Ameliorative effects of Saussurea lappa root aqueous extract against Ethephon‐induced reproductive toxicity in male rats

Abstract

This study was designed to evaluate protective effect of Saussurea lappa root aqueous extract against Ethephon (2-chloroethylphosphonic acid)-induced reproductive toxicity in rats. Control group received distilled water. Second group was given S. lappa extract at a dose 50 mg/kg bw.Third group was given Ethephon at a dose 200 mg/kg bw. Fourth, fifth, and sixth groups were given S. lappa extract before, with or after Ethephon administration, respectively. Ethephon intoxication significantly decreased serum levels of follicle stimulating hormone, luteinizing hormone, testosterone, and prolactin. Also, it significantly decreased sperms count, vitality, morphology index, total motility, progressive motility, and proliferating cell nuclear antigen protein expressions in spermatogonia. However, it significantly increased sperms abnormalities, testicular tissue and DNA damages, P53 protein expressions, noprogressive motility, and immotile sperms. In contrast, S. lappa extract ameliorated these alterations. These results indicated that S. lappa had potential preventive and curative effects against Ethephon-induced reproductive toxicity in rats.

Ameliorative effects of vitamin B17 on the kidney against Ehrlich ascites carcinoma induced renal toxicity in mice

“…The damage of the kidney DNA using analysis and quantification conducted with the comet assay was examine according to the method of Abd Eldaim et al…”

“…Protein expression of apoptotic p53 in the liver section was detected by using ABC (Elite‐ABC, Vector Laboratories) method against P53 antibody (dilution 1:200 DAKO Japan Co, Ltd., Tokyo, Japan) according to Tousson et al and Abd Eldaim et al…”

“…Despite the significant development of anticancer treatments, the incidence of cancer is still increasing worldwide. However, cancer prevention by using naturally occurring dietary substances has been considered as a practical approach to reducing the ever‐growing incidence of cancer …”

“…However, cancer prevention by using naturally occurring dietary substances has been considered as a practical approach to reducing the ever-growing incidence of cancer. [11][12][13][14][15][16] According to numerous studies, plant ingredients and their extracts were used in anticancer, anti-inflammation, reducing renal and hepatotoxicity, and treat many diseases. [17][18][19][20][21] Vitamin B17 (VB17), amygdalin and formerly called laetrile is one of many nitrilosides which are natural cyanide containing substances, which is abundant in the seeds of Prunasins family, such as apricots, apples, almonds, peaches, and other rosaceous plants.…”

Modulatory effects of Saussurea lappa root aqueous extract against ethephon‐induced kidney toxicity in male rats

“…Plant growth promoter ethephon has been reported to induce damage to the kidneys and liver . It has been reported to induce reproductive toxicity in rats . Ethephon has been reported to cause toxic, mutagenic, and teratogenic effects; found to reduce DNA and RNA concentrations; and also found to inhibit cholinesterase enzyme activity .…”