Alzheimerʼs paired helical filaments share epitopes with neurofilament side arms

Ccj., Miller; Jp, Brion; Calvert, R.; Chin, TK; Eagles, P.A.M.; Downes, MJ; Flament-Durand, J; Haugh, M; Kahn, J.; Probst, A.; Ulrich, Jason D.; Anderton, BH

doi:10.1097/00002093-198701040-00021

Cited by 2 publications

(2 citation statements)

References 36 publications

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“…Early studies also showed an imunolabelling of NFT by some anti-neurofilament antiserum [22,56,59], although the polyspecificity or the ill definition of the antigens was raised as a potential pitfall [37]. However, well-defined monoclonal antibodies to neurofilament polypeptides [4,85] and neurofilament antisera [37] were found to label NFT in situ and even isolated NFT [71,79]. At least some of these neurofilament antibodies were however later found to react also with tau proteins [64,76].…”

Section: Immunocytochemical Analysis Of Phfmentioning

confidence: 99%

Immunological demonstration of tau protein in neurofibrillary tangles of Alzheimer's disease

Brion

2006

JAD

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Neurofibrillary tangles are one of the neuropathological hallmark of Alzheimer's disease, described early as part of the pathological criteria of the disease. Ultrastuctural studies in the sixties showed their unusual features but their molecular composition was not unraveled before the mid-eighties. Initial biochemical studies suggested that they were composed of modified unidentified brain proteins, and several immunocytochemical studies suggested that they contained polypeptides cross-reactive with antibodies to cytoskeletal proteins. In 1985, we demonstrated that neurofibrillary tangles were immunolabelled by antibodies to the microtubule-associated protein tau and that antibodies raised to neurofibrillary tangles cross-reacted with tau proteins. These results were soon confirmed independently in several laboratories. Further studies were devoted to the analysis of tau post-translationnal modifications in the affected tissues and in cellular and animal models.

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Section: Immunocytochemical Analysis Of Phfmentioning

confidence: 99%

Immunological demonstration of tau protein in neurofibrillary tangles of Alzheimer's disease

Brion

2006

JAD

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“…A: anti-tau (Brion et al 1985a(Brion et al , 1988 B: anti-PHF (Brion et al 1985b;Miller et al 1986) C: Alz-50 (Wolozin et al 1986;Wolozin and Davies 1987;Tabaton et al 1988) D: Marsland (Marsland et al 1954), Congo red, Luxol fast blue, anti-RT97 E: Bielschowsky (Yamamoto and Hirano 1986), Bodian coupled with Luxol fast blue (Duyckaerts et al 1987) and Congo red (Putchler et al 1962(Putchler et al , 1965) F: Bielschowsky (Yamamoto and Hirano 1986) coupled with cresyl violet G: anti-ubiquitin (Lennox et al 1988) H: Cross (Cross 1982) I: Gallyas (Gallyas 1971) J: Bodian coupled with Luxol fast blue (Bodian 1936;Duyckaerts et al 1987) K: King's (Lloyd et al 1985, modified by G. Glover, Régional Neuropathology Service, Belfast) APPENDIX IB; STAINING TECHNIQUES FOR SENILE PLAQUES Observers who used différent techniques for senile plaques and neurofibrillary tangles, are in italics.…”

Section: The Difficulty Of Rating the Lightly Affected Casesmentioning

confidence: 99%

Rating of the lesions in senile dementia of the Alzheimer type: concordance between laboratories A European multicenter study under the auspices of EURAGE

Duyckaerts¹,

Delaère²,

Hauw³

et al. 1990

Journal of the Neurological Sciences

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The study reported was intended to compare the impressions and analyses of investigators from 11 different laboratories on 2 slides, each from 6 cases with varying quantities of neuropathological change of the type found in Alzheimer's disease and normal ageing. The material came from 6 selected female patients over 75 years of age all of whom had been examined in detail and assessed by the Blessed Test Score. Two were severely demented, 2 mildly demented and 2 were considered to be normal. Unstained paraffin-embedded slides were sent to the investigators, the choice of the staining techniques being left to each laboratory. A quantitative evaluation of the changes was requested in 2 specified areas of the hippocampus and in the first temporal gyrus. Subjective scores of severity and a final guess about the pre mortem intellectual status (demented or not) were asked. The 11 replies were analyzed. A total of 14 different staining techniques were used. Absolute values of density differed much from one investigator to another, for senile plaques as well as for neurofibrillary tangles. Statistical analysis showed that concordance might be improved by the use of corrective factors which would standardize the scales of measurement. The ranking of the slides in increasing order of severity was in good agreement for 9 out of 11 observers concerning the neurofibrillary tangles and 3 out of 9 observers concerning the senile plaques. The correlation between the intellectual status and the density of lesions was higher for neurofibrillary tangles than for senile plaques. The subjective scores were in better agreement for the severely affected cases than for the mildly affected ones. The lowest correlation with intellectual deficit was obtained with the quantitative scores which took into account only the senile plaques or only the hippocampal lesions. The highest correlation coefficients were obtained with the subjective scores. The observers guessed correctly the intellectual status of the 2 most affected cases and often disagreed for the intermediate and normal cases. Neuropathology is mandatory for the diagnosis of definite Alzheimer's disease. Quantitative assessment is useful in cases with few lesions and light dementia but the neuropathological diagnostic procedure has to be more strictly standardized before quantitative histopathological criteria can be reliably transferred from one laboratory to another, especially when mildly affected cases are involved. Concordance seems presently easier to obtain by ranking the lesions and the cases in increasing order of severity than by using quantitative values of density.(ABSTRACT TRUNCATED AT 400 WORDS)

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Alzheimerʼs paired helical filaments share epitopes with neurofilament side arms

Cited by 2 publications

References 36 publications

Immunological demonstration of tau protein in neurofibrillary tangles of Alzheimer's disease

Immunological demonstration of tau protein in neurofibrillary tangles of Alzheimer's disease

Rating of the lesions in senile dementia of the Alzheimer type: concordance between laboratories A European multicenter study under the auspices of EURAGE

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