Amelioration of mercuric chloride-induced physiologic and histopathologic alterations in rats using vitamin E and zinc chloride supplement

Shalan, M.G.

doi:10.1016/j.heliyon.2022.e12036

Cited by 11 publications

(9 citation statements)

References 91 publications

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“…for 28 consecutive days induced extensive nephrotoxicity (Figure ) characterized by significant changes such as epithelial necrosis and degeneration, moderate to severe interstitial congestion, and inflammatory cell infiltration. Many studies have reported that mercuric chloride intoxication in rats alters the morphology of glomerulus and renal tubules. ,,, …”

Section: Resultsmentioning

confidence: 99%

“…Many studies have reported that mercuric chloride intoxication in rats alters the morphology of glomerulus and renal tubules. 67,74,79,80 In the animals treated with ECP at the dose of 600 mg/kg b.w. and mild HgCl 2 , focal degeneration and damaged epithelial cells of the renal tubules were observed in the kidneys.…”

Section: Effect Of Ecp On Kidney Histopathology Based On Periodic Aci...mentioning

confidence: 99%

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Mercuric Chloride Induced Nephrotoxicity: Ameliorative Effect of Carica papaya Leaves Confirmed by Histopathology, Immunohistochemistry, and Gene Expression Studies

Maria Francis,

Karunakaran,

Ashfaq

et al. 2023

ACS Omega

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The present study analyzes the efficacy of the ethanolic extract of C. papaya leaves (ECP) against HgCl 2 -induced nephrotoxicity. The effects on the biochemical and percentage of body and organ weight against HgCl 2 -induced nephrotoxicity in female Wistar rats were studied. Wistar rats were divided into five groups with six animals in each group: control, HgCl 2 (2.5 mg/kg b.w.), N-acetylcysteine (NAC 180 mg/kg) + HgCl 2 , ECP (300 mg/kg b.w.) + HgCl 2 , and ECP (600 mg/kg) + HgCl 2 groups. After 28 days of study, animals were sacrificed on the 29th day to harvest the blood and kidneys for further analysis. The effect ECP was analyzed by immunohistochemistry (NGAL) and real-time PCR (KIM-1 and NGAL mRNA) in HgCl 2 -induced nephrotoxicity. The results revealed that the HgCl 2 group showed prominent damage in the proximal tubules and glomerulus of nephrons and enormous expression of NGAL in immunohistochemistry and KIM-1 and NGAL in real-time PCR compared to the control group. The simultaneous pretreatment with NAC (180 mg/kg) and ECP (600 and 300 mg/kg) reduced renal damage and expression of NGAL in immunohistochemistry and KIM-1 and NGAL gene in real-time PCR. This study attests to the nephroprotective effect of ECP against HgCl 2 -induced toxicity.

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Section: Resultsmentioning

confidence: 99%

Section: Effect Of Ecp On Kidney Histopathology Based On Periodic Aci...mentioning

confidence: 99%

Mercuric Chloride Induced Nephrotoxicity: Ameliorative Effect of Carica papaya Leaves Confirmed by Histopathology, Immunohistochemistry, and Gene Expression Studies

Maria Francis,

Karunakaran,

Ashfaq

et al. 2023

ACS Omega

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“…50 In his study, Shalan reported that tubular degeneration, necrosis and hyaline cast formation were gradually exacerbated by mercury chloride exposure. 48 In addition, Francis et al 51 demonstrated the presence of histopathologic changes such as degeneration, necrosis, interstitial congestion and inflammatory cell infiltration in mercury chloride-induced nephrotoxicity. Similarly, our results revealed congestion and nephritis in glomeruli, degeneration, vacuolization and necrosis in tubule epithelium as well as hyaline cast formation in tubule lumen and mononuclear cell infiltration in the interstitial area with mercury chloride exposure (Table 5, Figure 2).…”

Section: Discussionmentioning

confidence: 99%

“…In another study, it was reported that mercury chloride exposure caused cell damage in the liver and kidney, leading to apoptotic DNA fragmentation. 48 Caglayan et al 55 reported that Bax and p53 levels, terminal deoxynucleotidyl transferase dUNT nick end labeling (TUNEL) and caspase-3 expression increased and the apoptotic pathway was activated in mercury chloride-induced nephrotoxicity. Similarly, our results revealed that mercury chloride exposure caused an increase in caspase-3 immunoreactivity in the liver and kidney (Table 6, Figures 3 and 4).…”

Section: Discussionmentioning

confidence: 99%

“…In a study reporting that mercury chloride leads to oxidative stress and consequently liver damage by triggering an increase in hydrogen peroxide formation, intensification of lipid peroxidation and glutathione depletion, it was shown that mercury chloride exposure triggered histopathologic changes such as congestion and portal area enlargement with perivascular leukocyte infiltration in the beginning, followed by intense fibrosis, degeneration, and necrosis in hepatocytes 48 . In addition, in another study, loss of liver morphology, interstitial edema and necrosis in hepatocytes were observed with mercury chloride exposure 49 .…”

Section: Discussionmentioning

confidence: 99%

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Effects of naringenin on oxidative damage and apoptosis in liver and kidney in rats subjected to chronic mercury chloride

