Amantadine resistance markers among low pathogenic avian influenza H9N2 viruses isolated from poultry in India, during 2009–2017

Kode, Sadhana S.; Pawar, Shailesh D.; Tare, Deeksha S.; Keng, Sachin S.; Mullick, Jayati

doi:10.1016/j.micpath.2019.103779

Cited by 12 publications

(5 citation statements)

References 22 publications

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“…During this stage, the HA protein of the virus remains bound to sialic acid on the host cells, and the function of the viral NA protein is required to cleave this bond, allowing the virus to detach from the cells completely. (Sriwilaijaroen & Suzuki 2012;Kode et al 2019) In this study, NanB sialidase developed as an antiviral agent to inhibit the initial and nal stages of H9N2, thereby impeding the virus infection process.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Antiviral Activity of NanB Bacterial Sialidase Against Avian Influenza H9N2 Virus in MDCK cells

Nugroho

Silaen

Kurnia³

et al. 2023

Preprint

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Avian influenza virus are infectious agent that may cause global health problem issues in poultry and potentially zoonotic. The high risk of circulating antiviral agents due to genetic mutations is a concern, highlighting the need for development of new antiviral agents. In this study the antiviral activity of NanB sialidase from P. multocida was investigated through in vitro analysis using MDCK cells. NanB sialidase were purified from P. multocida for testing its toxicity and its ability to hydrolyze its sialic acid receptors on MDCK cells. H9N2 challenge virus was adapted in MDCK cells until cytopathic effects (CPE) appeared. Antiviral activity of NanB sialidase was conducted using MDCK cells, and then observed based on cell morphology, viral copy number, and expression of apoptosis-mediating genes. NanB sialidase effectively hydrolyze Neu5Acα(2–6)Gal sialic acid at the dose of 129 mU/ml, while at 258 mU/ml it cause toxicity on MDCK cells. Antiviral activity of sialidase is evident based on the significantly decrease in viral copy number at all doses administrated. The increase of p53 and caspase-3 expression was observed in infected cells without sialidase and highest dose of 258 mU/ml sialidase, indicating the occurrence of apoptosis mechanism. Our study demonstrates the ability of NanB sialidase to inhibit H9N2 virus replication based on observations of sialic acid hydrolysis, reduction in viral copy number, and expression of apoptosis-related genes. The future application of sialidase may be considered as an antiviral strategy against avian influenza H9N2 virus infections.

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Section: Discussionmentioning

confidence: 99%

In Vitro Antiviral Activity of NanB Bacterial Sialidase Against Avian Influenza H9N2 Virus in MDCK cells

Nugroho

Silaen

Kurnia³

et al. 2023

Preprint

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“…Moreover, given the time-intensive nature of vaccine production, which typically spans a minimum of six months, ATVs emerge as a valuable adjunct to complement the preventive strategies. Two distinct classes of antiviral agents, namely adamantanes (amantadine and rimantadine) and neuraminidase inhibitors (NAIs) (oseltamivir and zanamivir), have emerged as therapeutic options for the management of influenza infection [ 53 ].…”

Section: Types Of Atvsmentioning

confidence: 99%

Recent Developments in Semiconductor-Based Photocatalytic Degradation of Antiviral Drug Pollutants

Zhang

Zhao

et al. 2023

Toxics

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The prevalence of antiviral drugs (ATVs) has seen a substantial increase in response to the COVID-19 pandemic, leading to heightened concentrations of these pharmaceuticals in wastewater systems. The hydrophilic nature of ATVs has been identified as a significant factor contributing to the low degradation efficiency observed in wastewater treatment plants. This characteristic often necessitates the implementation of additional treatment steps to achieve the complete degradation of ATVs. Semiconductor-based photocatalysis has garnered considerable attention due to its promising potential in achieving efficient degradation rates and subsequent mineralization of pollutants, leveraging the inexhaustible energy of sunlight. However, in recent years, there have been few comprehensive reports that have thoroughly summarized and analyzed the application of photocatalysis for the removal of ATVs. This review commences by summarizing the types and occurrence of ATVs. Furthermore, it places a significant emphasis on delivering a comprehensive summary and analysis of the characteristics pertaining to the photocatalytic elimination of ATVs, utilizing semiconductor photocatalysts such as metal oxides, doped metal oxides, and heterojunctions. Ultimately, the review sheds light on the identified research gaps and key concerns, offering invaluable insights to steer future investigations in this field.

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“…In addition, since the production of the vaccine takes at least 6 months, antiviral drugs become a complement to vaccines [36] . Two groups of anti-influenza drugs, adamantanes (amantadine and rimantadine) and neuraminidase inhibitors (NAI) (oseltamivir and zanamivir) are used for the treatment of influenza infectious disease [33] , [37] .…”

Section: Antiviral Drugs and Virusmentioning

confidence: 99%

A sustainable approach for the removal methods and analytical determination methods of antiviral drugs from water/wastewater: A review

Eryıldız

Gül

Koyuncu

2022

Journal of Water Process Engineering

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Amantadine resistance markers among low pathogenic avian influenza H9N2 viruses isolated from poultry in India, during 2009–2017

Cited by 12 publications

References 22 publications

In Vitro Antiviral Activity of NanB Bacterial Sialidase Against Avian Influenza H9N2 Virus in MDCK cells

In Vitro Antiviral Activity of NanB Bacterial Sialidase Against Avian Influenza H9N2 Virus in MDCK cells

Recent Developments in Semiconductor-Based Photocatalytic Degradation of Antiviral Drug Pollutants

A sustainable approach for the removal methods and analytical determination methods of antiviral drugs from water/wastewater: A review

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