Ameliorative effect of low molecular weight peptides from the head of red shrimp (Solenocera crassicornis) against cyclophosphamide-induced hepatotoxicity in mice

Jiang, Shuoqi; Zhang, Zhuangwei; Zhang, Zhuangwei; Zhang, Zhoufeng; Yang, Zuisu; Yu, Fangmiao; Ding, Guofang

doi:10.1016/j.jff.2020.104085

Cited by 27 publications

(36 citation statements)

References 47 publications

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“…In this study, SCHPs-F1 presented antioxidant activity in vitro including radical scavenging and reducing power. Interestingly, it has been reported that SCHPs-F1 ameliorated hepatotoxicity by reducing the oxidative stress status of liver in CTX-induced mice [14]. In the present study, we investigated the effect of SCHPs-F1 on CTX-induced nephrotoxicity in a mouse model.…”

Section: Discussionmentioning

confidence: 91%

“…SCHPs-F1 was prepared according to the process described in our previous report [ 14 ]. In brief, defatted shrimp heads were homogenized and allowed to react in a pH-adjusted (pH = 3.0) pepsin-containing aqueous solution (2500 U/g, m/m = 1:10) for 4 h. Then, the pH was adjusted to 8.0 under optimal trypsin conditions (2500 U/g) in a reaction system for 4 h. The solution was heated to inactivate the enzyme, and the supernatant was collected (CF16RN high-speed microcentrifuge, Himac, Tokyo, Japan), filtered through a microfilter (0.22 μm), and ultrafiltered using a 1 kDa membrane.…”

Section: Methodsmentioning

confidence: 99%

“…Several studies have been executed to prepare protein hydrolysates and bioactive peptides of varying sizes using industrial waste from various shrimp processing leftovers [11][12][13]. To further assess commercial high-value applications of Solenocera crassicornis shrimp heads, we prepared low molecular weight peptides (MW less than 1 kDa, SCHPs-F1) and found they could effectively alleviate CTX-induced hepatotoxicity in mice [14]. Based on the toxicological and metabolic mechanism of action of CTX that involves the induction of multiple organ toxicity [8], we speculated that SCHPs-F1 supplementation may also have the potential to alleviate CTX-induced nephrotoxicity.…”

Section: Introductionmentioning

confidence: 99%

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Protective Effect of Low Molecular Weight Peptides from Solenocera crassicornis Head against Cyclophosphamide-Induced Nephrotoxicity in Mice via the Keap1/Nrf2 Pathway

et al. 2020

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The major component of the Solenocera crassicornis head protein hydrolysates-fraction 1 (SCHPs-F1) are low molecular weight peptides (MW < 1 kDa). In this study, we investigated the potential renoprotective effects of SCHPs-F1 in a cyclophosphamide (CTX) toxicity mouse model. In brief, 40 male mice were randomly divided into 5 groups and received either saline or 80 mg/kg body weight (BW) CTX by intraperitoneal injection for 5 days, followed by either saline or SCHPs-F1 (100, 200, and 400 mg/kg BW) by intragastric administration for 15 days. SCHPs-F1 treatment significantly reversed the CTX-induced decreases in the levels of blood urea nitrogen (BUN), creatinine (CRE), and cytochrome P450 (CYP450), as well as the renal histological lesions. Furthermore, the results indicated that SCHPs-F1 potentially alleviated CTX-induced nephrotoxicity through mitigating inflammatory responses, oxidative stress, and apoptosis status of the kidneys, as evidenced by decreased levels of malondialdehyde (MDA), interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, and interferon (IFN)-γ and increased levels of total antioxidant capacity (T-AOC), catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). Moreover, overexpression of pro-apoptotic proteins pair B-cell lymphoma-2 (Bcl-2)-associated X (Bax)/Bcl-2, cysteinyl aspartate specific proteinase (caspase)-3 and caspase-9 in renal tissues were suppressed by treatment with SCHPs-F1. In addition, the protein levels of the antioxidant factor nuclear factor erythroid-2 related factor 2 (Nrf2) and the expression levels of its downstream target genes heme-oxygenase (HO-1), glutamate-cysteine ligase modifier subunit (GCLM) and NAD(P)H dehydrogenase (quinone) 1 (NQO-1) were stimulated by treatment with SCHPs-F1 in the CTX-induced renal injury model. Taken together, our data suggested that SCHPs-F1 could provide a novel potential strategy in mitigating the nephrotoxicity caused by CTX.

