Ameliorative Effect of Quercetin against Abamectin-Induced Hemato-Biochemical Alterations and Hepatorenal Oxidative Damage in Nile Tilapia, Oreochromis niloticus

Abstract

Abamectin (ABM) is a common agricultural pesticide and veterinary anthelmintic drug. It can discharge from the sites of application to aquatic systems via surface run-off or spray drift, causing harmful effects to aquatic organisms. The present study investigated the protective effect of dietary quercetin supplementation on hemato-biochemical parameters and hepato-renal oxidative stress biomarkers in Nile tilapia (Oreochromis niloticus) exposed to a sublethal dose of ABM. Fish were allocated into six equal groups. The first group was kept as a control group. The second and third groups (Q400, and Q800) were fed diets supplemented with two quercetin levels (400 and 800 mg/kg diet), respectively. The fourth group (ABM) was intoxicated with 20.73 µg/L of ABM. The fifth and sixth groups (ABM + Q400, and ABM + Q800) were fed diet supplemented with two quercetin levels (400 and 800 mg/kg diet) and simultaneously intoxicated with ABM for 60 days. The results showed that ABM significantly decreased RBCs, hemoglobin content, hematocrit, total protein, albumin levels, and acetylcholinesterase activity activities compared to the control. Meanwhile, ABM significantly increased white blood cells, glucose, total lipids, cholesterol, and alanine and aspartate aminotransferase activities. Liver and kidney levels of lipid peroxidation was significantly increased, while hepato-renal antioxidant biomarkers (reduced glutathione, super oxide dismutase, catalase, and total antioxidant capacity) were significantly decreased upon ABM exposure. On the other hand, quercetin dietary supplementation improved the hemato-biochemical alterations and alleviated oxidative stress induced by ABM exposure. Fish supplemented with quercetin at a level of 800 mg/kg diet showed better alleviating effects against ABM compared to 400 mg/kg diet. Based on these study findings, we suggest that quercetin dietary supplementation (800 mg/kg) offered direct protection against ABM-induced physiological disturbance and oxidative stress in Nile tilapia.

The potential effect of Moringa oleifera ethanolic leaf extract against oxidative stress, immune response disruption induced by abamectin exposure in Oreochromis niloticus

“…The current findings reveal that 28 days of exposure to 0.5 ug/l ABM increased levels of MDA in the brain and liver, as well as SOD, GPx, and GST in liver samples, which explains the causes of disturbance in liver and kidney functions in the ABM group in this study. The results are in line with previous research that showed that ABM can induce oxidative stress and alter the antioxidant response in several fish and shellfish species such as O. niloticus (Mahmoud et al 2021;Mansour et al 2022), Cyprinus carpio (Rohmah et al 2022, Schizothorax prenanti (Hong et al 2020a), and Eriocheir sinensis (Hong et al 2020b). Furthermore, the current research showed that dietary supplementation with MOE mitigated the adverse effects of ABM on the antioxidant system of exposed fish in ABM + MOE group.…”

Ameliorating effects of dietary parsley (Petroselinum crispum) on ammonia toxicity in the rainbow trout, Oncorhynchus mykiss: growth, digestive enzymes, immunity, and stress resistance

“…Plant-based phytobiotics are widely used in aquaculture as natural growth and immune boosting components (Awad and Awaad, 2017;Mohammadi et al, 2020;Al-Shawi et al, 2021;Mansour et al, 2022;Yousefi et al, 2022;Almarri et al, 2023;Mansour et al, 2023). Poten-tials of dietary parsley extract (PUE) were assayed on growth, immunity and resistance of the fish to ammonia stress.…”

A Physiological and Molecular Docking Insight on Quercetin Mediated Salinity Stress Tolerance in Chinese Flowering Cabbage and Increase in Glucosinolate Contents

“…Quercetin is a flavonol phytochemical, with the potential for reducing multiple stress. It is commonly known that quercetin has an ameliorative effect on a variety of environmental stresses in both plants and animals [ 7 , 8 ]. Quercetin facilitates several plant physiological processes, such as seed germination, pollen growth, antioxidant machinery, and photosynthesis, as well as inducing proper plant growth and development [ 9 ].…”