Ameliorative effect of fractionated low-dose gamma radiation in combination with ellagic acid on nicotine-induced hormonal changes and testicular toxicity in rats

Ashoub, Aliaa H.; Abdel‐Naby, Doaa H.; Safar, Marwa M.; El‐Ghazaly, Mona A.; Kenawy, Sanaa A.

doi:10.1007/s11356-020-12334-2

Cited by 6 publications

(13 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Legendre et al (2016) also identified that low‐dose depleted uranium could affect the production of these sex steroid hormone levels in adult rats. Interestingly, we did not detect acute physiological damages/changes in the male reproductive organ, including testis and epididymis, consistent with the latest finding that chronic LDR exposure associated with the Fukushima plant accident did not affect the characteristics and functions of male raccoons' reproductive system (Ashoub et al, 2021; Komatsu et al, 2020). Nevertheless, the current study found that LDR indeed increased the content of testosterone without the remarkable detectable damages in radiosensitive organs.…”

Section: Discussionsupporting

confidence: 90%

Low‐dose radiation‐induced demethylation of 3β‐HSD participated in the regulation of testosterone content

Huang

Qiu

Yue³

et al. 2021

J of Applied Toxicology

View full text Add to dashboard Cite

The effects of low‐dose radiation (LDR, ≤0.1 Gy) on living organisms have been the hot areas of radiation biology but do not reach a definitive conclusion yet. So far, few studies have adequately accounted for the male reproductive system responses to LDR, particularly the regulation of testosterone content. Hence, this study was designed to evaluate the effects of LDR on Leydig cells and testicular tissue, especially the ability to synthesize testosterone. We found that less than 0.2‐Gy 60Co gamma rays did not cause significant changes in the hemogram index and the body weight; also, pathological examination did not find obvious structural alterations in testis, epididymis, and other radiation‐sensitive organs. Consistently, the results from in vitro showed that only more than 0.5‐Gy gamma rays could induce remarkable DNA damage, cycle arrest, and apoptosis. Notably, LDR disturbed the contents of testosterone in mice serums and culture supernatants of TM3 cells and dose dependently increased the expression of 3β‐HSD. After cotreatment with trilostane (Tril), the inhibitor of 3β‐HSD, increased testosterone could be partially reversed. Besides, DNA damage repair‐related enzymes, including DNMT1, DNMT3B, and Sirt1, were increased in irradiated TM3 cells, accompanying by evident demethylation in the gene body of 3β‐HSD. In conclusion, our results strongly suggest that LDR could induce obvious perturbation in the synthesis of testosterone without causing organic damage, during which DNA demethylation modification of 3β‐HSD might play a crucial role and would be a potential target to prevent LDR‐induced male reproductive damage.

show abstract

Section: Discussionsupporting

confidence: 90%

Low‐dose radiation‐induced demethylation of 3β‐HSD participated in the regulation of testosterone content

Huang

Qiu

Yue³

et al. 2021

J of Applied Toxicology

View full text Add to dashboard Cite

show abstract

“… 7 , 36 , 37 Also, low doses intensified the antioxidant, antiapoptotic, and anti-inflammatory effects of exogenous antioxidants administered to rats against liver and testis injuries induced by different toxicants. 13 , 38 , 39 As well, the clinical trial recorded by Yu et al showed that low dose radiation-mediated radioadaptive response can alleviate high dose radiation toxicities, improve the short-term efficacy of radiotherapy, and improve the survival of the lung cancer patients. 40 …”

Section: Discussionmentioning

confidence: 99%

Low-Dose Gamma Radiation Modulates Liver and Testis Tissues Response to Acute Whole Body Irradiation

2022

View full text Add to dashboard Cite

Aim This work aims to investigate whether the pre-exposure to low dose/low dose rate (40 mGy, 2.2 mGy/hour) γ-radiation as a priming dose can produce a protective effect against the subsequent high one (4 Gy, .425 Gy/minute). Methods Rats were divided into Group I (control), Group II (L); exposed to 40 mGy, Group III (H); exposed to 4 Gy, and Group IV (L+H); exposed to 40 mGy 24 hours before the exposure to 4Gy. The molecular and biochemical changes related to oxidative stress, DNA damage, apoptosis, and mitochondrial activity in the liver and testis were studied 4 hours after irradiation. Results Exposure to 40 mGy before 4 Gy induced a significant increase in the levels of Nrf2, Nrf2 mRNA, TAC, and mitochondrial complexes I & II accompanied by a significant decrease in the levels of LPO, 8-OHdG, DNA fragmentation, TNF-α, caspase-3, and caspase-3 mRNA compared with H group. Conclusion Exposure to low-dose γ-radiation before a high dose provides protective mechanisms that allow the body to survive better after exposure to a subsequent high one via reducing the oxidative stress, DNA damage, and apoptosis-induced early after irradiation. However, further studies are required to identify the long-term effects of this low dose.

