Ameliorative effect of kaempferol, a flavonoid, on oxidative stress in streptozotocin-induced diabetic rats

Al‐Numair, Khalid S.; Govindasamy, Chandramohan; Veeramani, Chinnadurai; Alsaif, Mohammed A.

doi:10.1179/1351000214y.0000000117

Cited by 149 publications

(108 citation statements)

References 51 publications

(44 reference statements)

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“…Increased MDA (Table ) in the liver of diabetic rats confirm oxidative damage. Fractions A (quercetin) and B (kaempferol) reversed the alterations in antioxidant enzymes (SOD and CAT), glutathione and lipid peroxidation product (MDA) suggesting antioxidant activity as demonstrated in studies in different experimental models (Aherne & O'Brien, ; Al‐Numair et al, ; Gupta, Vikram, Tripathi, Ramarao, & Jena, ; Kaindl, Eyberg, Rohr‐udilova, Heinzle, & Marian, ).…”

Section: Resultsmentioning

confidence: 81%

“…Kaempferol and quercetin have been reported to improve the lipid profile of diabetic rats (Al‐Numair, Chandramohan, Veeramani, & Alsaif, ; Yang & Kang, ). Consistent with these studies, the isolated compounds significanlty lowered elevated TC, TAG and LDLc, and raised HDLc in the serum of alloxan‐induced diabetic rats when compared to the control rats (Table ).…”

Section: Resultsmentioning

confidence: 99%

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Bioactivity-guided isolation of antidiabetic principles from the methanolic leaf extract ofBryophyllum pinnatum

et al. 2018

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The antidiabetic principles from methanolic leaf extract of Bryophyllum pinnatum were isolated. The leaf extract was fractionated on silica gel using column chromatography and identified using nuclear magnetic resonance (NMR) spectrometry. The ethylacetate fraction of the partitioned methanolic extract of B. pinnatum lowered blood glucose of alloxan‐induced diabetic rats and inhibited α‐amylase and α‐glucosidase with IC50 137.89 and 110.15 µg/mL, respectively. In addition to lowering blood glucose, fractions A and B inhibited α‐amylase with IC50 57.43 and 43.84 µg/mL and α‐glucosidase with IC50 11.15 and 25.79 µg/mL, respectively. 1H and 13C NMR showed that fractions A and B are quercetin and kaempeferol, respectively. Molecular docking revealed that kaempferol and quercetin interacted with amino acid residues that bind/hydrolyze substrate molecules These compounds reversed altered lipid profile and oxidative stress biomarkers. Our findings showed that kaempferol and quercetin are responsible for the antidiabetic activity of B. pinnatum. Practical application Bryophyllum pinnatum is an edible vegetable plant in some parts of Nigeria, and its consumption could improve diabetic condition and lower postprandial glucose. Furthermore, extract of the leaves could be developed into food supplements for managing diabetes and its associated complications including dyslipidaemia and oxidative stress.

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Section: Resultsmentioning

confidence: 81%

Section: Resultsmentioning

confidence: 99%

Bioactivity-guided isolation of antidiabetic principles from the methanolic leaf extract ofBryophyllum pinnatum

et al. 2018

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“…The present study results are in par with previous studies, where reduced MDA levels with increased SOD, CAT, and GPx activity levels were observed in KF‐treated ISO‐induced cardiac toxicity in rats (Suchal et al, ). Recently, a few other studies showed that KF treatment reduced the LPO and bought back antioxidant enzymes like SOD, CAT, GPx, and GST in liver, kidney, and heart of diabetic rats (Al‐Numair, Chandramohan, Veeramani, & Alsaif, ) and ISO‐induced myocardial injury rats (Zhou, Ren et al, ).…”

Section: Discussionmentioning

confidence: 99%

The protective effect of kaempferol on heart via the regulation of Nrf2, NF‐κβ, and PI3K/Akt/GSK‐3β signaling pathways in isoproterenol‐induced heart failure in diabetic rats

