Ameliorative Efficacy Of Tribulus Terrestris Fruit Extract On Female Reproductive Hormones: An Interactive Study Of Co-Induced Diabetes And Cold Stress

Abstract

Diabetes existed as a global disease and people residing in cold temperature areas encounter synergetic effects resulting in exacerbation of diabetic impediments. In view of dearth in literature with respect to its synergistic actions, this study was intended to discover an interactive effect of co-induced diabetes and cold stress on female rat reproductive hormones and also to assess ameliorative role of both Tribulus terrestris fruit (TTF) extract. STZ induced (45mg/kg bw) diabetic rats were exposed to cold stress 4±2ºC to explore the alterations in female reproductive hormones. A group of experimental rats were supplemented with TTF and extract individually at doses of 150, 200, 250 mg/kg bw for 19 days respectively. Significant alterations occurred in the levels of gonadal hormones in experimental rats’ advocates exacerbation in toxicity due to cold stress. Both diabetes and cold stress alone found to decrease FSH, LH and estrogen levels significantly (P<0.05), while progesterone levels increased significantly. Contrary to supra, cold exposure to diabetic rats caused augmentations in FSH, LH and progesterone levels with decreased estrogen levels. Supplementation of both TTF extract was found to be advantageous in reinstating the altered gonadal hormones as witnessed in cold stressed diabetic rats. The ameliorative efficacy of phytoextract could be due to the additive/individual ability of polyphenols, flavonoids and steroidal saponins which are known for hormone regulatory actions and also antidepressant actions suggesting their therapeutic role in both cold stressed and diabetic population.