Amelioration of selenite toxicity and cataractogenesis in cultured rat lenses by Vitex negundo

Rooban, B.N.; Sasikala, V.; Sahasranamam, V.; Abraham, Annie

doi:10.1007/s00417-010-1598-0

Cited by 16 publications

(7 citation statements)

References 30 publications

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“…Calcium concentrations in the lens samples were analyzed flame photometrically with standard prepared from CaCO 3 and deionized water. The results were expressed as μmoles/g wet tissue (21).…”

Section: Methodsmentioning

confidence: 99%

Bioactive Derivatives of Curcumin Attenuate Cataract Formation In Vitro

et al. 2012

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In this study, curcumin derivatives salicylidenecurcumin (CD1) and benzalidenecurcumin (CD2)] were prepared, and their biological activity was compared in in vitro selenite-induced cataract model. The antioxidant activity was studied using DPPH radical scavenging assay. Knoevenagel condensates of curcumin exhibited higher DPPH radical scavenging activity compared with curcumin. The anticataractogenic potential of curcumin derivatives was analyzed using lens organ culture method. The activity of antioxidant enzymes and calcium homeostasis was reversed to near normal levels following treatment in organ cultured rat lenses. These results indicated that curcumin and its derivatives-CD1 and CD2-are beneficial against selenite-induced cataract in vitro. Of these, CD1 is having higher bioactive potential compared with curcumin and CD2. Blindness is a common condition globally, affecting approximately 45 million people, and more than a third of blindness is caused by cataract (1). Approximately 25% of the population with age over 65 and about 50% over 80 have serious loss of vision because of cataract (2). Cataract is a protein aggregation disease affecting vision in humans. At present, the most effective treatment for cataract is surgical extirpation of the opaque lens, but it is expensive and not free from risk factors (3). It is widely accepted that oxidative stress is a significant factor in the genesis of cataract (4). Oxidative stress may result from an imbalance between the production of reactive oxygen species (ROS) and the cellular antioxidant defense mechanisms.Oxidative metabolism is clearly important in maintaining the lens in a transparent state. The lens is metabolically active and requires nourishment to maintain its growth and transparency. Like other organs, lens has a well-designed defense system against oxidation. It uses both enzymatic and non-enzymatic defense system to neutralize free radicals that are clearly involved in the pathophysiological changes in the eye (5). Selenite-overdose cataract is an extremely rapid and convenient model to study experimental cataract. Major events of selenite cataract are loss of calcium homeostasis, reactive oxygen species generation, and decreased water soluble proteins (6).Medicinal plants often contain highly active pharmacological compounds. Many of these compounds, isolated from a part or whole of the medicinal herbs, act as pharmaceutical drugs having therapeutic potential against cataract (7-11). Curcumin is well known for its flavoring and medical properties and shown to exhibit antioxidant, antiinflammatory, antimicrobial, and anticarcinogenic activities. The medicinal properties of curcumin are attributed to its radical scavenger activity and are shown to scavenge free radicals such as hydroxyl radicals, superoxide radicals, singlet oxygen, peroxyl radicals, and peroxynitrite (12). Thus, curcumin can reduce the oxidative stress in tissues and hence a potential candidate as an anticataract agent. In spite of its efficacy and safety, curcumin has not yet b...

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Section: Methodsmentioning

confidence: 99%

Bioactive Derivatives of Curcumin Attenuate Cataract Formation In Vitro

et al. 2012

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“…The glucoside of isorhamnetin (methylated quercetin), isolated as a bioactive flavonoid from the leaves of Cochlospermum religiosum (Gayathri et al ., 2010 ) and flavonoid fraction isolated from fresh leaves of Vitex negundo (Rooban et al ., 2011 ) protected enucleated rat eye lenses against selenite-induced cataract in an in vitro culture model.…”

