Ameliorative effect of vitamin C against hepatotoxicity induced by emamectin benzoate in rats

Abstract

<jats:p> In the present study, we aimed to assess the potential protective effect of ascorbic acid (AA) against emamectin benzoate (EMB)-induced hepatotoxicity. For this purpose, biochemical, histopathological and analytical investigations were performed. Male Wistar rats were distributed into three groups, that is, a control group, an EMB group given 10 mg EMB/kg body weight (BW) by gavage and an EMB + AA group given 10 mg EMB/kg BW and vitamin C intraperitoneally (200 mg/kg). The duration of the treatment was 28 days and the duration of the study was 42 days. There was a statistically significant increase of all hepatic biomarkers, that is, aspartate aminotransferase, alanine aminotransferase and gamma-glutamyltransferase activities, and glycemia, in EMB-treated group when compared with the control group. Light microscopic observations revealed variable signs of hepatotoxicity in the EMB group, which were represented by alteration of normal hepatic architecture, inflammatory cell infiltration, hepatocellular steatosis and foci of necrosis at 28 and 42 days post-treatment. However, co-treatment with vitamin C reduced EMB-related liver toxicity and diminished the abnormal biochemical and architectural damage. Emamectin B1a and B1b residues were detectable in all plasma samples of treated rats at 14, 21 and 28 days of treatment. The drug liver tissue concentration was significantly lower in EMB + AA group compared with EMB group at 28 and 42 days. In conclusion, the findings of the present study clearly indicate a significant protective action of vitamin C against EMB hepatotoxicity. </jats:p>

Antioxidant Activity of Some Natural Compounds in Alleviating the Hepatotoxicity Effects Induced by Emamectin Benzoate in Male Mice

“…The ability of cinnamon inhibited DNA damage may be due to its containing of high flavonoid and polyphenol compounds (Kumar et al, 2012). The results belong to histopathological studies with EMB treatment agreed with El-Sheikh and Galal (2015), Khaldoun et al (2017) and Abou-Zeid et al (2018), who found that emamectin administration produced pathological changes in the liver. These changes include blood vessel congestion with infiltration of lymphocytes, necrosis with hepatic parenchyma infiltration, also, dilated sinusoids.…”

“…This increase is sustaining the hypothesis that exposure to pesticide resulted in toxicity against biochemical liver (Hernández et al, 2013). The results diagrammatic in Figure ( 4) agreed with Khaldoun et al (2017), who reported a markedly elevation of AST and ALT levels in the plasma of EMB-treated rats. These alterations of AST and ALT activity may be due to necrotic-changes of hepatic tissue that appear in histopathological examination (Figure .…”

“…5 ). Khaldoun et al (2017) suggested that EMB toxicity is possibly caused by oxidative stress. The BS treatment combined with EMB leads to recovery and return to the normal appearance of hepatocytes and central vein.…”

Boldenone Undecylenate-Mediated Hepatorenal Impairment by Oxidative Damage and Dysregulation of Heat Shock Protein 90 and Androgen Receptors Expressions: Vitamin C Preventive Role

“…Moreover, Bashandy and AlWasel (2011) described that VC normalized ALT and AST levels in rats' liver intoxicated with carbon tetrachloride. Besides, VC reduced necrosis and restored the normal appearance and structure of damaged hepatocytes due to emamectin benzoate exposure (Khaldoun Oularbi et al, 2017).…”

The Use of Chitosan-Coated Nanovesicles in Repairing Alcohol-Induced Damage of Liver Cells in Mice

“…Ascorbic acid (200 mg/kg body weight) is used as a positive control in the experiment. Its protective effects on the liver are wellknown in mice with toxic liver disease induced by drugs or by various chemicals [41][42][43][44][45][46][47][48].…”