Zhang

Guo

Wang

et al. 2019

Drug Development Research

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This study was designed to delineate the effect of kaempferol (KF) on heart failure (HF) in diabetic rats. Streptozotocin‐induced male diabetic rats received KF orally at 10 and 20 mg/kg for 42 consecutive days. In last 2 days of the experimental period, isoproterenol was subcutaneously injected at 85 mg/kg to induce HF. The hearts were processed for hemodynamic, biochemical, molecular, and histological investigations. Systolic blood pressure, diastolic blood pressure, and mean arterial blood pressure were elevated in KF‐treated HF‐induced diabetic rats. Moreover, KF treatment resulted in decreased fasting blood glucose and glycosylated hemoglobin levels with increased serum insulin levels. Besides, serum cardiac injury markers like troponin‐I, creatine kinase‐muscle/brain, lactate dehydrogenase, and brain natriuretic peptide levels were significantly reduced in KF treatment. KF treatment has shown decrease in cardiac heme oxygenase‐1, nuclear factor erythroid 2–related factor 2 (Nrf‐2), and γ‐glutamylcysteine synthetase with increased Keap1 mRNA levels. The cardioprotection of KF was improved by inhibition of apoptosis via blocking phosphorylation of Akt/glycogen synthase kinase (GSK)‐3β and p38 mitogen‐activated protein‐kinase/extracellular signal‐regulated kinases signaling pathways in HF‐induced diabetic rats. Moreover, reduced cardiac apoptosis in KF treatment was confirmed by decreased terminal deoxynucleotidyl transferase dUTP nick‐end labeling (TUNEL) positive cells, histopathological changes in HF‐induced diabetic rats. Therefore, the cardioprotective effect of KF is attributed to the regulation of Nrf2, nuclear factor kappa‐light‐chain‐enhancer of activated B cells, and Akt/GSK‐3β signaling pathways in HF‐induced diabetic rats.

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“…Luteolin ameliorates insulin resistance and inhibits alpha-amylase and alpha-glucosidase enzyme activities [35, 36]. Similarly, kaempferol is another typical dietary flavonoid that was shown to have many beneficial activities including anti-diabetic, antioxidant, and anti-inflammatory through promoting functions of pancreatic β-cell [37]. It was also shown to have positive effect on lipid metabolism, stimulate insulin-dependent glucose uptake and improve insulin resistance [38].…”

Section: Discussionmentioning

confidence: 99%

Anti-Diabetic Potential of the Leaves of Anisomeles malabarica in Streptozotocin Induced Diabetic Rats

Kotha

Badri

Nagalapuram

et al. 2017

Cell Physiol Biochem

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Background/Aims: Diabetes mellitus is a pandemic metabolic disorder that is affecting a majority of populations in recent years. There is a requirement for new drugs that are safer and cheaper due to the side effects associated with the available medications. Methods: We investigated the anti-diabetic activity of leaves of Anisomeles malabarica following bioactivity guided fractionation. The different solvent (hexane, ethyl acetate, methanol and water) extracts of A. malabarica leaves were used in acute treatment studies to evaluate and identify the active fraction. The ethyl acetate extract was subjected to further fractionation using silica gel column chromatography and the compounds were identified by LC-SRM/MS and GC-MS. Additional chronic treatment studies were carried out using this active fraction (AMAF) for 30 days in experimental diabetic rats. Fasting blood glucose (FBG), glycosylated hemoglobin (HbA1c), plasma insulin levels and glucose tolerance were measured along with insulin resistance/sensitivity indicators (HOMA-IR, HOMA-β and QUICKI) to assess the beneficial effects of A. malabarica in the management of diabetes mellitus. Results: Among the different solvent extracts tested, ethyl acetate extract showed maximum (66%) anti-hyperglycemic activity. The hexane and ethyl acetate (1: 1) fraction that has maximum anti-diabetic activity was identified as active fraction of A. malabarica (AMAF). The FBG, HbA1c, plasma insulin levels and insulin sensitivity/resistance indicators such as glucose tolerance, HOMA-IR, HOMA-β and QUICKI were significantly improved to near normal in diabetic rats treated with AMAF. Further, we identified key flavonoids and fatty acids as the anti-diabetic active principles from the AMAF of A. malabarica leaves. Conclusion: The results of our study suggest that Anisomeles malabarica has potential anti-diabetic activity in STZ induced diabetic rats.

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Ameliorative effect of kaempferol, a flavonoid, on oxidative stress in streptozotocin-induced diabetic rats

Cited by 149 publications

References 51 publications

Bioactivity-guided isolation of antidiabetic principles from the methanolic leaf extract ofBryophyllum pinnatum

Bioactivity-guided isolation of antidiabetic principles from the methanolic leaf extract ofBryophyllum pinnatum

The protective effect of kaempferol on heart via the regulation of Nrf2, NF‐κβ, and PI3K/Akt/GSK‐3β signaling pathways in isoproterenol‐induced heart failure in diabetic rats

Anti-Diabetic Potential of the Leaves of Anisomeles malabarica in Streptozotocin Induced Diabetic Rats

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