Section: Anticataract Action Of Flavonoidsmentioning

confidence: 99%

Natural flavonoids as potential multifunctional agents in prevention of diabetic cataract

Štefek¹

2011

Interdisciplinary Toxicology

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Cataract is one of the earliest secondary complications of diabetes mellitus. The lens is a closed system with limited capability to repair or regenerate itself. Current evidence supports the view that cataractogenesis is a multifactorial process. Mechanisms related to glucose toxicity, namely oxidative stress, processes of non-enzymatic glycation and enhanced polyol pathway significantly contribute to the development of eye lens opacity under conditions of diabetes. There is an urgent need for inexpensive, non-surgical approaches to the treatment of cataract. Recently, considerable attention has been devoted to the search for phytochemical therapeutics. Several pharmacological actions of natural flavonoids may operate in the prevention of cataract since flavonoids are capable of affecting multiple mechanisms or etiological factors responsible for the development of diabetic cataract. In the present paper, natural flavonoids are reviewed as potential agents that could reduce the risk of cataract formation via affecting multiple pathways pertinent to eye lens opacification. In addition, the bioavailability of flavonoids for the lens is considered.

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“…Ocimum sanctum (Lamiaceae) Extract of plant inhibition of polyol accumulation (Halder et al, 2003) Origanum vulgare Extract of plant Inhibit the oxidative damage and reduces the lipid peroxidation. (Dailami et al, 2010) Pterocarpus marsupium (Fabaceae) aqueous extract Inhibits the diabetes induced cataract (Vats et al, 2004) Silybum marianum (Compositae) seeds extract Inhibit the oxidative damage (Huseini et al,2004) Trigonella foenum graceum (Fabaceae) Seed extract Inhibit the generated oxidative stress (Lija et al, 2006) Vitex negundo (verbanaceae) flavonoids fraction Inhibits the oxidative stress (Rooban et al, 2011) Withania somnifera (solanaceae) Extract of plant Inhibits the oxidative stress (Thiagarajan et al, 2003) PLANT PRODUCTS AGAINST CATARACTS reactive oxygen species, which causes the lipid peroxidations and thus play major role in development of diabetic secondary complications in kidney, blood vessels, eye and nerve . The natural antioxidant such as ascorbic acid, vitamin E, and carotinoids have been screened and it was found that antioxidant has protective action against cataract.…”

Section: Plant Products Against Cataractsmentioning

confidence: 99%

Cataract: A major secondary diabetic complication

Patel¹,

Jivani

Malaviya³

et al. 2012

Int Curr Pharm J

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Cataract is a visual impairment occurs due to the opacification of crystalline lens. It affects around 17 billion peoples worldwide, although incidence of cataracts is increasing day by day among the elderly persons. Still today except surgery no other effective treatment have been successfully developed so far, thus this present review is focused to highlights the etiological aspects, risk factors along with possible pharmacological prevention and animal models which are widely used for screening anticataract activity. The present review includes the list of plants and their phytoconstituents which have been evaluated pharmacologically for the treatment of cataract. From the review it can be concluded that antioxidant properties of plants phytochemicals are responsible for the anticataract activity.DOI: http://dx.doi.org/10.3329/icpj.v1i7.10813International Current Pharmaceutical Journal 2012, 1(7): 180-185

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Amelioration of selenite toxicity and cataractogenesis in cultured rat lenses by Vitex negundo

Abstract: These results suggest that FVN treatment prevented selenite toxicity and cataractogenesis by maintaining antioxidant status, calcium homeostasis, protecting sulfhydryl group, and decreasing oxidative stress in lens, which may be due to its protective effects.

Cited by 16 publications

References 30 publications

Bioactive Derivatives of Curcumin Attenuate Cataract Formation In Vitro

Bioactive Derivatives of Curcumin Attenuate Cataract Formation In Vitro

Natural flavonoids as potential multifunctional agents in prevention of diabetic cataract

Cataract: A major secondary diabetic complication

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