Ambient Sampling/Ionization Mass Spectrometry: Applications and Current Trends

Harris, Geoffrey; Galhena, Asiri S.; Fernández, Facundo M.

doi:10.1021/ac200918u

Cited by 490 publications

(394 citation statements)

References 220 publications

Supporting

Mentioning

392

Contrasting

Order By: Relevance

“…Now, desorption electrospray ionization mass spectrometry (DESI-MS) (11), as well as related MS-imaging methods (12), offer the possibility of incorporating lipid information into tumor diagnostics. DESI is one of a recently developed group of ionization techniques in MS in which samples are examined in the ambient environment with minimal pretreatment (13)(14)(15)(16)(17). Using DESI, direct lipid analysis from biological samples such as tissue sections can be performed rapidly and routinely.…”

mentioning

confidence: 99%

Ambient mass spectrometry for the intraoperative molecular diagnosis of human brain tumors

Eberlin

Norton²,

Orringer³

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

263

234

View full text Add to dashboard Cite

The main goal of brain tumor surgery is to maximize tumor resection while preserving brain function. However, existing imaging and surgical techniques do not offer the molecular information needed to delineate tumor boundaries. We have developed a system to rapidly analyze and classify brain tumors based on lipid information acquired by desorption electrospray ionization mass spectrometry (DESI-MS). In this study, a classifier was built to discriminate gliomas and meningiomas based on 36 glioma and 19 meningioma samples. The classifier was tested and results were validated for intraoperative use by analyzing and diagnosing tissue sections from 32 surgical specimens obtained from five research subjects who underwent brain tumor resection. The samples analyzed included oligodendroglioma, astrocytoma, and meningioma tumors of different histological grades and tumor cell concentrations. The molecular diagnosis derived from mass-spectrometry imaging corresponded to histopathology diagnosis with very few exceptions. Our work demonstrates that DESI-MS technology has the potential to identify the histology type of brain tumors. It provides information on glioma grade and, most importantly, may help define tumor margins by measuring the tumor cell concentration in a specimen. Results for stereotactically registered samples were correlated to preoperative MRI through neuronavigation, and visualized over segmented 3D MRI tumor volume reconstruction. Our findings demonstrate the potential of ambient mass spectrometry to guide brain tumor surgery by providing rapid diagnosis, and tumor margin assessment in near-real time.

show abstract

mentioning

confidence: 99%

Ambient mass spectrometry for the intraoperative molecular diagnosis of human brain tumors

Eberlin

Norton²,

Orringer³

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

263

234

View full text Add to dashboard Cite

show abstract

“…DESI-MSI is an ambient ionization technique in mass spectrometry (20,21) that has recently emerged for imaging biological samples without the need of extensive sample preparation. DESI-MSI has been particularly powerful for investigating the distribution of diagnostic lipids and metabolites in a variety of human cancers directly from tissue sections (22)(23)(24).…”

mentioning

confidence: 99%

Alteration of the lipid profile in lymphomas induced by MYC overexpression

Eberlin

Gabay

Fan

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

121

103

View full text Add to dashboard Cite

Overexpression of the v-myc avian myelocytomatosis viral oncogene homolog (MYC) oncogene is one of the most commonly implicated causes of human tumorigenesis. MYC is known to regulate many aspects of cellular biology including glucose and glutamine metabolism. Little is known about the relationship between MYC and the appearance and disappearance of specific lipid species. We use desorption electrospray ionization mass spectrometry imaging (DESI-MSI), statistical analysis, and conditional transgenic animal models and cell samples to investigate changes in lipid profiles in MYC-induced lymphoma. We have detected a lipid signature distinct from that observed in normal tissue and in rat sarcoma-induced lymphoma cells. We found 104 distinct molecular ions that have an altered abundance in MYC lymphoma compared with normal control tissue by statistical analysis with a false discovery rate of less than 5%. Of these, 86 molecular ions were specifically identified as complex phospholipids. To evaluate whether the lipid signature could also be observed in human tissue, we examined 15 human lymphoma samples with varying expression levels of MYC oncoprotein. Distinct lipid profiles in lymphomas with high and low MYC expression were observed, including many of the lipid species identified as significant for MYC-induced animal lymphoma tissue. Our results suggest a relationship between the appearance of specific lipid species and the overexpression of MYC in lymphomas.biostatistics | transgenic models | lipidomics | metabolomics | cancer T he v-myc avian myelocytomatosis viral oncogene homolog (MYC) is commonly overexpressed in human neoplasia (1, 2) and has been strongly associated with the clinical aggressiveness of human cancers (3, 4). MYC is particularly associated with the pathogenesis of hematopoietic tumors such as lymphomas and of epithelial tumors (5, 6). In Burkitt's lymphoma, for example, the c-Myc gene is translocated to one of the Ig loci in virtually all tumors (5,7,8). The MYC oncogene contributes to tumorigenesis by functioning as a global regulator of transcription involving many cellular programs, including cellular growth, metabolism, and lipid synthesis (9, 10). MYC induces a global shift in metabolism associated with anaerobic glycolysis, a phenomenon known as the Warburg effect (11). Several studies have shown a relationship between MYC regulation and metabolism in lymphomas, especially as related to the processes of glycolysis and glutaminolysis (12, 13). Some reports suggest that MYC regulates fatty acid synthesis, specifically palmitate (14); however, little is known about the relationship between MYC expression and the up-regulation or down-regulation of various lipid species in lymphomas.We have generated transgenic mice to conditionally regulate expression of MYC oncogenes alone or in combination with oncogenes such as rat sarcoma (RAS), BCR-ABL, and BCL2 to produce many transgenic models of cancers, including T-cell acute lymphocytic leukemia, acute myeloid leukemia, osteosarcoma, lung adenocarc...

show abstract

“…So, researchers mainly focused attention on high-throughput screening of target compounds, and several reviews had summarized these advances. [16][17][18][19] Here we merely concentrate on new trends of DART-MS, like fingerprint, reaction monitor, imaging and so on.…”

Section: Applications Of Dart-msmentioning

confidence: 99%

Direct Analysis in Real Time Mass Spectrometry: a Powerful Tool for Fast Analysis

Li¹,

Wang²,

Li³

et al. 2015

Mass Spectrometry Letters

View full text Add to dashboard Cite

Direct analysis in real time mass spectrometry (DART-MS) is one of the variants of ambient mass spectrometry. The ionization process of DART-MS is in open environment and only takes few seconds, so it is suitable for fast analysis. Actually, since its introduction in 2005, more and more attentions have been drawn to its various applications due to its excellent properties, e.g., fast analysis, and no or less sample preparation, high salt tolerance and so on. This review summarized the promising features of DART-MS, including its ionization mechanism, equipment modification, wide applications, coupling techniques and extraction strategies before analysis.

show abstract

Ambient Sampling/Ionization Mass Spectrometry: Applications and Current Trends

Cited by 490 publications

References 220 publications

Ambient mass spectrometry for the intraoperative molecular diagnosis of human brain tumors

Ambient mass spectrometry for the intraoperative molecular diagnosis of human brain tumors

Alteration of the lipid profile in lymphomas induced by MYC overexpression

Direct Analysis in Real Time Mass Spectrometry: a Powerful Tool for Fast Analysis

Contact Info

Product

Resources

About