Amelioration of collagen-induced arthritis by human recombinant soluble FcγRIIb

Magnusson, Sofia; Andrén, Maria; Nilsson, Kajsa E.; Sondermann, Peter; Jacob, Uwe; Kleinau, Sandra

doi:10.1016/j.clim.2008.02.002

Cited by 38 publications

(23 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The measurement of anti-CII antibodies was done using ELISA as previously described. 28 Briefly, 96-well MaxiSorp plates (NuncBrand Thermo Fischer Scientific, Roskilde, Denmark) were coated over night at 4 uC with BCII, MCII, or bovine serum albumin (BSA) fraction V (Merck KGaA, Darmstadt, Germany) and subsequently blocked with BSA followed by addition of the isolated serum, diluted 325 or 3100 for CII-specific IgM or IgG detection, respectively. For detection of antigen-specific IgM and IgG, we used alkaline phosphatase-conjugated sheep anti-mouse IgM or IgG (Sigma-Aldrich).…”

Section: Immunizationmentioning

confidence: 99%

Function and regulation of self-reactive marginal zone B cells in autoimmune arthritis

et al. 2015

Self Cite

View full text Add to dashboard Cite

Polyreactive innate-type B cells account for many B cells expressing self-reactivity in the periphery. Improper regulation of these B cells may be an important factor that underlies autoimmune disease. Here we have explored the influence of self-reactive innate B cells in the development of collagen-induced arthritis (CIA), a mouse model of rheumatoid arthritis. We show that splenic marginal zone (MZ), but not B-1 B cells exhibit spontaneous IgM reactivity to autologous collagen II in naïve mice. Upon immunization with heterologous collagen II in complete Freund's adjuvant the collagen-reactive MZ B cells expanded rapidly, while the B-1 B cells showed a modest anti-collagen response. The MZ B cells were easily activated by toll-like receptor (TLR) 4 and 9-ligands in vitro, inducing proliferation and cytokine secretion, implying that dual engagement of the B-cell receptor and TLRs may promote the immune response to self-antigen. Furthermore, collagen-primed MZ B cells showed significant antigen-presenting capacity as reflected by cognate T-cell proliferation in vitro and induction of IgG anti-collagen antibodies in vivo. MZ B cells that were deficient in complement receptors 1 and 2 demonstrated increased proliferation and cytokine production, while Fcc receptor IIb deficiency of the cells lead to increased cytokine production and antigen presentation. In conclusion, our data highlight self-reactive MZ B cells as initiators of the autoimmune response in CIA, where complement and Fc receptors are relevant in controlling the self-reactivity in the cells.

show abstract

Section: Immunizationmentioning

confidence: 99%

Function and regulation of self-reactive marginal zone B cells in autoimmune arthritis

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…In murine models, infusion of recombinant soluble FcγRIIa and FcγRIIIa can inhibit immune complex triggered inflammation [40]. Synthesized soluble FcγRIa and FcγRIIb have also been shown to absorb pathogenic immune complexes and to reduce disease severity in murine arthritis models [41–43]. Of note, other than serving as decoy receptors for immune complexes, there may be additional pro- or anti-inflammatory effects caused by circulating FcγRs, such as interacting with non-Ig-ligands and inducing the production of IL-12 [44, 45].…”

Section: Strategies For Targeting Fcrs In Autoimmune Diseasesmentioning

confidence: 99%

Targeting the Fc receptor in autoimmune disease

Kimberly

2014

Expert Opinion on Therapeutic Targets

View full text Add to dashboard Cite

Introduction The Fc receptors and their interaction with immunoglobulin and innate immune opsonins such as CRP are key players in humoral and cellular immune responses. As the effector mechanism for some therapeutic monoclonal antibodies and often a contributor to the pathogenesis and progression of autoimmunity, FcRs are promising targets for treating autoimmune diseases. Areas covered This review discusses the nature of different Fc receptors and the various mechanisms of their involvement in initiating and modulating immunocyte functions and their biological consequences. It describes a range of current strategies in targeting Fc receptors and manipulating their interaction with specific ligands while presenting the pros and cons of these approaches. This review also discusses potential new strategies including regulation of FcR expression and receptor cross-talk. Expert opinion Fc receptors are appealing targets in the treatment of inflammatory autoimmune diseases. However, there are still knowledge limitations and technical challenges, the most important being a better understanding of the individual roles of each of the Fc receptors and enhancement of the specificity in targeting particular cell types and specific Fc receptors.

show abstract

“…The CIIimmunized mice were inspected 2-3 times a week for arthritis development, starting 3 weeks after immunization, and clinical severity of arthritis was graded, as described previously. 23 …”

Section: Collagen Preparationmentioning

confidence: 99%

“…23 Briefly, sera from CII-or OVA-immunized mice were added to 96-well plates (MaxiSorp; NuncBrand Thermo Fischer Scientific, Roskilde, Denmark), which were coated with BCII and OVA, respectively. After incubation, alkaline phosphatase-conjugated sheep anti-mouse IgM or IgG Abs (Sigma) were added for total Ig measurement and biotin-conjugated goat anti-mouse IgG1, IgG2a, IgG2b and IgG3 Abs (Southern Biotech, Birmingham, AL, USA) for subclass measurement.…”

Section: Elisamentioning

confidence: 99%