Ameliorating Role of Rutin on Oxidative Stress Induced by Iron Overload in Hepatic Tissue of Rats

Abstract

Iron is an essential element that participates in several metabolic activities of cells; however, excess iron is a major cause of iron-induced oxidative stress and several human diseases. Natural flavonoids, as rutin, are well-known antioxidants and could be efficient protective agents. Therefore, the present study was undertaken to evaluate the protective influence of rutin supplementation to improve rat antioxidant systems against IOL-induced hepatic oxidative stress. Sixty male albino rats were randomly divided to three equal groups. The first group, the control, the second group, iron overload group, the third group was used as iron overload+rutin group. Rats received six doses of ferric hydroxide polymaltose (100 mg kg(-1) b.wt.) as one dose every two days, by intraperitoneal injections (IP) and administrated rutin (50 mg kg(-1) b.wt.) as one daily oral dose until the sacrificed day. Blood samples for serum separation and liver tissue specimens were collected three times, after three, four and five weeks from the onset of the experiment. Serum iron profiles total iron, Total Iron Binding Capacity (TIBC), Unsaturated Iron Binding Capacity (UIBC), transferrin (Tf) and Transferrin Saturation% (TS%)}, ferritin, albumin, total Protein, total cholesterol, triacylglycerols levels and aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities were determined. Moreover, total iron in the liver, L-malondialdehyde (L-MDA), glutathione (GSH), Nitric Oxide (NO) and Total Nucleic Acid (TNA) levels and glutathione peroxidase (GPx), catalase (CAT) and superoxide dismutase (SOD) activities were also determined. The obtained results revealed that, iron overload (IOL) resulted in significant increase in serum iron, TIBC, Tf, TS% and ferritin levels and AST and ALT activities and also increased liver iron, L-MDA and NO levels. Meanwhile, it decreased serum UIBC, total cholesterol, triacylglycerols, albumin, total protein and liver GSH, TNA levels and Gpx, CAT and SOD activities when compared with the control group. Rutin administration to iron-overloaded rats resulted in significant decrease in serum total iron, TIBC, Tf, TS%, ferritin levels and AST and ALT activities and liver total iron, L-MDA and NO levels with significant increases in serum UIBC, albumin, total protein and total cholesterol levels and in liver GSH, CAT and SOD activities compared with the IOL group. This study provides in vivo evidence that rutin administration can improve the antioxidant defense systems against IOL-induced hepatic oxidative stress in rats. This protective effect in liver of iron-loaded rats may be due to both antioxidant and metal chelation activities.

The protective mechanism of Nigella sativa against diethylnitrosamine‐induced hepatocellular carcinoma through its antioxidant effect and EGFR/ERK1/2 signaling

“…65,66 Rutin and phenolic acids increase antioxidant enzymes activities in several studies. 67,68 Silymarinprotected group showed the significant elevation in SOD, GST, GPx, and CAT activities revealing its antioxidant activity. 56,69 This may be due to its phenolic nature, which makes it able to donate electrons to stabilize free radicals and ROS leading to an enhanced DENA detoxification, elimination and reduction of carcinogen-DNA adducts formation.…”

The Protective Effects of Hydro-Alcoholic Extract of Coriandrum sativum in Rats with Experimental Iron-Overload Condition

“…Results reported by Gavin et al and Shengjiang Guan et al indicated dysfunction of heart and kidney in iron overload (2,33). In the current study, after injection of iron dextran, kidney histological damage was observed, yet treatJundishapur J Nat Pharm Prod.…”

“…The role of iron in the development of hepatic injuries in several clinical and experimental conditions has commonly been studied by induction of an iron overload condition (29)(30)(31)(32)(33).…”

Fenugreek (Trigonellafoenum-Graecum L) Seed Phytochemicals Protect DNA from Free Radical Induced Oxidative Damage and Inhibit Dipeptidyl Peptidase-IV an Enzyme Associated with Hyperglycemia

“…These include positions between the 4-oxo and the 3-or 5-OH groups that can form complexes with a binding stoichiometry of 1:1 or 1:2, metal/myricetin [43,44]. Similarly, a glycoside, rutin ameliorated oxidative stress induced by iron overloaded in hepatic tissue of rats [45]. It is to be noted that myricetin and rutin were the major flavonoids detected in fenugreek seeds ( Table 1).…”