show abstract

“…Non-anesthetized rats were placed in the plastic sample tray and irradiated at 0.5 Gray (Gy) applied as a fractionated exposure (2 x 0.25 Gy /1week interval) delivered at a 0.46 Gy/min rate. The selection of radiation dose and exposure period was based on previous studies by our research groups Ashoub et al (2021) and El-Ghazaly et al (2020).…”

Section: Irradiation Proceduresmentioning

confidence: 99%

“…The selection of nicotine dose and duration of administration was according to Ashoub et al (2021). In addition to numerous studies, for instance, Oyeyipo et al (2010), Kolawole et al (2019), and Ukwenya et al (2020).…”

Section: Experimental Designmentioning

confidence: 99%

“…Seldom researches demonstrated the therapeutic benefits of LDR in conjunction with natural products against nicotine-induced testicular damage. Our research group Ashoub et al (2021) is concerned with studying the effect of LDR in combination with ellagic acid against nicotine-induced testicular toxicity. This prompted us to continue in the present study to investigate the effect of more natural antioxidants, for instance, EGCG and RUT as an adjunctive therapy on the therapeutic activity of LDR on the nicotine-induced testicular injury rat model.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Therapeutic Effects of Epigallocatechin-3-gallate and Rutin, either Alone or in Combination with Low-dose radiation, against Testicular Injury Evoked by Nicotine in Rats

et al. 2022

Self Cite

View full text Add to dashboard Cite

G ROWING evidence suggests that nicotine, the addictive component of cigarettes, plays a direct role in testicular injury and infertility. The present study was intended to investigate the therapeutic effect of epigallocatechin-3-gallate (EGCG) and rutin (RUT), either alone or in combination with a low-dose radiation (LDR), against testicular injury evoked by nicotine in rats. For the induction of testicular injury, nicotine (1mg/kg) was administered orally for 30 days. Following that, rats were administered EGCG (100 mg/ kg), RUT (30 mg/kg) orally, either alone or in combination with LDR (2 x 0.25 Gy) for an additional 14 days. Rats were sacrificed on day 45, the testes were then dissected for histopathological analysis, and several biochemical parameters in serum and testicular tissue were also evaluated. The results showed that nicotine administration significantly increased the testicular thiobarbituric acid reactive substances and decreased the reduced glutathione contents. Besides, the activities of testicular androgenic enzymes (3 beta-hydroxysteroid dehydrogenases and 17 beta-hydroxysteroid dehydrogenases) were reduced, whereas serum lactate dehydrogenase activity was considerably raised. In addition, the follicle-stimulating hormone, luteinizing hormone, and testosterone serum levels were reduced, indicating hormonal alterations. The testicular seminiferous tubules structure was also deformed after histological examination. On the other hand, treatment with LDR combined with either EGCG or RUT dramatically reduced the deleterious effects of nicotine compared to their individual effects, as evidenced by biochemical and histological findings. Accordingly, exposure to LDR combined with natural antioxidants, either EGCG or RUT, may be a promising candidate for treating testicular injury caused by nicotine.

show abstract

Ameliorative effect of fractionated low-dose gamma radiation in combination with ellagic acid on nicotine-induced hormonal changes and testicular toxicity in rats

Cited by 6 publications

References 66 publications

Low‐dose radiation‐induced demethylation of 3β‐HSD participated in the regulation of testosterone content

Low‐dose radiation‐induced demethylation of 3β‐HSD participated in the regulation of testosterone content

Low-Dose Gamma Radiation Modulates Liver and Testis Tissues Response to Acute Whole Body Irradiation

The Therapeutic Effects of Epigallocatechin-3-gallate and Rutin, either Alone or in Combination with Low-dose radiation, against Testicular Injury Evoked by Nicotine in Rats

Contact Info

Product

Resources

About