Ameliorative potential of vitamin C and E against Roundup‐glyphosate induced genotoxicity triggering apoptosis in caprine granulosa cells

Bhardwaj, Jitender Kumar; Mittal, Mandeep; Saraf, Priyanka; Sharma, Sanjeev Kumar

doi:10.1002/em.22497

Cited by 10 publications

(5 citation statements)

References 49 publications

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“…Table 1 reports the baseline characteristics of the participants, in the present study, there were 342 samples; primary type of infertility was higher than the secondary cause. With median age of 22 (19)(20)(21)(22)(23)(24)(25)(26) years at the time of marriage, the duration of marriage was 7(4-11) years. Results showed that the highest cause of infertility was due to PCOS followed by endometriosis.…”

Section: Resultsmentioning

confidence: 99%

“…that protects the cell from OS and also preserves other antioxidants such as vitamins C and E; the role of these vitamins has been already documented [19]. Visfatin is an adipokine released from visceral adipose tissue dominantly [20].…”

Section: Plos Onementioning

confidence: 99%

“…Glutathione is a family of enzymes that includes glutathione peroxidase (GPx), glutathione S-transferase (GST), and glutathione reductase (GSH) [ 18 ]. It is an endogenous antioxidant that protects the cell from OS and also preserves other antioxidants such as vitamins C and E; the role of these vitamins has been already documented [ 19 ]. Visfatin is an adipokine released from visceral adipose tissue dominantly [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

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SIRT1 and antioxidants in infertile females: Exploration of the role of vitamin D

et al. 2023

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Background Deficiency of silent information regulator 1 (SIRT1) can trigger inflammation, mitochondrial malfunctioning, and apoptosis through the hypothalamic-pituitary-ovarian axis, producing poor quality oocytes, leading to infertility. Normal vitamin D (VD) levels promote SIRT1 activity required for optimal fertility, and low levels of either may result in fertility problems owing to cell-membrane de-stabilization, increased autophagy, DNA damage leading to increased reactive oxygen species and mitochondrial dysfunction. Therefore, in this study, we want to estimate the levels of VD, SIRT1 and antioxidants (MnSOD; manganese superoxide dismutase, GR; glutathione reductase, visfatin) and oxidants (adrenaline & cortisol) in individuals living with infertility and explore the association of VD with SIRT1 expression (levels), antioxidants, and oxidants contributing to infertility in women. The significance of this study is that it highlights the importance of maintaining optimal levels of VD for reproductive health in females. Methods This cross-sectional study included 342 (135 infertile and 207 fertile) female subjects. Serum levels of MnSOD, SIRT1, visfatin, GR, VD, adrenaline, and cortisol were analyzed by ELISA and were compared in fertile and infertile samples using the Mann Whitney U test. Results There were significantly high levels of VD, SIRT1, GR, MnSOD and visfatin in fertile female participants. However, mean adrenaline and cortisol levels were higher in infertile samples with a significant negative correlation with VD. A significant negative correlation of VD with MnSOD, SIRT1, visfatin and GR was observed (p <0.01). In VD subset groups, MnSOD levels were significantly high in VD sufficient groups however, adrenaline and cortisol levels were significantly high in groups suffering from VD deficiency. Conclusions Deficiency of VD is associated with a decrease in SIRT1 and other antioxidants, which may deter natural reproductive functions leading to infertility. Further studies are required to determine the cause-effect relationship of VD deficiency on conception and interpretation of the involved mechanism.

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Section: Resultsmentioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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SIRT1 and antioxidants in infertile females: Exploration of the role of vitamin D

et al. 2023

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“…It is impossible to know if the newly recruited oocytes would have been ovulated because, in Podarcis, the maximum number of eggs is strictly controlled and depends on female size more than on environmental cues [ 68 , 70 ]. It is, however, quite interesting that no atresia was observed, though treatment lasted for 3 weeks, and glyphosate induces follicular granulosa degeneration [ 6 ]. In Podarcis, follicle selection occurs in early diplotene, before the formation of the primordial follicle [ 71 ], a convenient strategy for a small species that produces large and telolecithal eggs.…”

Section: Discussionmentioning

confidence: 99%

“…Gly has been found in human urine following its ingestion through contaminated vegetables [ 2 , 3 ]. Experimental exposure of mammals to Gly had mutagenic and carcinogenic effects [ 4 , 5 , 6 ], causing neurotoxicity, hepatotoxicity, and disruption of the reproductive axis [ 1 , 7 ]. As a consequence, fertility is greatly reduced by Gly; implantation failures [ 8 ] and teratogenesis [ 9 ] have been detected, as well as interferences with both spermatogenesis and oogenesis [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Glyphosate Interference in Follicular Organization in the Wall Lizard Podarcis siculus

Rosati

Chianese

Gregorio

et al. 2023

IJMS

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Glyphosate (Gly) is a broad-spectrum herbicide widely used thanks to its high efficiency and low toxicity. However, evidence exists of its toxic effects on non-target organisms. Among these, the animals inhabiting agricultural fields are particularly threatened. Recent studies demonstrated that exposure to Gly markedly affected the morphophysiology of the liver and testis of the Italian field lizard Podarcis siculus. The present study aimed to investigate the effects of the herbicide on the female reproductive system of this lizard in order to have a full picture of Gly-induced reproductive impairment. The animals were exposed to 0.05 and 0.5 μg/kg of pure Gly by gavage for 3 weeks. The results demonstrated that Gly, at both doses tested, profoundly interfered with ovarian function. It induced germ cells’ recruitment and altered follicular anatomy by anticipating apoptotic regression of the pyriform cells. It also induced thecal fibrosis and affected oocyte cytoplasm and zona pellucida organizations. At the functional levels, Gly stimulated the synthesis of estrogen receptors, suggesting a serious endocrine-disrupting effect. Overall, the follicular alterations, combined with those found at the level of the seminiferous tubules in males, suggest serious damage to the reproductive fitness of these non-target organisms, which over time could lead to a decline in survival.

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Attenuation of sodium arsenite mediated ovarian DNA damage, follicular atresia, and oxidative injury by combined application of vitamin E and C in post pubertal Wistar rats

Mondal

Pal

Biswas

et al. 2023

Naunyn-Schmiedeberg's Arch Pharmacol

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Ameliorative potential of vitamin C and E against Roundup‐glyphosate induced genotoxicity triggering apoptosis in caprine granulosa cells

Cited by 10 publications

References 49 publications

SIRT1 and antioxidants in infertile females: Exploration of the role of vitamin D

SIRT1 and antioxidants in infertile females: Exploration of the role of vitamin D

Glyphosate Interference in Follicular Organization in the Wall Lizard Podarcis siculus

Attenuation of sodium arsenite mediated ovarian DNA damage, follicular atresia, and oxidative injury by combined application of vitamin E and C in post pubertal Wistar rats

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