavage fluid (BALF) levels of total protein were assessed. Administration of AdaVEGFAb to mice decreased Ad-VEGFA165-induced levels of human VEGF-A 165 and phosphorylated VEGFR-2 in the lung. Histological analysis of AdaVEGFAb-treated mice demonstrated a reduction of edema fluid in the lung tissue that correlated with a reduction of lung wet-to-dry ratios and BALF total protein levels. Importantly, administration of AdaVEGFAb 48 hr after induction of pulmonary edema with AdVEGFA165 was effective in suppressing pulmonary edema. Administration of an adenoviral vector encoding an anti-VEGF antibody that is the equivalent of bevacizumab effectively suppresses VEGF-A 165 -induced high-permeability pulmonary edema, suggesting that anti-VEGF antibody therapy may represent a novel therapy for high-permeability pulmonary edema.

show abstract

Alveolar epithelial damage. A critical difference between high pressure and oleic acid-induced low pressure pulmonary edema.

Cited by 60 publications

References 24 publications

Relationship of pleural effusions to increased permeability pulmonary edema in anesthetized sheep.

Relationship of pleural effusions to increased permeability pulmonary edema in anesthetized sheep.

Stereological analysis of acute lung injury

Genetic Delivery of Bevacizumab to Suppress Vascular Endothelial Growth Factor-Induced High-Permeability Pulmonary Edema

Contact Info

Product

Resources

About