Amelioration of Radiation-induced Hematological and Biochemical Alterations by Alstonia scholaris (a Medicinal Plant) Extract

Abstract

The radioprotective efficacy of a hydro-alcoholic extracted material from the bark of Alstonia scholaris (ASE) was studied in mice against radiation-induced hematological and biochemical alterations. Swiss albino mice were administered ASE (100 mg/kg body weight/d for 5 consecutive day) orally prior to whole-body gamma irradiation (7.5 Gy). Radiation exposure resulted in a significant decline ( P < .001) in erythrocytes and hemoglobin until the third day, following a gradual recovery (ie, day 7), but these values did not reach normal values during the remainder of the animals' life span. Hematocrit percentage declined significantly ( P < .001) until day 15. In contrast, ASE-pretreated irradiated animals had significantly higher erythrocyte, hematocrit, and hemoglobin values than the irradiated controls. Furthermore, a significant elevation in lipid peroxidation level over normal was recorded in gamma-irradiated mice, whereas this increase was considerably lower in ASE-pretreated animals. Pretreatment with ASE caused a significant increase in glutathione levels in serum as well as in liver in comparison to irradiated animals. This study showed that ASE protects against radiation-induced hematological and biochemical alterations in Swiss albino mice.

Aloe vera modulates X-ray induced hematological and splenic tissue damage in mice

“…officinalis extract administration mitigated hematological alterations induced by ionizing radiations in mice. 9,75 Ionizing radiation induces enhanced ROS production, which leads to upregulation of inflammatory reaction as indicated by the production of proinflammatory cytokines such as IL-6 and TNF-a. 76,77 Inflammatory mediators like cytokines and chemokines result in the activation and recruitment of leucocytes to tissue from the blood circulation.…”

“…Similarly, E. officinalis linn, A. scholaris, and A. vera extracts showed less severe radiolesions and an early onset of recovery in mice blood. 8,75 LPO is a well-established mechanism involved in the oxidative deterioration of the cell architecture which may eventually lead to cell death. 80 Radiation exposure caused elevated levels of LPO in blood, liver, kidney, testes, and spleen of animals.…”

Alstonia scholaris in the ethnomedicinal and religious tradition of Coastal Karnataka, India

“…Extracts of stem bark provided protection against radiation-induced hematological and biochemical damages in mice. Gupta (2008) Hypertension Clinical study on 30 patients with bark decoction reduced elevated diastolic blood pressure Bhogayata et al (2009) Wound healing Ethanol and aqueous extracts promoted wound healing in excision, incision and dead space wound models.…”

Alstonia scholaris: It′s Phytochemistry and pharmacology

“…[3,4(pp1308-1315),5(pp337-346) -8] In Sanskrit, the plant is referred to as phalagaruda, sapthaparna, and saptaparni (sapta means 7 and parna or parni means leaves) because the leaves are found in whorls of 7 [ Figure 1]. Hydro-alcoholic extract of bark Male Swiss albino mice were studied for radiation-induced hematological and biochemical alterations [62] Dose tolerance of Alstonia scholaris extract (ASE), Dose reduction factor (DRF), Endogenous colonies in spleen, LPx and GSH were studied in Swiss albino mice [63] A. scholaris extract (ASE) of bark Human lung cancer cell lines, MOR-P (adenocarcinoma) and COR-L23 (large cell carcinoma), using the SRB assay [73] Alteration in phosphatase activity Bark extract Exposed to gamma radiation at the source to surface distance (SSD) of 77.5 cm to deliver the dose rate of 1.32 Gy/min [74] Chemomodulatory activity A. scholaris extract (ASE) was studied in combination with berberine hydrochloride (BCL) EAC bearing mice [75] Combination of 120 mg/kg of ASE with 25 mg/kg of CPA was most effective…”