Ameliorative Effects of Cardamonin on Monosodium Urate-Induced Gouty Arthritis through Inhibiting NLRP3 Inflammasome Mediation

Wang, Chih Chien; Lu, Jeng‐Wei; Peng, Yi‐Jen; Lee, Chian-Her; Lee, Herng‐Sheng; Chu, You-Hsiang; Huang, Chun-Jung; Ho, Yi‐Jung; Liu, Feng‐Cheng; Wu, Chia-Chun

doi:10.3390/medicina57090898

Cited by 9 publications

(5 citation statements)

References 40 publications

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“…Potassium oxazinate + hypoxanthine powder was mixed with a .5% sodium carboxymethyl cellulose (CMC Na) suspension. 20 At the 3rd and 4th weeks, the serum uric acid levels were measured. 21 When the rat serum uric acid level reached 110 μmol/L, the model was successfully established.…”

Section: In Vivo Experimental Protocolmentioning

confidence: 99%

Tongbixiao Pills Improve Gout by Reducing Uric Acid Levels and Inhibiting Inflammation

ZhiguoShao

et al. 2022

Dose-Response

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Gout is a chronic disease. Gout symptoms are often experienced in the middle of the night. The onset of gout in the middle of the night is closely related to abnormal liver and gallbladder meridian. The purpose of this study was to investigate the clinical efficacy and possible mechanism of action of Tongbixiao pills in the treatment of hyperuricemia and gouty arthritis. The Tongbixiao pills we used included several traditional Chinese medicines, most of which tonify the spleen; strengthen the liver; benefit the kidney; and reduce heat, dampness, and blood stasis. In this randomized clinical study of 105 patients, we found that Tongbixiao pills can reduce uric acid levels in hyperuricemia patients. Additionally, the efficacy was similar to that of allopurinol and the level of uric acid did not increase significantly at eight weeks after withdrawal. In the absence of notable adverse reactions, Tongbixiao pills can also increase uric acid excretion, reduce serum creatinine and lipid levels, and reduce inflammation and relieve gout symptoms. In addition, we used SD rats to simulate gout and arthritis and divided them into five groups: normal group, model group, low-dose group, medium-dose group, and high-dose group. The inflammatory indices of the 40 SD rats were observed. After seven days, ankle swelling in rats in the treatment group was significantly reduced. The indices of uric acid, creatinine, and urea nitrogen in the treatment group were significantly lower than those in the model group, which proved that Tongbixiao pills could inhibit hyperuricemia in rats, thus treating gout. This study demonstrates that Tongbixiao pills can treat gout, provide more treatment options for gouty arthritis, and improve the quality of life of patients.

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Section: In Vivo Experimental Protocolmentioning

confidence: 99%

Tongbixiao Pills Improve Gout by Reducing Uric Acid Levels and Inhibiting Inflammation

ZhiguoShao

et al. 2022

Dose-Response

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“…T A B L E 3 Identification of potential biomarkers in kidney tissue. PTGS, also known as COX, is the key enzyme in the initial step of PG synthesis in organisms (Chen et al, 2019) and the precursor of several bioactive lipid regulator families, including PG, thromboxane and prostacyclin (Wang et al, 2021). COX-1/2 is one of the key enzymes involved in AA metabolism (Das, 2022), and COX-1 is responsible for the expression of prostaglandin E2 (PGE2) in response to stimulation of hypotonic swelling.…”

Section: Discussionmentioning

confidence: 99%

Integrating network pharmacology and renal metabonomics to reveal the protective mechanism of resveratrol on gouty nephropathy

Xu,

Yu,

Wang

et al. 2024

Biomedical Chromatography

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Resveratrol (Res) has been demonstrated to have beneficial effects on gouty nephropathy (GN). However, the mechanisms of Res on GN remain unclear. This study aimed to investigate the mechanisms of Res on GN. In this study, network pharmacology technology was used to predict the Res targets in the prevention and treatment of GN. Renal metabonomics was used to identify differential metabolites in kidney tissue of GN model rats. Finally, molecular docking technology was used to verify the binding ability of Res to key targets. Metabonomics analysis showed that 24 potentially important metabolites were involved in the prevention and treatment of GN with Res. After exposure to Res, metabolite levels normalized. The network pharmacology analysis showed that 24 key targets were involved in the prevention and treatment of GN disease. According to the metabolite–gene network diagram, we identified two core genes, PTGS1 and PTGS2, and found that both were involved in the arachidonic acid metabolism pathway. Molecular docking further verified the affinity of Res binding to PTGS1 and PTGS2. In conclusion, the mechanism of Res against GN may be the regulation of arachidonic acid metabolism through the regulation of PTGS 1 and PTGS 2.

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“…* P < 0.05 and ** P < 0.01; # P < 0.05 and ## P < 0.01 was chosen to be statistically significant, respectively. 21 …”

Section: Methodsmentioning

confidence: 99%

Anti-Inflammatory and Chondro-Protective Effects of Acidic Polysaccharide from Enteromorpha Prolifera in Experimental Models of Osteoarthritis In-Vitro and In-Vivo

Wang

Chiang

et al. 2022

CARTILAGE

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Objective Osteoarthritis (OA) progression has been shown to increase the expression of inflammatory cytokines in joints, leading to the destruction of cartilage matrix. Interleukin (IL)-1β is a potent inflammatory cytokine associated with osteoarthritic synovial fluid. The protective effects of polysaccharides from Enteromorpha prolifera against acute hepatic injury was reported. Design In this study, we examined the effects of Enteromorpha polysaccharide extracts (EPEs) in the treatment of OA. The effects of the EPEs were assessed using an IL-1β-stimulated SW1353 and SW982 cells. The expression levels of specific mRNA and proteins were evaluated using semi-quantitative reverse transcription polymerase chain reaction (sqRT-PCR) and western immunoblotting. An OA animal study involving C57BL/6J mice was also conducted to assess the effects on tactile sensitivity and anterior cruciate ligament transection (ACLT). Results Acidic polysaccharide extract (APE) was shown to significantly reduce cytokine and chemokine mRNA levels in IL-1β-stimulated SW1353 and SW982 cells and attenuate the expression of proinflammatory cytokines and p38/AP-1 in SW1353 cells. APE was also shown to minimize the effect of osteolytic lesions in the knee joints of ACLT-induced osteoarthritic mice. Conclusions APE is a potent inhibitor of joint degeneration associated with OA.

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Ameliorative Effects of Cardamonin on Monosodium Urate-Induced Gouty Arthritis through Inhibiting NLRP3 Inflammasome Mediation

Cited by 9 publications

References 40 publications

Tongbixiao Pills Improve Gout by Reducing Uric Acid Levels and Inhibiting Inflammation

Tongbixiao Pills Improve Gout by Reducing Uric Acid Levels and Inhibiting Inflammation

Integrating network pharmacology and renal metabonomics to reveal the protective mechanism of resveratrol on gouty nephropathy

Anti-Inflammatory and Chondro-Protective Effects of Acidic Polysaccharide from Enteromorpha Prolifera in Experimental Models of Osteoarthritis In-Vitro and In-Vivo

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