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Section: Discussionmentioning

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Protective Effect of Low Molecular Weight Peptides from Solenocera crassicornis Head against Cyclophosphamide-Induced Nephrotoxicity in Mice via the Keap1/Nrf2 Pathway

et al. 2020

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“…Cy can damage intestinal mucosa, cause intestinal damage, affect the secretion of intestinal cytokines, and regulate TLRs, NF-κB and other signaling pathways. A research study found that peptides from Solenocera crassicornis have immunomodulatory activity through the NF-κB signaling pathway, and have an important protective effect on immunosuppressed mouse models [ 68 ]. Glycosaminoglycan from Apostichopus japonicus [ 66 ] and Cordyceps sinensis [ 14 ] polysaccharides can balance the imbalance of cytokine secretion, regulate the NF-κB pathway, and repair Cy-induced intestinal immune dysfunction in mice.…”

Section: Discussionmentioning

confidence: 99%

Ameliorative Effects of Peptides Derived from Oyster (Crassostrea gigas) on Immunomodulatory Function and Gut Microbiota Structure in Cyclophosphamide-Treated Mice

et al. 2021

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The intestinal flora is recognized as a significant contributor to the immune system. In this research, the protective effects of oyster peptides on immune regulation and intestinal microbiota were investigated in mice treated with cyclophosphamide. The results showed that oyster peptides restored the indexes of thymus, spleen and liver, stimulated cytokines secretion and promoted the relative mRNA levels of Th1/Th2 cytokines (IL-2, IFN-γ, IL-4 and IL-10). The mRNA levels of Occludin, Claudin-1, ZO-1, and Mucin-2 were up-regulated, and the NF-κB signaling pathway was also activated after oyster peptides administration. Furthermore, oyster peptides treatment reduced the proportion of Firmicutes/Bacteroidetes, increased the relative abundance of Alistipes, Lactobacillus, Rikenell and the content of short-chain fatty acids, and reversed the composition of intestinal microflora similar to that of normal mice. In conclusion, oyster peptides effectively ameliorated cyclophosphamide-induced intestinal damage and modified gut microbiota structure in mice, and might be utilized as a beneficial ingredient in functional foods for immune regulation.

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“…Recently, marine bioactive peptides have attracted the attention of researchers, and many biologically active peptides with specific activities have been discovered from the ocean, all of which exhibited a wide range of biological functions, including liver protection [ 10 ]; immune regulation; and antihypertensive, antioxidant, and antibacterial activities [ 11 ]. However, reports regarding peptides with kidney-protecting activity are scarce.…”

Section: Introductionmentioning

confidence: 99%

Ameliorating Effect of Pentadecapeptide Derived from Cyclina sinensis on Cyclophosphamide-Induced Nephrotoxicity

et al. 2020

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Cyclophosphamide (CTX) is a widely used anticancer drug with severe nephrotoxicity. The pentadecapeptide (RVAPEEHPVEGRYLV) from Cyclina sinensis (SCSP) has been shown to affect immunity and to protect the liver. Hence, the purpose of this study was to investigate the ameliorating effect of SCSP on CTX-induced nephrotoxicity in mice. We injected male ICR mice with CTX (80 mg/kg·day) and measured the nephrotoxicity indices, levels of antioxidant enzymes, malondialdehyde (MDA), inflammatory factors, as well as the major proteins of the NF-κB and apoptotic pathways. Cyclophosphamide induced kidney injury; the levels of kidney-injury indicators and cytokines recovered remarkably in mice after receiving SCSP. The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) increased, while there was a significant decrease in MDA levels. The kidney tissue damage induced by CTX was also repaired to a certain extent. In addition, SCSP significantly inhibited inflammatory factors and apoptosis by regulating the NF-κB and apoptotic pathways. Our study shows that SCSP has the potential to ameliorate CTX-induced nephrotoxicity and may be used as a therapeutic adjuvant to ameliorate CTX-induced nephrotoxicity.

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Ameliorative effect of low molecular weight peptides from the head of red shrimp (Solenocera crassicornis) against cyclophosphamide-induced hepatotoxicity in mice

Cited by 27 publications

References 47 publications

Protective Effect of Low Molecular Weight Peptides from Solenocera crassicornis Head against Cyclophosphamide-Induced Nephrotoxicity in Mice via the Keap1/Nrf2 Pathway

Protective Effect of Low Molecular Weight Peptides from Solenocera crassicornis Head against Cyclophosphamide-Induced Nephrotoxicity in Mice via the Keap1/Nrf2 Pathway

Ameliorative Effects of Peptides Derived from Oyster (Crassostrea gigas) on Immunomodulatory Function and Gut Microbiota Structure in Cyclophosphamide-Treated Mice

Ameliorating Effect of Pentadecapeptide Derived from Cyclina sinensis on Cyclophosphamide-Induced Nephrotoxicity

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