Kahramanoğullari,

Erişir,

Yaman

et al. 2024

Environmental Toxicology

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Mercury chloride is a type of heavy metal that causes the formation of free radicals, causing hepatotoxicity, nephrotoxicity and apoptosis. In this study, the effects of naringenin on oxidative stress and apoptosis in the liver and kidney of rats exposed to mercury chloride were investigated. In the study, 41 2‐month‐old male Wistar‐Albino rats were divided into five groups. Accordingly, group 1 was set as control group, group 2 as naringenin‐100, group 3 as mercury chloride, group 4 as mercury chloride + naringenin‐50, and group 5 as mercury chloride + naringenin‐100. For the interventions, 1 mL/kg saline was administered to the control, 0.4 mg/kg/day mercury (II) chloride to the mercury chloride groups by i.p., and 50 and 100 mg/kg/day naringenin prepared in corn oil to the naringenin groups by gavage. All the interventions lasted for 20 days. Mercury chloride administration was initiated 1 h following the administration of naringenin. When mercury chloride and the control group were compared, a significant increase in plasma urea, liver and kidney malondialdehyde (MDA) levels, in kidney superoxide dismutase (SOD), glutathione peroxidase (GSH‐Px), glutathione‐S‐transferase (GST) activities (p < .001), and a significant decrease in liver and kidney glutathione (GSH) levels (p < .001), in liver catalase (CAT) activity (p < .01) were observed. In addition, histopathological changes and a significant increase in caspase‐3 levels were detected (p < .05). When mercury chloride and treatment groups were compared, the administration of naringenin caused a decrease aspartate transaminase (AST), alanine transaminase (ALT), lactate dehydrogenase (LDH) (p < .01), urea, creatinine levels (p < .001) in plasma, MDA levels in liver and kidney, SOD, GSH‐Px, GST activities in kidney (p < .001), and increased GSH levels in liver and kidney. The addition of naringenin‐100 increased GSH levels above the control (p < .001). The administration of naringenin was also decreased histopathological changes and caspase‐3 levels (p < .05). Accordingly, it was determined that naringenin is protective and therapeutic against mercury chloride‐induced oxidative damage and apoptosis in the liver and kidney, and 100 mg/kg naringenin is more effective in preventing histopathological changes and apoptosis.

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Leek (Allium ampeloprasum var. kurrat) aqueous extract loaded on selenium nanoparticles protects against testis and brain injury induced by mercuric chloride in rats

Mumtaz,

Farag,

Farahat

et al. 2024

J Sci Food Agric

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BACKGROUNDMercuric chloride (HgCl2) is poisonous to humans and animals and typically damages the nervous system and other organs. Mercuric chloride exposition disclosed to initiation of oxidative stress pathway can result in a defect in male fertility and testis tissue. Synthesized selenium nanoparticles (SeNPs) were characterized with a diameter range minimal than 100 nm, having the effective sets of the biological matter. The present study aimed to evaluate the effect of biosynthesized SeNPs, prepared by leek extract on Wistar rats' testicles and brain.METHODSThirty‐five Wistar male rats (120–150 g) were randomly split into five groups (n = 7), orally ingested with leek aqueous extract loaded on SeNPs, and then the animals were administered with mercury II chloride (HgCl2) to induce testis injury and damage the nervous system.RESULTSThe used dose of mercuric chloride led to oxidative stress damage in the testis of the rats which was evidenced by a decrease in testosterone, luteinizing hormone (LH), follicle‐stimulating hormone (FSH) and proliferating cell nuclear antigen (PCNA) levels, and an increase in nuclear factor‐kappa B (NF‐κB) and caspase‐3. Also, HgCl2 decreased the levels of dopamine (DA), serotonin (5‐HT), norepinephrine (NE) and brain‐derived neurotrophic factor (BDNF) in the brains of rats. In addition, A decrease was observed in the levels of antioxidant markers, B‐cell lymphoma‐2 (Bcl‐2), as well as an increase in malondialdehyde (MDA), nitric oxide (NO), NF‐κB, tumor necrosis factor (TNF)‐α, interleukin (IL)‐1β and Bax in both testes and brains. Pre‐treatment with leek extract loaded on SeNPs significantly ameliorated testosterone, LH, FSH, PCNA and caspase‐3 levels in the testis and DA, 5‐HT, NE and BDNF in brains. Although the contents of MDA, NO, TNF‐α, IL‐1β, NF‐κB and Bax decreased significantly in both. glutathione, glutathione peroxidase, glutathione reductase, catalase, superoxide dismutase and Bcl‐2 levels were significantly improved in both organs.CONCLUSIONOur findings suggest that treatment with aqueous leek extract loaded on SeNPs may offer promising prospects for the advancement of anti‐inflammation activity against testis injury and also have a very key role in neurobehavioral alterations as a result of mercury toxicity. © 2024 Society of Chemical Industry.

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Amelioration of mercuric chloride-induced physiologic and histopathologic alterations in rats using vitamin E and zinc chloride supplement

Cited by 11 publications

References 91 publications

Mercuric Chloride Induced Nephrotoxicity: Ameliorative Effect of Carica papaya Leaves Confirmed by Histopathology, Immunohistochemistry, and Gene Expression Studies

Mercuric Chloride Induced Nephrotoxicity: Ameliorative Effect of Carica papaya Leaves Confirmed by Histopathology, Immunohistochemistry, and Gene Expression Studies

Effects of naringenin on oxidative damage and apoptosis in liver and kidney in rats subjected to chronic mercury chloride

Leek (Allium ampeloprasum var. kurrat) aqueous extract loaded on selenium nanoparticles protects against testis and brain injury induced by mercuric chloride in rats

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