Amelioration of N,N′-dimethylhydrazine induced colon toxicity by epigallocatechin gallate in Wistar rats

Abstract

Colon cancer is a life-threatening disease all over the world and is linked to constant oxidative stress and inflammation. Epigallocatechin gallate (EGCG), is a naturally occurring flavone possessing health benefiting pharmacological properties including antioxidant, anti-inflammatory and free radical scavenging properties. Our study investigates the role of EGCG on N,N′-dimethylhydrazine (DMH), a toxic environmental pollutant, induced colon toxicity. To investigate the effect of EGCG, Wistar rats were given EGCG for 7 days at the two doses of 10 and 20 mg/kg body weight and DMH was injected on the seventh day in all the group rats except the control. Our results indicate that DMH administration increased the oxidative stress (MDA) and depleted the glutathione and antioxidant enzyme activities (SOD, CAT, GR, GST and GPx) which was significantly ameliorated by EGCG treatment. Additionally DMH treatment upregulated inflammatory markers expression (NF-κB, COX-2 and IL-6) and enhanced mucosal damage in the colon. EGCG treatment significantly reduced inflammation and restored the normal histoarchitecture of the colon. We can conclude from the present study findings that EGCG protects the colon from DMH toxicity through its antioxidant and anti-inflammatory potential.

The effects of ellagic acid and other pomegranate (Punica granatum L.) derivatives on human gastric cancer AGS cells

“…Studies have also reported that in general, cancer and inflammation have reciprocal effects on each other. 31,58 In this regard, the anti-inflammatory effects of different parts of pomegranate fruit and its derivatives have been proven. 59,60 Thus, in this study, for the first time the effects of PFJ, PPE, and EA were investigated on altering the expression of some important inflammatory genes ( NF-κB, iNOS, IL-8, TNF-α ) in gastric cancer cells.…”

Epigallocatechin‐3‐gallate (EGCG) attenuates inflammatory responses and oxidative stress in lipopolysaccharide (LPS)‐induced endometritis via silent information regulator transcript‐1 (SIRT1)/nucleotide oligomerization domain (NOD)‐like receptor pyrin domain‐containing 3 (NLRP3) pathway

“…[ 24,25 ] The control group received same volume saline. The EGCG group was administered with EGCG (20 mg/kg body weight per day) [ 26 ] dissolved in physiological saline by intraperitoneal injection. The EGCG + LPS group was administered with EGCG (20 mg/kg body weight per day) dissolved in physiological saline by intraperitoneal injection and then administered with 50 μl of LPS (1 mg/ml) by intrauterine perfusion.…”

Inhibition and potential treatment of colorectal cancer by natural compounds via various signaling pathways

“…EGCG can enhance the sensitivity of CRC cells to 5-FU chemotherapy through the NF-κB/miR-155-5p/MDR1 axis ( 13 ). According to previous animal models, EGCG inhibits N,N’-dimethylhydrazine-induced colon tumor development through its antioxidant and anti-inflammatory potential ( 10 , 14 ). In addition, EGCG can be used in combination with sodium butyrate to induce CRC cell cycle arrest and DNA damage ( 15 ).…”