Amelioration of inflammation, angiogenesis and CTGF expression in an arthritis model by a TSP1‐derived peptide treatment

Rico, Mario C.; Castaneda, Julian L.; Manns, Joanne M.; Uknis, Audrey B.; Sáinz, Irma M.; Safadi, Fayez F.; Popoff, Steve N.; Cadena, Raul A. DeLa

doi:10.1002/jcp.20958

Cited by 33 publications

(26 citation statements)

References 37 publications

(55 reference statements)

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“…This report is the first to show that ICs in the sera of RA patients contain TSP-1, and the IC may be a promising marker for RA. Recent studies have suggested that the TSP-1/ transforming growth factor/connective tissue growth factor pathway plays an important role in angiogenesis and erosive arthritis lesions (13,14 ). Although platelet factor 4 (PF4) was less sensitive (52.4%; 95% CI, 30%-75%) than TSP-1, our method also specifically detected PF4 or a variant incorporated into CICs in RA patients for the first time (Table 1, Fig.…”

Section: Discussionmentioning

confidence: 81%

Immune Complexome Analysis of Serum and Its Application in Screening for Immune Complex Antigens in Rheumatoid Arthritis

et al. 2011

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BACKGROUND Analysis of circulating immune complexes (CICs) produced during an immune response may be useful in elucidating some aspects of this process. Identification of antigens incorporated into CICs provides information that may be helpful in developing diagnostic and treatment strategies for autoimmune diseases, infection, cancer, and transplantation therapy, and such information might be more relevant than information on free antigens. Because CICs may contain many antigens, comprehensive identification and profiling of such antigens is more effective than immunoblotting detection. METHODS We developed a novel proteomic strategy (immune complexome analysis) in which immune complexes (ICs) are separated from serum, digested directly with trypsin, and then subjected to nano-liquid chromatography–tandem mass spectrometry for identifying and profiling antigens in CICs. We applied this strategy to the analysis of CICs in 21 rheumatoid arthritis (RA) patients. Serum samples from 13 healthy donors and 8 osteoarthritis patients were used as controls. RESULTS CICs containing thrombospondin-1 (TSP-1) and platelet factor 4 (PF4) were found in the serum of 81% and 52% of RA patients, respectively, and in none of the controls. CONCLUSIONS The ICs in the serum of a majority of the RA patients contained TSP-1 or PF4, and these ICs may have potential as alternative biomarkers. Our technique for immune complexome analysis uses routine clinical samples, simple protocols, and widely available equipment. This method may be generally applicable to the study of the relationship between CICs and certain diseases associated with the immune response in animals and humans.

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Section: Discussionmentioning

confidence: 81%

Immune Complexome Analysis of Serum and Its Application in Screening for Immune Complex Antigens in Rheumatoid Arthritis

et al. 2011

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“…The role of TSP-1 in RA and angiogenesis is described above [50]. In a recent study [50], a TSP-1-derived peptide suppressed synovial inflammation and angiogenesis in a rat arthritis model.…”

Section: Further Advances In Angiostatic Therapymentioning

confidence: 96%

“…In a recent study [50], a TSP-1-derived peptide suppressed synovial inflammation and angiogenesis in a rat arthritis model.…”

Section: Further Advances In Angiostatic Therapymentioning

confidence: 98%

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Angiogenesis and vasculogenesis in rheumatoid arthritis

Szekanecz

Besenyei

Simonyi

et al. 2010

Current Opinion in Rheumatology

129

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“…These properties might be due in part to specific secreted factors, including some types of cytokines and growth factors. For instance, it has been reported that thrombospondin-1, 2 (TSP-1, 2) functions as an anti-inflammatory factor in RA by suppressing production of proinflammatory mediators such as IFN-γ and TNF-α, inducing depletion of synovium residing T cells and reducing infiltration of monocytes/macrophages in articular tissues [18,19] . In this fashion, chondrogenic differentiation and paracrine actions might be involved in replacement of damaged cartilage tissues and stimulation of the regeneration process.…”

Section: Hucb-mscs For Cartilage Regenerationmentioning

confidence: 99%

Application of human umbilical cord blood-derived mesenchymal stem cells in disease models

Kim¹

2010

WJSC

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Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) are regarded as an alternative source of bone marrow-derived mesenchymal stem cells because collection of cord blood is less invasive than that of bone marrow. hUCB-MSCs have recently been studied for evaluation of their potential as a source of cell therapy. In this review, the general characteristics of hUCB-MSCs and their therapeutic effects on various diseases in vitro and in vivo will be discussed.

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Amelioration of inflammation, angiogenesis and CTGF expression in an arthritis model by a TSP1‐derived peptide treatment

Abstract: Both inflammation and angiogenesis were decreased after TSP1-derived peptide treatment indicating a potential pathway by which TSP1 interaction with neutrophils induces CTGF in RA affected tissues.

Cited by 33 publications

References 37 publications

Immune Complexome Analysis of Serum and Its Application in Screening for Immune Complex Antigens in Rheumatoid Arthritis

Immune Complexome Analysis of Serum and Its Application in Screening for Immune Complex Antigens in Rheumatoid Arthritis

Angiogenesis and vasculogenesis in rheumatoid arthritis

Application of human umbilical cord blood-derived mesenchymal stem cells in disease models

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