Amelioration of oxidative stress by trans-Anethole via modulating phase I and phase II enzymes against hepatic damage induced by CCl4 in male Wistar rats

Abstract

The current study was designed to assess the in vivo hepatoprotective properties of trans-Anethole and it is a principal aromatic component of star anise possessed several therapeutic properties. Results showed that CCl 4 treatment elevated the levels of different serum markers like serum glutamate oxaloacetate transaminase (SGOT) by 4.74 fold, serum glutamate pyruvate transaminase (SGPT) by 3.47 fold, aspartate alkaline phosphatase (ALP) by 3.55 fold, direct bilirubin by 3.48 fold and total bilirubin by 2.38 fold in contrast with control. It was observed that decreased levels of various liver antioxidant enzymes viz. CAT, GR, and GSH were signi cantly ameliorated by the pre-administration of rats with different doses (40, 80, and 160 mg kg -1 bw) of trans-Anethole. Furthermore, pre-treatment of trans-Anethole reduced phase I enzymes level, whereas elevated level of phase II detoxifying enzymes. Furthermore, histopathological examination showed that the treatment with trans-Anethole was potent in defending the liver from CCl 4 toxic injury and restored normal hepatic architecture. Also, trans-Anethole regulated p53 and Cyclin D1 expressions in liver tissue relative to group II treated with CCl 4 . Collectively, the ndings of the study showed a strong e ciency of trans-Anethole in ameliorating the effects caused by CCl 4 through modulation of antioxidants and xenobiotic-metabolizing enzymes.

Lycopene Alleviates Chronic Stress-Induced Liver Injury by Inhibiting Oxidative Stress-Mediated Endoplasmic Reticulum Stress Pathway Apoptosis in Rats

“…25 The range of serum ALT is approximately 26.45−63.93 U/L, and the range of serum AST is approximately 62.83−153.23 U/L in Wistar rats. 26 The results of serum AST and ALT levels of the rats in the CRS group were higher than the normal range. The H&E staining indicated that the liver histological structure of CRS rats was impaired, and the hepatic lobule structure was unclear.…”

“…Traditional markers AST and ALT are commonly used to detect liver diseases . The range of serum ALT is approximately 26.45–63.93 U/L, and the range of serum AST is approximately 62.83–153.23 U/L in Wistar rats . The results of serum AST and ALT levels of the rats in the CRS group were higher than the normal range.…”

Effects of in ovo feeding of chlorogenic acid on antioxidant capacity of postnatal broilers

“…Total protein mainly consists of albumin and globulin, and their ratio indicates protein metabolism and immune function ( Stohl et al, 2019 ; Yoshino et al, 2019 ). Blood ALT, AST, and ALP are usually used to evaluate the degree of liver oxidative damage, and their decreased activity is indicative of an improved redox status ( Dalia et al, 2017 ; Pandit et al, 2022 ). LDH and CK mainly exist in cardiomyocytes and can be released when cardiomyocytes are damaged.…”

Effects of dietary oligosaccharides on serum biochemical index, intestinal morphology, and antioxidant status in broilers

“…Temperature was not controlled during the last 24 days, and very rare amount of extreme cold or hot weather during April could be stress for broilers as heat stress resulted in decrease in ADG, ADFI, and FGR (Chang et al, 2020; Zhang et al, 2017). Serum AST and ALT are commonly used as indexes to evaluate the damage degree of liver (Pandit et al, 2021). Compared with control group, heat stress reduced liver weight and increased serum AST levels of broiler (Chen et al, 2020).…”