Ameliorative Effects of Trypadim®, Trypamidium® and Novidium® on the Negative Effects on Body Temperature and Serum Chemistry in Wistar Rats Infected With Trypanosoma Brucei Brucei (Federe Strain)

Abstract

Effect of intramuscular administration of Trypanocides on body temperature and serum chemistry in Wistar rats infected with Trypanosoma brucei brucei was investigated. 25 Wistar rats with average body weights of 200-240±20 g were randomly divided into five groups. Group A was administered 0.5 mL of normal saline only, while group B was given 0.1 × 106 of T. brucei brucei only. Group C, D and E were inoculated with the same dose of the parasite as in group B and in addition, they were intramuscularly administered with 3.5 mg/kg b.w. of Trypadim® (Diminazene di-aceturate),1 mg/kg b.w. of Trypamidium® (Isometamidium chloride) and 1 mg/kg b.w. of Novidium® (Homidium chloride), respectively. Body temperature increased consistently in all groups except group A. However, there was a significant (p<0.05) difference in all treated groups compared to group B on 28day post-infection (DPI). All serum indicators increased significantly (p<0.05) in group B when compared to all other groups. Values observed for creatinine in all treated groups was only significantly (p<0.05) higher in group E when compared to group A. In conclusion, administered trypanocidal drugs possess anti-pyrexia activity and could off-set the negative effect of T.brucei.brucei on the measured serum chemistry in Wistar rats.

Phytochemical Screening, GC-MS Analysis, and Evaluating In Vivo Antitrypanosomal Effects of a Methanolic Extract of Garcinia kola Nuts on Rats

“…The body temperature of all experimental animals was measured daily. Each rat was gently trapped, and a digital thermometer (Biobase, Jinan, China) was inserted 3 cm into the anus of each rat upon hearing a beep, the thermometer was immediately withdrawn, and the values obtained were recorded as described by Ajakaiye et al [ 39 ].…”