Amanitacinis and A. olivovaginata (Basidiomycota, Amanitaceae), two new species, and the first record of A. emodotrygon, from Northwestern Pakistan

Abstract

Two new species found in Northwestern Pakistan in the mushroom genus Amanita are described and illustrated. Phylogenetic data derived from nuclear ribosomal ITS and LSU regions along with morphological characterizations indicate that these species are novel. Amanita cinis is a member of section Roanokenses, while A. olivovaginata is representative of the section Vaginatae. Amanita emodotrygon, recently described from the state of Uttarakhand, India, is new to Pakistan.

Veloporphyrellus latisporus (Boletaceae), a new species from moist temperate forests of Pakistan

“…Hook., Acer caesium Wall. ex Brandis, Juglans regia L., Quercus incana Roxb., Q. semicarpifolia Smith and Ulmus wallichiana Planch (Ullah et al 2019).…”

Russulashanglaensis sp. nov. (Basidiomycota: Russulales), a new species from the mixed coniferous forests in District Shangla, Pakistan

“…Polymerase chain reaction (PCR) and cycle sequencing were performed to obtain sequences of nuc-rDNA internal transcribed spacer region (ITS) using primer pairs ITS1F/ ITS4 (White et al, 1990;Gardes and Bruns, 1993). For PCR conditions, we followed Ullah et al (2019aUllah et al ( , 2019c. DNA extraction, PCR, and cycle sequencing reactions were performed at the Chicago Botanic Garden Negaunee Institute for Plant Conservation Science and Action, Glencoe, Illinois.…”

“…Phylogenetic analyses were conducted using Bayesian and maximum likelihood (ML) methods. Bayesian analysis was undertaken using BEAST 1.8.2 (Drummond and Rambaut, 2007) following Ullah et al (2019c). Maximum likelihood analyses were run in RAxML-II-HPC (Stamatakis, 2006).…”

“…mixed forests, which provide an ideal habitat for a large number of mycorrhizal fungi. Due to the unique geographic position and rich floristic composition, the area is considered a hot spot of fungal diversity (Ullah et al, 2019c).…”

Two new species of Amanita section Roanokenses with a radicating basal bulb

“…Amanita is the largest genus; it harbors 95% of the species in the family and includes more than 600 accepted species (Kirk et al 2008;Cui et al 2018). Many species of Amanitaceae have been extensively collected and studied since the 1990s in China, and there are more than 200 formally described taxa (Yang 1997(Yang , 2005(Yang , 2015Zhang et al 2010;Deng et al 2014;Deng et al 2016;Li and Cai 2014;Ariyawansa et al 2015;Li et al 2015;Cai et al 2016;Liu et al 2017;Cui et al 2018;Jabeen et al 2019;Ullah et al 2019;Saba et al 2019;Zhang et al 2020).…”