Amelioration of streptozotocin-induced diabetic nephropathy by melatonin, quercetin, and resveratrol in rats

Abstract

<jats:p> The role of oxygen radicals are known for the pathogenesis of kidney damage. The aim of the present study was to investigate the antioxidative effects of melatonin, quercetin, and resveratrol on streptozotocin (STZ)-induced diabetic nephropathy in rats. A total of 35 male Wistar rats were divided into 5 groups as follows: control, diabetes mellitus (DM), DM + melatonin, DM + quercetin, and DM + resveratrol. All the injections started on the same day of single-dose STZ injection and continued for 30 days. At the end of this period, kidneys were removed and processed for routine histological procedures. Biochemical parameters and morphological changes were examined. In DM group, blood glucose levels were significantly increased, whereas body weights were decreased compared with the control group. Significant increases in blood urea nitrogen and tissue malondialdehyde (MDA) levels and decreases in superoxide dismutase and catalase activities were detected in DM group. Administration of melatonin, quercetin, and resveratrol significantly reduced these values. Melatonin was more efficient in reducing MDA levels than other antioxidants ( p &lt; 0.05). STZ-induced histopathological alterations including epithelial desquamation, swelling, intracytoplasmic vacuolization, brush border loss and peritubular infiltration. Additionally, basement membrane thickening and sclerotic changes were observed in glomerulus. Transforming growth factor-β1 positive cells were also increased. Melatonin, quercetin, and resveratrol significantly reduced these histopathological changes. Our results indicate that melatonin, quercetin, and resveratrol might be helpful in reducing diabetes-induced renal damage </jats:p>

DNA protective and antioxidative effects of melatonin in streptozotocin-induced diabetic rats

“…In the present study, an i.p. administration of MEL at 10 mg/kg was incapable of stopping the body weight loss in diabetic rats (P > 0.05) and this finding supports previous research studies (Cam et al, 2003;Sudnikovich et al, 2007;Oršolić et al, 2011;Elbe et al, 2015;Gleissner, 2015). MEL is capable of initiating weight loss in adult rats and plays an extra role in the regulation of body weight through the stimulation of brown adipose tissue metabolism (Korkmaz et al, 2012).…”

“…Examined blood glucose levels were not significantly changed at the end of the treatment among the untreated diabetic rats and the MEL-treated diabetic rats. This finding is in agreement with previous research studies ( Andersson and Sandler, 2001;Vural et al, 2001;Aksoy et al, 2003), although the blood glucose levels of the present study were different from those of some published studies (Bibak et al, 2014;Elbe et al, 2015). Gorgun et al (2002) reported that the administration of MEL prior to or after STZ treatment decreases plasma glucose and HbA 1c levels.…”

“…MDA levels were significantly higher in all tissues of the diabetic rats, but MEL administration reduced the MDA levels in the liver, kidney, and brain tissues, except for the pancreas tissues. Comparable results were also obtained by some researchers (Vural et al 2001;Aksoy et al, 2003;Eşrefoğlu et al 2014;Elbe et al, 2015).…”

Sirtuin 1: A Target for Kidney Diseases

“…Consistent with its dual cellular localization, SIRT1 targets can be found in both the nucleus and the cytoplasm. SIRT1 activation exerts protective effects on multiple organs upon oxidative stress, including kidney (12,(20)(21)(22)(23), whereas SIRT1 knockout (KO) mice show aggravation of renal changes occurring in diabetes and acute kidney injury (12,24).…”

The effects of caffeic acid phenethyl ester on streptozotocin-induced diabetic liver injury

“…The STZ-induced diabetic rat is one of the animal models of human diabetes mellitus (19). STZ is given as a single injection of 40-60 mg/kg to provoke diabetes in rats (20)(21)(22). We used STZ, an antibiotic produced by Streptomyces achromogenes, at a single dose of 55 mg/kg.…”