Amatoxins and phallotoxins in indigenous and introduced South African Amanita species

Hallen, Heather E.; Adams, G.C.; Eicker, A.

doi:10.1016/s0254-6299(15)30393-8

Cited by 25 publications

(23 citation statements)

References 10 publications

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“…et al was observed in association with exotic trees of Myrtaceae, Casuarinaceae and Araucariaceae [7]. Hallen et al [29] suggested that A. reidii might be synonymous with the Australian species A. marmorata subsp. marmorata Cleland & E.-J.…”

Section: Discussionmentioning

confidence: 99%

Multi-locus phylogeny of lethal amanitas: Implications for species diversity and historical biogeography

et al. 2014

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Background: Lethal amanitas (Amanita section Phalloideae) are a group of wild, fatal mushrooms causing many poisoning cases worldwide. However, the diversity and evolutionary history of these lethal mushrooms remain poorly known due to the limited sampling and insufficient gene fragments employed for phylogenetic analyses. In this study, five gene loci (nrLSU, ITS, rpb2, ef1-α and β-tubulin) with a widely geographic sampling from East and South Asia, Europe, North and Central America, South Africa and Australia were analysed with maximum-likelihood, maximum-parsimony and Bayesian inference methods. Biochemical analyses were also conducted with intention to detect amatoxins and phalloidin in 14 representative samples.

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Section: Discussionmentioning

confidence: 99%

Multi-locus phylogeny of lethal amanitas: Implications for species diversity and historical biogeography

et al. 2014

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“…A third possible example of horizontal gene transfer is the bicyclic peptide amatoxins (amanitin) and phallotoxins (phalloidin). Certain species in several dispersed genera within the Agaricales (Basidiomycota), including Amanita, Galerina , Conocybe, and Lepiota , make amatoxins, or phallotoxins, or both, whereas other species in these genera and in the intervening taxa do not (Hallen et al, 2002). Whether this occurred by common descent or by horizontal transfer cannot be addressed until at least one of the responsible biosynthetic genes has been isolated.…”

Section: Evolution Of Secondary Metabolite Pathwaysmentioning

confidence: 99%

Peptide Synthesis without Ribosomes

Walton

Panaccione

Hallen

2004

Advances in Fungal Biotechnology for Industry, Agriculture, and Medicine

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“…To the best of the authors' knowledge, all published procedures focus on extraction of only the polar cyclopeptides by incubating macerated mushroom tissue for 12–24 hr in aqueous‐methanolic solutions with the addition of mineral or organic acid (e.g., hydrochloric or formic acid). Some protocols (Deng et al, 2011; Hallen, Adams, & Eicker, 2002) call for re‐extraction of the remaining pellet, extending the procedure to 48 hr. We compared this procedure to the ASE protocol and demonstrated that this “Classic” approach was not exhaustive even following double extraction of the mushroom samples.…”

Section: Discussionmentioning

confidence: 99%

Exhaustive extraction of cyclopeptides from Amanita phalloides: Guidelines for working with complex mixtures of secondary metabolites

Chialvo

Griffin

Reed

et al. 2020

Ecology and Evolution

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Understanding plant‐insect interactions is an active area of research in both ecology and evolution. Much attention has been focused on the impact of secondary metabolites in the host plant or fungi on these interactions. Plants and fungi contain a variety of biologically active compounds, and the secondary metabolite profile can vary significantly between individual samples. However, many experiments characterize the biological effects of only a single secondary metabolite or a subset of these compounds. Here, we develop an exhaustive extraction protocol using an accelerated solvent extraction protocol to recover the complete suite of cyclopeptides and other secondary metabolites found in Amanita phalloides (death cap mushrooms) and compare its efficacy to the “Classic” extraction method used in earlier works. We demonstrate that our extraction protocol recovers the full suite of cyclopeptides and other secondary metabolites in A. phalloides unlike the “Classic” method that favors polar cyclopeptides. Based on these findings, we provide recommendations for how to optimize protocols to ensure exhaustive extracts and also the best practices when using natural extracts in ecological experiments.

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Amatoxins and phallotoxins in indigenous and introduced South African Amanita species

Cited by 25 publications

References 10 publications

Multi-locus phylogeny of lethal amanitas: Implications for species diversity and historical biogeography

Multi-locus phylogeny of lethal amanitas: Implications for species diversity and historical biogeography

Peptide Synthesis without Ribosomes

Exhaustive extraction of cyclopeptides from Amanita phalloides: Guidelines for working with complex mixtures of secondary metabolites

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