Ambient temperature storage of tissue samples (bovine) in readily available media during mass fatality incidents

Chan, Xavier Liang Shun; Kua, Guo Wei; Lai, Shu‐Mei ‐M; Lim, Holden Wei Siong; Wee, Ming Xue; Syn, Christopher Kiu Choong

doi:10.1016/j.fsigss.2019.09.087

Cited by 2 publications

(2 citation statements)

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“…For example, alcohol in the form of drinking alcohol and as a disinfectant can be easily acquired from supermarkets/grocery stores in Singapore. Other studies have also been performed on the use of drinking liquors on tissue preservation [ 4 , 14 ]. Such readily available chemicals may be of particular relevance in terms of preserving soft tissue samples of victims for subsequent DNA-based identification in national emergencies where there may be prolonged disruption to electrical power supply.…”

Section: Introductionmentioning

confidence: 99%

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Long-Term Tissue Preservation at Ambient Temperature for Post-Mass Fatality Incident DNA-Based Victim Identification

Chan,

Lai,

bin Hamdan

et al. 2024

Genes

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In a mass fatality incident (MFI), effective preservation of tissue samples is the cornerstone for downstream DNA-based identification of victims. This is commonly achieved through freezing of tissue samples excised from bodies/fragmented remains which may be buried or stored in refrigerated containers. This may, however, not be possible depending on the nature of the MFI; in particular, during armed conflict/war where extended periods of electrical outages would be expected. The present study compared the effectiveness of long-term tissue preservation at ambient temperatures using two commercial products (non-iodized kitchen salt and a 40% alcoholic beverage) against a chemical preservative (Allprotect™ Tissue Reagent (Qiagen, Germantown, MD, USA)) and freezing at −20 °C. Bovine muscle tissue, used as a proxy for human tissue, was treated with the four preservation methods and sampled at six different time-points over a 24-month period. All four methods were able to preserve the bovine tissue, generally yielding STR-PCR (Short Tandem Repeat-Polymerase Chain Reaction) amplicons > 200 bp in size even at the end of 24 months. Gel electrophoresis, however, indicated that salt was more effective in preserving DNA integrity with high-molecular-weight DNA clearly visible as compared to the low-molecular-weight DNA smears observed in the other methods. This study also proposes a simple process for the rapid and low-cost preservation of tissue samples for long-term storage at ambient temperatures in support of post-incident victim identification efforts.

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Section: Introductionmentioning

confidence: 99%

“…The previous literature has reported successful tissue preservation (up to 1 year) using a readily available household chemical: kitchen salt [4][5][6][7][8][9]. Kitchen salt is easily available Genes 2024, 15, 373 2 of 12 and usually affordable in most parts of the world.…”

Section: Introductionmentioning

confidence: 99%